Kojic acid production in an airlift bioreactor using partially hydrolyzed raw corn starch

Futamura, Takafumi; ISHIHARA, HIRONORI; Tamura, Takayoshi; Yasutake, Tetsuya; Huang, Guowei; KOJIMA, MAMI; Okabe, Mitsuyasu

doi:10.1016/s1389-1723(01)80240-1

Cited by 14 publications

(9 citation statements)

References 9 publications

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“…It is known that KA production is dependent on the type and concentration of the nitrogen source (Wan et al, 2005;Futamura et al, 2001a). We found that the addition of a small amount of sodium nitrate in the standard medium (final concentration of 0.1%) resulted in complete loss of KA production (Fig.…”

Section: Ka Production By a Oryzaementioning

confidence: 70%

“…Addition of 0.1-0.25% yeast extract to the medium increased cell growth and KA production, but addition of >0.8% yeast extract resulted in markedly reduced biomass formation and lower KA production (Wan et al, 2005;Futamura et al, 2001a). Therefore, medium containing 0.25% yeast extract, which was optimum for KA production according to these previous reports, was used unless otherwise indicated.…”

Section: Strains and Mediamentioning

confidence: 99%

See 1 more Smart Citation

Identification and characterization of genes responsible for biosynthesis of kojic acid, an industrially important compound from Aspergillus oryzae

Terabayashi

Sano

Yamane

et al. 2010

Fungal Genetics and Biology

155

153

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Section: Ka Production By a Oryzaementioning

confidence: 70%

Section: Strains and Mediamentioning

confidence: 99%

Identification and characterization of genes responsible for biosynthesis of kojic acid, an industrially important compound from Aspergillus oryzae

Terabayashi

Sano

Yamane

et al. 2010

Fungal Genetics and Biology

155

153

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“…The pH value decreased during cell growth until reaching stationary growth phase, after remaining constant between 72 to 132 h. The drop in pH may be attributed to the production of organic acids, which were consumed during later stages of growth resulting in a slight pH increase to 4.0 (Futamura et al 2001). These results show that alkalinization of the medium is closely related with the degradation of extracellular proteins by proteases produced from Streptomyces sp DPUA1576, suggesting that alkalinization is adaptive.…”

Section: Discussionmentioning

confidence: 75%

Screening, production and biochemical characterization of a new fibrinolytic enzyme produced by Streptomyces sp. (Streptomycetaceae) isolated from Amazonian lichens

et al. 2016

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Thrombosis is a pathophysiological disorder caused by accumulation of fibrin in the blood. Fibrinolytic proteases with potent thrombolytic activity have been produced by diverse microbial sources. Considering the microbial biodiversity of the Amazon region, this study aimed at the screening, production and biochemical characterization of a fibrinolytic enzyme produced by Streptomyces sp. isolated from Amazonian lichens. The strain Streptomyces DPUA1576 showed the highest fibrinolytic activity, which was 283 mm 2 . Three variables at two levels were used to assess their effects on the fibrinolytic production. The parameters studied were agitation (0.28 -1.12 g), temperature (28 -36 ºC) and pH (6.0 -8.0); all of them had significant effects on the fibrinolytic production. The maximum fibrinolytic activity (304 mm 2 ) was observed at 1.12 g, 28 ºC, and pH of 8.0. The crude extract of the fermentation broth was used to assess the biochemical properties of the enzyme. Protease and fibrinolytic activities were stable during 6 h, at a pH ranging from 6.8 to 8.4 and 5.8 to 9.2, respectively. Optimum temperature for protease activity ranged between 35 and 55 °C, while the highest fibrinolytic activity was observed at 45 ºC. Proteolytic activity was inhibited by Cu 2+ and Co 2+ ions, phenylmethylsulfonyl fluoride (PMSF) and pepstatin A, which suggests that the enzyme is a serine protease. Enzymatic extract cleaved fibrinogen at the subunits Aα-chain, Aβ-chain, and γ-chain. The results indicated that Streptomyces sp. DPUA 1576 produces enzymes with fibrinolytic and fibrinogenolytic activity, enzymes with an important application in the pharmaceutical industry. KEYWORDS: Actinomycetes, fibrinolytic protease, inhibitor protease, fibrinogenolytic activity.Seleção, produção e caracterização bioquímica de uma nova enzima fibrinolítica produzida por Streptomyces sp. (Streptomycetaceae) isolada de liquens da Amazônia RESUMO A trombose é uma doença patofisiológica causada pelo acúmulo de fibrina no sangue. Proteases fibrinolíticas com potente atividade trombolítica são produzidas por diversas fontes microbianas. Considerando a biodiversidade microbiana da região amazônica, o presente estudo teve como objetivo a seleção, produção e caracterização bioquímica da enzima fibrinolítica de Streptomyces sp. isolado de líquens da Amazônia. Streptomyces DPUA1576 foi a melhor produtora com atividade fibrinolítica de 283 mm 2 . Três variáveis em dois níveis foram utilizadas para determinar as variáveis mais relevantes na produção da enzima fibrinolítica (FA). Os parâmetros estudados foram agitação (0.28 -1.12 g), temperatura (28 -36 ºC) e pH (6.0 -8.0) e todos obtiveram efeitos significativos na produção fibrinolítica. A maior atividade fibrinolítica (304 mm 2 ) foi obtida a 1.12 g, 28 ºC e pH 8.0. O extrato bruto da fermentação foi usado para determinar as propriedades bioquímicas da enzima. Atividades proteásica e fibrinolítica foram estáveis durante 6 horas no intervalo de pH entre 6.8 -8.4 e 5.8 -9.2, respectivamente. Temperatura ...

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“…In the present study the production cost of kojic acid was approximately USD 6.25 with 1 kg of Sago starch substrate and 285 g of kojic acid crystals were obtained. According to Futamura et al [11] the production cost of kojic acid was USD 9.50 with substrate Corn starch. So the production cost of kojic acid when compared to Futamura et al [11] was reduced by 35% in the present study.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Cultural Parameters for Cost Effective Production of Kojic Acid by Fungal Species Isolated from Soil

Kb¹,

Vijayalakshmi²,

Shilpa³

et al. 2015

Ferment Technol

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A novel isolate capable of producing opulent yields of kojic acid with surface fermentation was screened from ten different isolated soil fungi. The organism was confirmed as Aspergillus flavus FJ537130 strain using 18SrRNA based molecular analysis and it was identified as a negative producer of aflatoxin. Though twelve different carbon sources were used, significant yields of kojic acid crystals was noticed with Sago starch. The optimized conditions established were substrate concentration 1000 ml (100 g of starch powder in 1000 ml of H 2 O), pH 6.0, Time 28 d, Temperature 28°C, Peptone concentration 4 g/l, KH 2 PO 4 concentration 1 g/l, MgSO 4 concentration 0.5 g/l and the yield achieved was 28.5 g/l. The resulted fermentation broth was subjected to solvent extraction followed by gel filtration for the separation of kojic acid and crystallization. The structural characterization of purified kojic acid was confirmed by Proton NMR, FTIR and XRD. The molecular weight and purity of kojic acid was confirmed by LC/MS and HPLC. The isolated kojic acid crystals shows high antimicrobial activity against pathogenic bacteria Staphylococcus aureus and Escherichia coli and maximum zone of inhibition was 9 mm. The inhibitory effect of kojic acid was more on the cell line K562 (Leukemia) when compared to the MDAMB435S (Breast cancer) cell line.

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Kojic acid production in an airlift bioreactor using partially hydrolyzed raw corn starch

Cited by 14 publications

References 9 publications

Identification and characterization of genes responsible for biosynthesis of kojic acid, an industrially important compound from Aspergillus oryzae

Identification and characterization of genes responsible for biosynthesis of kojic acid, an industrially important compound from Aspergillus oryzae

Screening, production and biochemical characterization of a new fibrinolytic enzyme produced by Streptomyces sp. (Streptomycetaceae) isolated from Amazonian lichens

Optimization of Cultural Parameters for Cost Effective Production of Kojic Acid by Fungal Species Isolated from Soil

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