Komplexbildner als Cofaktoren isolierter Zellgranula

Raaflaub, J

doi:10.1002/hlca.19550380106

Cited by 78 publications

(58 citation statements)

References 12 publications

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“…Light-scattering: The optical density at 520 my decreased linearly with increasing sucrose or glucose concentrations, in contrast to what is commonly found in mitochondria (24). Changes in optical density are not necessarily an indication of volume change (28) but may also be due to changes in the refractive indices of either the particles or the dispersing medium (3).…”

Section: Chemical Analysis Of Washed Native-fatmentioning

confidence: 97%

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Fat Metabolism in Germinating Soybeans. I. Physiology of Native Fat

Kahn

Howell²,

Hanson³

1960

Plant Physiol.

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Section: Chemical Analysis Of Washed Native-fatmentioning

confidence: 97%

“…Weights were corrected for the occluded sucrose by the method of Trevelyan and Harrison (30). B: Light-scattering was measured spectrophotometrically at 520 m/A by the methods of Cleland (6) and Raaflaub (24). C: Viscosity was determined after dialysis in the cold to equilibrium against various sucrose concentrations.…”

mentioning

confidence: 99%

Fat Metabolism in Germinating Soybeans. I. Physiology of Native Fat

Kahn

Howell²,

Hanson³

1960

Plant Physiol.

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“…Additional evidence for the above mentioned interpretations came from observations on changes in low amplitude light scattering and optical density (12-16), which accompanied the "energy states" described by Chance and Williams (17) . Reports (18)(19)(20)(21)(22)(23) appeared previously which showed that energy transduction in the mitochondrion included not only oxidative phosphorylation and active transport but also mechanochemical processes . Other mechanochemical studies have shown that energy derived from ATP (24) or from electron transport (25,26) can drive mitochondrial contraction .…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural Studies of Beef Heart Mitochondria

Weber

1972

The Journal of Cell Biology

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The relationships between membranes and intramembrane compartments of isolated heart mitochondria are inadequately defined to express the induced morphological changes associated with the structural organization. The inner membrane and matrix are the major structural entities which undergo transformation upon alteration of metabolism or incubation conditions. To better express these morphological changes within a mitochondrion, two inner membranes plus enclosed matrix are defined as an inmerix (plural inmerices). Three general morphological forms of mitochondria can be distinguished by the size and shape of inmerices. These are distended, condensed, and coalesced inmerixal configurations. Hypotonic conditions and Pi in isotonic sucrose generate distended configurations. This Pi distention is apparently dependent on utilization of energy. It does not occur under anaerobic conditions. Oxidizable substrates generate condensed configurations. ADP and dADP generate coalesced configurations and stop formation of condensed and distended inmerixal configurations in the absence of inhibitors. ADP coalescence is apparently not dependent on an energy input. It occurs under aerobic and anaerobic conditions, and in isotonic and hypotonic media. Atractyloside completely inhibits the effects of ADP on inmerixal membranes whereas oligomycin does not. Distention by Pi is unaffected by the two inhibitors. Distended inmerices, without added Pi (12 mM and 62 mM sucrose), are coalesced by ADP. These studies indicate that coalescence of inmerixal membranes probably reflects the consequences of specific stoichiometric binding or translocation of adenine nucleotides.

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“…The swelling of isolated mitochondria in response to the introduction of orthophosphate (Pi) to the suspending medium has been studied in a number of works (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15) . In particular, Packer (11) found the P ; induced swelling to be dependent on the presence of state 4 and to be reversed upon the induction of state 3 .…”

Section: Introductionmentioning

confidence: 99%