Kompartimentierung bei den Enzymkaskaden: statisch oder Durchfluss

Abstract

Development of efficient enzymatic reaction cascades necessitates individual and careful handling of reaction conditions, which may differ from one enzymatic step to another one. Compartmentalization is a key solution that can be either done under static or flow conditions. The recent developments enable the use of different strategies, materials and set-ups, which can be applied based on the requirements of different bioprocesses.

The rise of continuous flow biocatalysis – fundamentals, very recent developments and future perspectives

“…Eutectic mixtures are obtained mixing Lewis or Brønsted acids and bases containing a variety of anionic and/or cationic species. [255][256][257] However, the final solvent has a lower intrinsic toxicity compared to ionic liquids, making them to promising substitutes. 258 Its main disadvantage is the high viscosity (which depends on the starting materials) being a problem for the scale-up of the process, but it can be easily overcome by mixing them with other (co)solvents.…”