KRAS and VEGF gene 3'-UTR single nucleotide polymorphisms predicted susceptibility in colorectal cancer

Yang, Mu; Xiao, Xiuli; Xin, Xing; Li, Xin; Xia, Tian; Long, Hu

doi:10.1371/journal.pone.0174140

Cited by 10 publications

(8 citation statements)

References 40 publications

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“…Another study reported contradictive results. Yang et al did not find any significant relationship between VEGF gene polymorphisms rs3025039C/T, rs3025040C/T, rs3025053G/A, and rs10434A/G with risk of colorectal cancer in the Han ethnic [43].…”

Section: Discussionmentioning

confidence: 96%

Association between VEGF Gene Polymorphism -634G>C and Risk of Colorectal Cancer

Tambunan

Siregar

Syam

2022

Open Access Maced J Med Sci

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BACKGROUND: Genetic and environmental factors play an important role in the pathogenesis of colorectal cancer. Angiogenesis is a central process in carcinogenesis and is affected by vascular endothelial growth factor (VEGF). Several genetic variations, such as polymorphism, may alter VEGF expression and influence the risk of colorectal cancer. AIM: The objective of this study was to determine the association between VEGF gene polymorphism -634G>C and risk of colorectal cancer. METHODS: A cross-sectional study was conducted at Haji Adam Malik general hospital and its sister hospitals. Subjects were obtained by consecutive sampling. Inclusion criteria for case and control groups were patients with colorectal cancer and healthy subject, respectively, aged 18 years or older and willing to participate in the study. Exclusion criteria were patients with systemic comorbidities or malignancies in other organs. Each subject undergoes an interview, colonoscopy, biopsy, serum VEGF level measurement, and VEGF polymorphism -634G>C evaluation. RESULTS: Eighty subjects were enrolled and distributed into case and control groups. Males were dominant in both groups, with a mean age of older than 55 years. Most lesions were in the rectum and 45% of subjects had moderately differentiated cancer. The median serum VEGF level in the case group was higher than the control group (2,175.1 pg/mL vs. 253 pg/mL; p < 0.001). VEGF gene polymorphism -634G > C was associated with the risk of colorectal cancer (p = 0.048). GG and GC genotypes had 1.89 times higher risk of colorectal cancer compared to the CC genotype. GG genotype and G allele had the highest serum VEGF levels. CONCLUSION: There is an association between VEGF gene polymorphism -634G>C and risk of colorectal cancer in this study.

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Section: Discussionmentioning

confidence: 96%

Association between VEGF Gene Polymorphism -634G>C and Risk of Colorectal Cancer

Tambunan

Siregar

Syam

2022

Open Access Maced J Med Sci

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“…This gene affects angiogenesis, tumor growth, and metastasis . It is also related to CRC outcomes and treatment . Thus the association between rs3025039 and CRC risk may be related to the effect of this SNP on miRNA:mRNA interactions.…”

Section: Discussionmentioning

confidence: 99%

“…187 It is also related to CRC outcomes and treatment. 124 Thus the association between rs3025039 and CRC risk may be related to the effect of this SNP on miRNA:mRNA interactions. However, in the previous meta-analysis with five included studies, no significant association was found between this polymorphism and risk of CRC.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‐binding site polymorphisms and risk of colorectal cancer: A systematic review and meta‐analysis

et al. 2019

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Genetic variations in miRNAs binding site might participate in cancer risk. This study aimed to systematically review the association between miRNA‐binding site polymorphisms and colorectal cancer (CRC). Electronic literature search was carried out on PubMed, Web of Science (WOS), Scopus, and Embase. All types of observational studies till 30 November 2018 were included. Overall 85 studies (21 SNPs) from two systematic searches were included analysis. The results showed that in the Middle East population, the minor allele of rs731236 was associated with decreased risk of CRC (heterozygote model: 0.76 [0.61‐0.95]). The minor allele of rs3025039 was related to increased risk of CRC in East Asian population (allelic model: 1.25 [1.01‐1.54]). Results for rs3212986 were significant in overall and subgroup analysis (P < .05). For rs1801157 in subgroup analysis the association was significant in Asian populations (including allelic model: 2.28 [1.11‐4.69]). For rs712, subgroup analysis revealed a significant (allelic model: 1.41 [1.23‐1.61]) and borderline (allelic model: 0.92 [0.84‐1.00]) association in Chinese and Czech populations, respectively. The minor allele of rs17281995 increased risk of CRC in different genetic models (P < .05). Finally, rs5275, rs4648298, and rs61764370 did not show significant associations. In conclusion, minor allele of rs3025039, rs3212986, and rs712 polymorphisms increases the risk of CRC in the East Asian population, and heterozygote model of rs731236 polymorphism shows protective effect in the Middle East population. In Europeans, the minor allele of rs17281995 may increase the risk of CRC, while rs712 may have a protective effect. Further analysis based on population stratifications should be considered in future studies.

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“…Identification of a target in a complex biological matrix prepared with human urine was demonstrated. Because the discrimination of a single nucleotide mutation is important for a number of genetic diseases, this nanotechnology can be instrumental in detection of SNPs beyond miRNA research …”

Section: Discussionmentioning

confidence: 99%

Homologous miRNA Analyses Using a Combinatorial Nanosensor Array with Two-Dimensional Nanoparticles

2018

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A novel combinatorial nanosensor array for miRNA analyses was assembled using the intrinsic noncovalent interactions of unmodified two-dimensional nanoparticles. Discrimination of nine miRNA analogues with as little as a single nucleotide difference was demonstrated under 2 h. All nine targets were identified simultaneously with 95% confidence. The developed nanotechnology offered identification and quantification of unknown targets with unknown concentration. Discrimination of target mixtures from low-to-high ratios was demonstrated. The DNA and RNA analogues of targets were identified using the combinatorial sensory approach. Identification of a target in a complex biological matrix prepared with human urine was demonstrated. The nanosensor array was put together using 15 nanoassemblies (2D-NAs) constructed using three two-dimensional nanoparticles (2D-nps: WS, MoS, and nanographene oxide (nGO)) and five rationally designed fluorescently labeled 15-nt-long ssDNAs (probes). In this approach, each target has only a small yet varying degree of complementarity with each of the five probes adsorbed on the 2D-np surface. The probes in each 2D-NA are desorbed from the surface by each target with a different degree that was recorded with fluorescence recovery measurements. The fluorescence data set was processed by partial least squares discriminant analysis (PLSDA), and each target was discriminated successfully. This new approach has a number of advantages over the classical bind-and-release model, typically used for 2D-np based biosensors, and opens greater detection opportunities with 2D-nps.

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KRAS and VEGF gene 3'-UTR single nucleotide polymorphisms predicted susceptibility in colorectal cancer

Cited by 10 publications

References 40 publications

Association between VEGF Gene Polymorphism -634G>C and Risk of Colorectal Cancer

Association between VEGF Gene Polymorphism -634G>C and Risk of Colorectal Cancer

MicroRNA‐binding site polymorphisms and risk of colorectal cancer: A systematic review and meta‐analysis

Homologous miRNA Analyses Using a Combinatorial Nanosensor Array with Two-Dimensional Nanoparticles

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