Kojic Acid Production in an Airlift Bioreactor Using Partially Hydrolyzed Raw Corn Starch.

Futamura, Takafumi; ISHIHARA, HIRONORI; Tamura, Takayoshi; Yasutake, Tetsuya; Huang, Guowei; KOJIMA, MAMI; Okabe, Mitsuyasu

doi:10.1263/jbb.92.360

Cited by 8 publications

(4 citation statements)

References 9 publications

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“…They attribute this reduction to degradation of kojic acid to oxalic and acetic acid by the mycelium under glucose depleted conditions [23, 31, 54, 55]. Futamura et al [56] produced kojic acid by A. oryzae MK-107-39 in a jar fermentor and recorded that level of kojic acid reached up to 40 g/L. On the other hand, Wakisaka et al [42] produced kojic acid by A. oryzae NRRL 484 using continuous membrane surface liquid culture technique and recorded that kojic acid concentration reached 45 mg/mL medium and nearly constant after 15 days of cultivation for over 70 days.…”

Section: Resultsmentioning

confidence: 99%

Kojic Acid Production from Agro-Industrial By-Products Using Fungi

El-Kady

Zohri

Hamed

2014

Biotechnology Research International

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A total of 278 different isolates of filamentous fungi were screened using synthetic medium for respective ability to produce kojic acid. Nineteen, six, and five isolates proved to be low, moderate, and high kojic acid producers, respectively. Levels of kojic acid produced were generally increased when shaking cultivation was used rather than those obtained using static cultivation. A trial for the utilization of 15 agro-industrial wastes or by-products for kojic acid production by the five selected higher kojic acid producer isolates was made. The best by-product medium recorded was molasses for kojic acid. A. flavus numbers 7 and 24 were able to grow and produce kojic acid on only 12 out of 15 wastes or by-products media. The best medium used for kojic acid production by A. flavus number 7 was rice fragments followed by molasses, while the best medium used for kojic acid production by A. flavus number 24 was the molasses followed by orange, pea, and rice fragments. An attempt for production of kojic acid using a 1.5 L laboratory fermentor has been made. Aspergillus flavus number 7 was used and grown on molasses medium; maximum level (53.5 g/L) of kojic acid was obtained after eight days of incubation.

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Section: Resultsmentioning

confidence: 99%

Kojic Acid Production from Agro-Industrial By-Products Using Fungi

El-Kady

Zohri

Hamed

2014

Biotechnology Research International

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“…In the present study, the transcription level of the enzyme and the transporter were enhanced by an over-expression of the transcription factor, which correlates well with that report. Although there are reports of improved kojic acid productivity through fermentation engineering strategies [1,5,23], to our knowledge, this study is the first to report an improvement in kojic acid productivity via genetic engineering. Kojic acid productivity could be improved even more by combining fermentation engineering and genetic engineering in future work.…”

Section: Discussionmentioning

confidence: 84%

“…Thus, it has some unique properties. Futamura et al [5] reported that the production cost of kojic acid is about $10/kg. It must be decreased to less than $2/kg before it could be used in many applications.…”

Section: Introductionmentioning

confidence: 99%

Aspergillus oryzae-based cell factory for direct kojic acid production from cellulose

et al. 2014

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BackgroundKojic acid (5-Hydroxy-2-(hydroxymethyl)-4-pyrone) is one of the major secondary metabolites in Aspergillus oryzae. It is widely used in food, pharmaceuticals, and cosmetics. The production cost, however, is too high for its use in many applications. Thus, an efficient and cost-effective kojic acid production process would be valuable. However, little is known about the complete set of genes for kojic acid production. Currently, kojic acid is produced from glucose. The efficient production of kojic acid using cellulose as an inexpensive substrate would help establish cost-effective kojic acid production.ResultsA kojic acid transcription factor gene over-expressing the A. oryzae strain was constructed. Three genes related to kojic acid production in this strain were transcribed in higher amounts than those found in the wild-type strain. This strain produced 26.4 g/L kojic acid from 80 g/L glucose. Furthermore, this strain was transformed with plasmid harboring 3 cellulase genes. The resultant A. oryzae strain successfully produced 0.18 g/L of kojic acid in 6 days of fermentation from the phosphoric acid swollen cellulose.ConclusionsKojic acid was produced directly from cellulose material using genetically engineered A. oryzae. Because A. oryzae has efficient protein secretion ability and secondary metabolite productivity, an A. oryzae-based cell factory could be a platform for the production of various kinds of bio-based chemicals.

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“…Instead, the three genes encode an oxidoreductase, a Zn(II) 2 -Cys 6 (C6)-type transcription factor, and a major facilitator superfamily transporter [10], [11]. KA production is typically observed after 3 to 4 days of inoculation of A. oryzae in liquid growth media, and can be stopped by adding a small amount of sodium nitrate to the medium [21], [22].…”

Section: Resultsmentioning

confidence: 99%

MIDDAS-M: Motif-Independent De Novo Detection of Secondary Metabolite Gene Clusters through the Integration of Genome Sequencing and Transcriptome Data

et al. 2013

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Many bioactive natural products are produced as “secondary metabolites” by plants, bacteria, and fungi. During the middle of the 20th century, several secondary metabolites from fungi revolutionized the pharmaceutical industry, for example, penicillin, lovastatin, and cyclosporine. They are generally biosynthesized by enzymes encoded by clusters of coordinately regulated genes, and several motif-based methods have been developed to detect secondary metabolite biosynthetic (SMB) gene clusters using the sequence information of typical SMB core genes such as polyketide synthases (PKS) and non-ribosomal peptide synthetases (NRPS). However, no detection method exists for SMB gene clusters that are functional and do not include core SMB genes at present. To advance the exploration of SMB gene clusters, especially those without known core genes, we developed MIDDAS-M, a motif-independent de novo detection algorithm for SMB gene clusters. We integrated virtual gene cluster generation in an annotated genome sequence with highly sensitive scoring of the cooperative transcriptional regulation of cluster member genes. MIDDAS-M accurately predicted 38 SMB gene clusters that have been experimentally confirmed and/or predicted by other motif-based methods in 3 fungal strains. MIDDAS-M further identified a new SMB gene cluster for ustiloxin B, which was experimentally validated. Sequence analysis of the cluster genes indicated a novel mechanism for peptide biosynthesis independent of NRPS. Because it is fully computational and independent of empirical knowledge about SMB core genes, MIDDAS-M allows a large-scale, comprehensive analysis of SMB gene clusters, including those with novel biosynthetic mechanisms that do not contain any functionally characterized genes.

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Kojic Acid Production in an Airlift Bioreactor Using Partially Hydrolyzed Raw Corn Starch.

Cited by 8 publications

References 9 publications

Kojic Acid Production from Agro-Industrial By-Products Using Fungi

Kojic Acid Production from Agro-Industrial By-Products Using Fungi

Aspergillus oryzae-based cell factory for direct kojic acid production from cellulose

MIDDAS-M: Motif-Independent De Novo Detection of Secondary Metabolite Gene Clusters through the Integration of Genome Sequencing and Transcriptome Data

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