Availability of quality and effective tiger barb seeds is highly limited and leads to unfulfilled market demand. This study was conducted from April - May 2018 in the Laboratory of Aquaculture, Faculty of Agriculture, University of Asahan. Result of analysis of variance (ANOVA) shows that Fstatistic≫Ftable (636,097≫0,17), thus H0 is rejected and H1 is accepted, hence treatment of different substrates significantly affected egg number of tiger barb. Similarly, the value of fecundity, hatching rate, and survival rate obtained in this study resulted in Fstatistic≫Ftable (353.676≫0,17) or H0 is rejected and H1 is accepted, hence significant effect on hatching rate was obtained due to substrate treatment and Fstatistic≫Ftable (38,741≫0,17) H0 is rejected and H1 is accepted which reflected significant influence on tiger barb survival by reason of different substrate treatment. Hatching Rate is obtained by calculating the number of eggs hatched by tiger barbs (Puntiustetrazona). The highest hatching rate of tiger barb in this study was observed in treatment of Hydrillasp (88,14%) followed by palm fiber (72,89%) and water hyacinth (72,75%), while the lowest percentage was obtained by treatment of twigs and raffia rope.