Korean Red Ginseng water extract arrests growth of xenografted lymphoma cells

Park, Jae Gwang; Son, Young–Jin; Aravinthan, Adithan; Kim, Jong-Hoon; Cho, Jae Youl

doi:10.1016/j.jgr.2016.07.006

Cited by 31 publications

(20 citation statements)

References 42 publications

(50 reference statements)

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“…A xenograft mouse model was generated as reported previously [ 50 , 51 ]. Briefly, CT26 cells (10 4 cells in 0.1 ml medium) were injected subcutaneously using a 27-gauge needle.…”

Section: Methodsmentioning

confidence: 99%

Src is the primary target of aripiprazole, an atypical antipsychotic drug, in its anti-tumor action

et al. 2017

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Aripiprazole (ARP) is an atypical anti-psychotic drug widely used to treat schizophrenia and bipolar disorder. The pharmacological effects of ARP on cancer cells are still poorly understood. In this study, anti-cancer effects of ARP on various malignant tumor cells and its molecular mechanism were further carefully examined by using cell proliferation assay, xenograft mouse model, immunoblotting analysis, migration assay, luciferase reporter gene assay, kinase assay, and overexpression strategy. Treatment with ARP induced cytotoxicity in U251 glioma cells, MKN-1 gastric adenosquamous carcinoma cells, and CT26 colon carcinoma cells. ARP suppressed cell proliferation of LN428, MDA-MB-231, and HEK293 cells. Pro-apoptotic factors active caspase-3, -8, and -9, as well as p53, were upregulated, whereas the protein and mRNA levels of anti-apoptotic factor B-cell lymphoma 2 (Bcl-2) decreased. In agreement with the in vitro results, ARP compound also significantly suppressed the growth of tumor masses formed by injecting CT26 colon cancer cells into mice. ARP treatment also effectively decreased the migratory ability of U251 glioma cells by downregulating metalloproteinase-9. Levels of phosphorylated Src, phosphorylated phosphatidylinositide 3-kinase (PI3K), and phosphorylated signal transducer and activator of transcription 3 (STAT3) were significantly decreased following ARP treatment. ARP compound reduced the kinase activity of Src. Our studies suggest that Src may be an important target molecule linked to the antitumor effects of ARP.

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“…A xenograft mouse model was generated as reported previously [ 50 , 51 ]. Briefly, CT26 cells (10 4 cells in 0.1 ml medium) were injected subcutaneously using a 27-gauge needle.…”

Section: Methodsmentioning

confidence: 99%

Src is the primary target of aripiprazole, an atypical antipsychotic drug, in its anti-tumor action

et al. 2017

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“…The content of ginsenosides in the ginseng calyx extract was determined using the HPLC-UV method, as reported previously [38] , [39] . The mobile phase consisted of a mixture of acetonitrile (A) and water (B).…”

Section: Methodsmentioning

confidence: 99%

AKT-targeted anti-inflammatory activity of Panax ginseng calyx ethanolic extract

Han

Kim

et al. 2018

Journal of Ginseng Research

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BackgroundKorean ginseng (Panax ginseng) plays an anti-inflammatory role in a variety of inflammatory diseases such as gastritis, hepatitis, and colitis. However, inflammation-regulatory activity of the calyx of the P. ginseng berry has not been thoroughly evaluated. To understand whether the calyx portion of the P. ginseng berry is able to ameliorate inflammatory processes, an ethanolic extract of P. ginseng berry calyx (Pg-C-EE) was prepared, and lipopolysaccharide-activated macrophages and HEK293 cells transfected with inflammation-regulatory proteins were used to test the anti-inflammatory action of Pg-C-EE.MethodsThe ginsenoside contents of Pg-C-EE were analyzed by HPLC. Suppressive activity of Pg-C-EE on NO production, inflammatory gene expression, transcriptional activation, and inflammation signaling events were examined using the Griess assay, reverse transcription-polymerization chain reaction, luciferase activity reporter gene assay, and immunoblotting analysis.ResultsPg-C-EE reduced NO production and diminished mRNA expression of inflammatory genes such as cyclooxygenase-2, inducible NO synthase, and tumor necrosis factor-α in a dose-dependent manner. This extract suppressed luciferase activity induced only by nuclear factor-κB. Interestingly, immunoblotting analysis results demonstrated that Pg-C-EE reduced the activities of protein kinase B (AKT)1 and AKT2.ConclusionThese results suggest that Pg-C-EE may have nuclear-factor-κB-targeted anti-inflammatory properties through suppression of AKT. The calyx of the P. ginseng berry is an underused part of the ginseng plant, and development of calyx-derived extracts may be useful for treatment of inflammatory diseases.

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“…The cytotoxic effects of Ta-EE on RAW264.7 cells (1 × 10 6 cells/ml) and SW1353 cells (1 × 10 6 cells/ml) were determined by treating the cells with escalating doses of Ta-EE (0, 75, 100, and 300 μ g/ml) for 24 h, and cell viability was determined by MTT assay as reported previously [ 50 , 51 ]. Briefly, the cell culture media were mixed with MTT solution (10 mg/ml in PBS, pH 7.4) and incubated at 37°C for 4 h. A 15% SDS solution was directly added to the mixture to stop the reaction followed by incubation at 37°C for 24 h. Optical density was then measured at 570 nm using a Spectramax 250 microplate reader.…”

Section: Methodsmentioning

confidence: 99%

Tabetri™ (Tabebuia avellanedae Ethanol Extract) Ameliorates Osteoarthritis Symptoms Induced by Monoiodoacetate through Its Anti-Inflammatory and Chondroprotective Activities

Park

Hong

et al. 2017

Mediators of Inflammation

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Although osteoarthritis (OA), a degenerative joint disease characterized by the degradation of joint articular cartilage and subchondral bones, is generally regarded as a degenerative rather than inflammatory disease, recent studies have indicated the involvement of inflammation in OA pathogenesis. Tabebuia avellanedae has long been used to treat various diseases; however, its role in inflammatory response and the underlying molecular mechanisms remain poorly understood. In this study, the pharmacological effects of Tabetri (Tabebuia avellanedae ethanol extract (Ta-EE)) on OA pathogenesis induced by monoiodoacetate (MIA) and the underlying mechanisms were investigated using experiments with a rat model and in vitro cellular models. In the animal model, Ta-EE significantly ameliorated OA symptoms and reduced the serum levels of inflammatory mediators and proinflammatory cytokines without any toxicity. The anti-inflammatory activity of Ta-EE was further confirmed in a macrophage-like cell line (RAW264.7). Ta-EE dramatically suppressed the production and mRNA expressions of inflammatory mediators and proinflammatory cytokines in lipopolysaccharide-stimulated RAW264.7 cells without any cytotoxicity. Finally, the chondroprotective effect of Ta-EE was examined in a chondrosarcoma cell line (SW1353). Ta-EE markedly suppressed the mRNA expression of matrix metalloproteinase genes. The anti-inflammatory and chondroprotective activities of Ta-EE were attributed to the targeting of the nuclear factor-kappa B (NF-κB) and activator protein-1 (AP-1) signaling pathways in macrophages and chondrocytes.

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Korean Red Ginseng water extract arrests growth of xenografted lymphoma cells

Cited by 31 publications

References 42 publications

Src is the primary target of aripiprazole, an atypical antipsychotic drug, in its anti-tumor action

Src is the primary target of aripiprazole, an atypical antipsychotic drug, in its anti-tumor action

AKT-targeted anti-inflammatory activity of Panax ginseng calyx ethanolic extract

Tabetri™ (Tabebuia avellanedae Ethanol Extract) Ameliorates Osteoarthritis Symptoms Induced by Monoiodoacetate through Its Anti-Inflammatory and Chondroprotective Activities

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