South Sulawesi province is among the Indonesia rice barns, which is expected to buffer the national rice supply. Although rice stock is mainly produced from high-yielding superior varieties, local germplasm is still the main basis for variety development. Local germplasm has been adapted to various agro-ecosystems and thus has potential as a genetic source for adaptation to climate change. This study was aimed at measuring the diversity of rice germplasm from South Sulawesi based on its grain characteristics. A total of 98 rice accessions that were conserved in the agricultural genebank were examined. Grain length, shape, color, and other grain-related characters were observed following the international standard descriptors for rice. NTSYS-PC software version 2.2 was used to perform clustering analysis. Rice collections from South Sulawesi showed diversity in their grain characteristics. The majority of seeds are awnless, have medium length, medium shape, and straw-colored lemmae-palea and attributed with medium-sized and straw-colored sterile lemmae, and the dehulled grain bran is predominantly white. The rice accessions can be grouped into eight clusters at a similarity level of 52%. This diversity and similarity information is important for germplasm management and utilization. However, due to the limited number of characters used, information from this study cannot yet be used as a basis for their management. More comprehensive characterization efforts need to be conducted in order to make this study more usable, both in supporting conservation and for their utilization