Kolagen dari Limbah Tulang Ayam (Gallus gallus domesticus) terhadap Aktivitas Anti Aging secara In Vitro

Abstract

<p>Limbah tulang ayam meningkat seiring dengan peningkatan konsumsi ayam. Namun, belum banyak penelitian yang memanfaatkan limbah tulang sebagai sumber kolagen. Penelitian ini bertujuan mengetahui pengaruh ukuran kolagen terhadap aktivitas anti aging berupa aktivitas antioksidan, antiglikasi, dan inhibitor tirosinase secara <em>in vitro</em> dan mendapatkan teknik isolasi kolagen <em>anti aging</em> optimum dari tulang ayam. Isolasi kolagen dilakukan dengan variasi konsentrasi NaOH, yaitu 0,05 M; 0,10 M; dan 0,20 M, dilanjutkan dengan perendaman menggunakan asam asetat 1 M. Kolagen yang diisolasi dengan NaOH 0,10 M merupakan kolagen dengan ukuran partikel, rendemen, dan antiglikasi terbesar (berturut-turut 2,34 µm, 12,59%, 61,06%) dan memiliki spektrum inframerah yang paling sesuai dengan kolagen standar. Kolagen ini kemudian diaduk dengan kecepatan 1000 rpm selama 6 dan 8 jam untuk pengecilan ukuran. Kolagen dengan pengadukan 6 jam mempunyai ukuran partikel lebih kecil (1,34 µm) dibandingkan dengan pengadukan 8 jam (1,80 µm). Kolagen dengan ukuran 1,34 µm menunjukkan aktivitas terbaik yaitu aktivitas antioksidan terhadap 2,2-difenil-1-pikrilhidrazil (DPPH) sebesar 24,70% dan inhibitor tirosinase sebesar 26,77%. Berdasarkan aktivitas antioksidan, antiglikasi, dan antitirosinase, kolagen dengan perendaman NaOH 0,10 M dan pengadukan selama 6 jam memiliki sifat anti aging yang paling baik.</p><p><strong><strong>In Vitro Anti-Aging Activity of Chicken (<em>Gallus gallus domesticus</em>) Bone Waste Collagen</strong>. </strong>Chicken bone waste increases with increasing chicken compsumtion. However, study on utilizing chicken bone for collagen source has not been widely explored. This study aims to determine the effect of collagen size on their anti aging activity, and to obtain the optimum condition to produce the chicken (<em>Gallus gallus domesticus</em>) collagen in the high yield and the best activity. Collagen isolation was carried out in various NaOH concentrations of 0.05 M, 0.10 M, and 0.20 M, followed by the maceration on acetic acid 1 M. The isolation in NaOH 0.10 M produced the collagen with particle size of 2.34 µm in yield of 12.59% and anti-glycation of 61.06%. The revealed infrared spectrum of the isolated collagen is almost the same with the spectrum of the standart collagen. The collagen in 2.34 µm was further stirred at a 1000 rpm for 6 and 8 hours to reduce the size. Collagen stirred in 6 hours has a smaller particle size (1.34 µm) compared with that of stirred in 8 hours which has a particle size of 1.80 µm. The collagen with size of 1.34 µm showed the best activity, which revealed the antioxidant activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) of 24.70% and tyrosinase inhibitors of 26.77%. Based on antioxidant activity, anti-glycation, and anti-tyrosinase, the collagen which was isolated in 0.10 M NaOH and was stirred in 6 hours has the best anti-aging property.</p>

The Combination of Turmeric (Curcuma domestica) Rhizome Extract and Collagen in A Serum Formulation as an Antioxidant

“…The addition of collagen aims to help prevent premature aging because collagen had anti-aging activity. Collagen with a smaller size would have a higher antioxidant activity 16 .…”

“…Research on collagen has been carried out by Budiarti16 by testing collagen from chicken bone waste (Gallus gallus domesticus) against anti-aging activity in vitro, where the results obtained by stirring for six hours have a smaller particle size(1.34 µm) compared to eight hours (1.80 µm) stirring. Collagen with a size of 1.34 µm showed the best activity, namely antioxidant activity against 2,2diphenyl-1-picrylhydrazyl (DPPH) of 24.7% and tyrosinase inhibitor of 26.77%.…”

Survey of Sexual Harassment to Students at Indonesia University

“…In fact, the majority of female victims in Indonesia experience victim blame. Women who are victims of sexual violence are considered to be the cause of sexual violence (Budiarti;Arsa Ilmi et al, 2022;Supriyanto, 2023). Various reasons such as being at the wrong time and place, not fighting back, or the woman herself inviting others to commit a crime are blamed on the female victim.…”

“…Various reasons such as being at the wrong time and place, not fighting back, or the woman herself inviting others to commit a crime are blamed on the female victim. A high sense of ownership, forcing women to be able to accept all treatments and actions from their owners (men) (Budiarti;Arsa Ilmi et al, 2022). Thus, empathy only favors men, even though they are the perpetrators of sexual harassment (Bongiorno et al, 2020).…”

Review Artikel: Pemanfaatan Limbah Tulang Ayam Sebagai Sumber Kolagen Untuk Anti Aging Pada Kulit

“…Penelitian Budiarti et al (2019) terhadap tulang ayam broiler menyatakan kandungan protein sebesar 51,73% dan lemak 15,23%. Kadar protein pada sampel tulang tersebut menentukan jumlah kolagen yang terkandung dan memiliki potensi yang baik untuk dijadikan sebagai sumber kolagen.…”