Konzentration und Turnover der energiereichen Phosphate des Herzens nach Studien mit Papierchromatographie und Radiophosphor

Fleckenstein, A.; Janke, J.; Gerlach, E.; Deuticke, Mitarbeit von B.

doi:10.1007/bf01483774

Cited by 59 publications

(7 citation statements)

References 17 publications

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“…an immediate decrease in CP and ATP and an increase in lactate and a-GP content of ischemic muscle. The observations are in line with those reported previously (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15) and reflect the diminished oxidative metabolism but increased glycolytic or anaerobic metabolism of the ischemic muscle.…”

Section: Discussionsupporting

confidence: 92%

Early Changes in Energy Metabolism in the Myocardium Following Acute Coronary Artery Occlusion in Anesthetized Dogs

Braasch

Gudbjarnason

Puri

et al. 1968

Circulation Research

232

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The tissue content of energy-rich phosphates and glycolytic metabolites and the activity of myocardial enzymes were examined in the dog after producing myocardial infarction by ligating branches of the anterior descending and circumflex coronary arteries. The pattern of systolic fiber movement shown by a strain-gauge assembly differentiated ischemic from nonischemic portions of myocardium. In ischemic muscle, 30 minutes after onset of ischemia, creatine phosphate content fell from 8.0 to 1.4 / z moles/g, ATP content fell from 5.8 to 1.5 fxmo]es/g, lactate content rose tenfold, and a-glycerophosphate content rose fivefold. The content of energy-rich phosphates and glycolytic metabolites did not change much in nonischemic muscle. The activities of myocardial enzymes were assayed in extracts of tissue samples from ischemic and nonischemic muscle 5 to 120 minutes after coronary artery occlusion. The activities of aldolase, lactic dehydrogenase (LDH), glyceraldehydephosphate dehydrogenase, a-glycerophosphate dehydrogenase, malatedehydrogenase (MDH).and 6-phosphogluconate dehydrogenase did not change significantly in ischemic muscle during 2 hours of observation. In nonischemic muscle, phosphofr uctokinase activity increased 75% 5 minutes after coronary occlusion, followed by an increase in activity of isocitrate dehydrogenase, creatinephosphokinase, MDH, and LDH. The enzymatic changes in nonischemic muscle suggest metabolic changes in nonischemic muscle accompanying compensatory hyperfunction and increased energy requirements of surviving muscle. ADDITIONAL KEY WORDSmyocardial ischemia enzyme profile glycolysis energy-rich phosphates myocardial fiber shortening paradoxical systolic fiber lengthening • Previous communications from this laboratory have described the biochemical changes in heart muscle that occur from 5 hours to 10 weeks following coronary oc-

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Section: Discussionsupporting

confidence: 92%

Early Changes in Energy Metabolism in the Myocardium Following Acute Coronary Artery Occlusion in Anesthetized Dogs

Braasch

Gudbjarnason

Puri

et al. 1968

Circulation Research

232

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“…The levels of CP we found in perfused hearts are lower than previously reported invivo levels (25). However, the levels of ATP we found are within normal limits for the rat (25,26).…”

Section: Discussionsupporting

confidence: 45%

“…However, the levels of ATP we found are within normal limits for the rat (25,26). Since ATP is considered to be the direct energy donor for contractile and electrical events (4,10), and CP is considered to serve a reservoir function, the relation of ATP levels to the ECG and mechanical changes should not be affected by a modest reduction in CP.…”

Section: Discussionmentioning

confidence: 45%

Effects of High-Energy Phosphate Depletion and Repletion on the Dynamics and Electrocardiogram of Isolated Rat Hearts

Scheuer

Stezoski

1968

Circulation Research

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To explore the sequence of metabolic, mechanical, and electrocardiographic (ECG) events during myocardial anoxia, isolated rat hearts were paced from the atrium. Anoxic and recovery periods were studied. Adenosine triphosphate (ATP) and creatine phosphate (CP) declined to 50% of control during the first minute and remained at that level for the 5-minute anoxic period. ATP and CP returned to control values after 10 and 20 seconds of recovery. Lactate and potassium efflux from the myocardium closely followed the highenergy phosphate changes. During anoxia, left ventricular systolic pressure increased initially, then fell below the control level after 2 minutes, and recovered within 20 seconds of reoxygenation. In catecholamine-depleted hearts, it fell immediately with anoxia, and recovery was incomplete. The conduction time for the pacing stimulus to reach the ventricle increased with anoxia and decreased with reoxygenation. S-T alterations in the ECG also lagged behind high-energy phosphate reduction and recovery.The study demonstrates that in the isolated heart, ECG evidence of myocardial hypoxia may be absent when high-energy phosphate levels in the myocardium are very low. Mechanical changes are more closely related temporally to high-energy phosphate alterations than are ECG changes. The release of endogenous catecholamines is important to maintain mechanical function in the hypoxic heart,

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“…(5) To explain the unknown resonance lines, the spectra of the heart and skeletal muscle were compared with that of an adenosine tetraphosphate solution. After separation of Mg ++ ions from ATP at low intracellular pH values the resonance lines shifted by some 1-4 ppm.…”

Section: Study For Clarifying Two Unknown Peaks In the Phosphorus Magmentioning

confidence: 99%

“…Wurde aber in diesen frtiheren Versuchen eine Verlaufsanalyse des Gehaltes an energiereichen Phosphaten beabsichtigt, so mugten dazu mehrere Tiere nacheinander in verschiedenem zeitlichen Abstand get6tet werden [5,13]. Wurde aber in diesen frtiheren Versuchen eine Verlaufsanalyse des Gehaltes an energiereichen Phosphaten beabsichtigt, so mugten dazu mehrere Tiere nacheinander in verschiedenem zeitlichen Abstand get6tet werden [5,13].…”

Section: Study For Clarifying Two Unknown Peaks In the Phosphorus Magunclassified

Ein Beitrag zur Aufklärung von zwei unbekannten31P Resonanzen im Kernspinresonanzspektrum während des Zerfalls energiereicher Phosphatverbindungen in ischämischer Herz- und Skelettmuskulatur

Keidel

Reiman

et al. 1984

Res. Exp. Med.

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Using the 31-Phosphorus pulsed NMR-technique by FFT information processing it was possible to obtain the following results: The phosphorus compounds were measured as time function in the course of 24 h in isolated skeletal and cardiac muscle of rana temporaria in a dynamic NMR experiment. The shift of the observed resonance lines by increasing intracellular H+ concentration during anoxic conditions will be described in this paper in accordance with the NMR literature. The time course of the beta group of ATP (which is representative for ATP) has been found not to decrease monotonically but rather periodically in a half-hour periodicity until finally a complete fading of ATP in the NMR spectrogram occurs. Besides the resonances described in the literature it was possible to record two additional unknown resonance lines 1.6 and 4.6 ppm apart from the classical alpha- and beta ATP peaks. The peak area of this up to now unknown substance followed a periodic law, regarding the first 2-3 h after preparation. Afterward the concentration dropped to zero. Some shift of the resonance lines of the NMR spectrum could be observed dependent upon the fact that in the muscle cell the ATP normally occurs as a complex with Mg++ ions (MgATP). After separation of Mg++ ions from ATP at low intracellular pH values the resonance lines shifted by some 1-4 ppm. This was tested by test solutions containing MgATP as a complex of free ATP. Although this observation could not sufficiently explain the two as yet unknown lines in the NMR spectra, this result gave rise to further exploration of the phenomenon. To explain the unknown resonance lines, the spectra of the heart and skeletal muscle were compared with that of an adenosine tetraphosphate solution. Based upon the fact that the frequencies of the lines under discussion are identical with those of the adenosine tetraphosphate, one can conclude with a high degree of probability that the as yet unknown lines are originated by adenosine tetraphosphate. If this hypothesis is correct, the concentration of adenosine tetraphosphate could be determined in the order of 1 mM, thus being one third or one quarter of the concentration of the adenosine triphosphate. To give better evidence for the clarification of the newly found substance it will be necessary to record under identical experimental conditions 1H and 13C NMR spectra during the time course of deterioration of the skeletal and cardiac muscle.(ABSTRACT TRUNCATED AT 400 WORDS)

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Konzentration und Turnover der energiereichen Phosphate des Herzens nach Studien mit Papierchromatographie und Radiophosphor

Cited by 59 publications

References 17 publications

Early Changes in Energy Metabolism in the Myocardium Following Acute Coronary Artery Occlusion in Anesthetized Dogs

Early Changes in Energy Metabolism in the Myocardium Following Acute Coronary Artery Occlusion in Anesthetized Dogs

Effects of High-Energy Phosphate Depletion and Repletion on the Dynamics and Electrocardiogram of Isolated Rat Hearts

Ein Beitrag zur Aufklärung von zwei unbekannten31P Resonanzen im Kernspinresonanzspektrum während des Zerfalls energiereicher Phosphatverbindungen in ischämischer Herz- und Skelettmuskulatur

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