Kribbella catacumbae sp. nov. and Kribbella sancticallisti sp. nov., isolated from whitish-grey patinas in the catacombs of St Callistus in Rome, Italy

Urzı̀, Clara; Leo, Filomena De; Schümann, Peter

doi:10.1099/ijs.0.65613-0

Cited by 42 publications

(14 citation statements)

References 41 publications

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“…Two new species of the genus Kribbella, namely K. catacumbae and K. sancticallisti were described from this site (Urzì et al, 2008). Among the Gram negative (15.2% of the total of isolated strains), Pseudomonas was the most common genus found (6.8%).…”

Section: St Callistus Catacombsmentioning

confidence: 99%

“…Underlined strains were those identified by sequencing of 16S rDNA; (*) data taken from Urzì et al, 2010. (^) strains described by two of the Authors (Urzì et al, 2008). …”

Section: Streptomyces Badiusmentioning

confidence: 99%

“…Furthermore, through the isolation of bacterial strains, it becomes possible to discover strains with interesting taxonomies (Groth et al, 2005(Groth et al, , 2006Jurado et al, 2005aJurado et al, , 2005bUrzì et al 2008;Zammit et al, 2010Zammit et al, , 2011a, and also strains with biotechnological potential including applications in art conservation.…”

Section: Streptomyces Badiusmentioning

confidence: 99%

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Chemoorganotrophic bacteria isolated from biodeteriorated surfaces in cave and catacombs

Leo¹,

Iero²,

Zammit³

et al. 2012

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The main objective of this work was the comparative analysis of a large number of bacterial strains isolated from biodeteriorated surfaces in three different sites, namely the catacombs of St. Callistus in Rome, Italy, the catacombs dedicated to St. Agatha in Rabat, Malta and the Cave of Bats in Zuheros, Spain. Our results showed that even considering only culturable chemoorganotrophic bacteria the variability is very high, reflecting the great variety of microhabitats present. Hence any strategies to prevent, control or eliminate the biofilm-embedded microbiota from an archeological surface should take into account a number of considerations as stipulated in our study.

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Section: St Callistus Catacombsmentioning

confidence: 99%

“…Underlined strains were those identified by sequencing of 16S rDNA; (*) data taken from Urzì et al, 2010. (^) strains described by two of the Authors (Urzì et al, 2008). …”

Section: Streptomyces Badiusmentioning

confidence: 99%

Section: Streptomyces Badiusmentioning

confidence: 99%

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Chemoorganotrophic bacteria isolated from biodeteriorated surfaces in cave and catacombs

Leo¹,

Iero²,

Zammit³

et al. 2012

IJS

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“…UTMC 267. Kribbella sancticallisti was first discovered as a whitish grey patina growing in Roman catacombs in 2008 (Urz ı et al 2008). One of the major achievements of the study was highlighting the therapeutic potential of members of the Kribbella sp.…”

Section: Discussionmentioning

confidence: 99%

Isolation and screening of rareActinobacteria, a new insight for finding natural products with antivascular calcification activity

et al. 2017

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Aim: Vascular calcification (VC) is a significant pathological process in some life-threatening diseases. Several pathological mechanisms, including transdifferentiation of vascular smooth muscle cells to osteoblast-like cells and apoptosis are involved in VC. Compounds with an inhibitory effect on these processes are potentially efficient medications. In consideration of the multiple biological activities of Actinobacteria, this research was aimed at finding anti-VC metabolite-producing Actinobacteria. Methods and Results: After the isolation and identification of Actinobacteria, the effect of their fermentation broth extracts on the apoptosis rate was measured using various methods, for example, ethidium bromide/acridine orange staining, DNA laddering and diphenylamine assays. The effect of the most effective fermentation broth extract of Actinobacteria (FBEA) on the mRNA expression of runt-related transcription factor 2 (Runx2) and osteopontin (OPN) was examined. Finally, the most effective FBEA was fractionated and the chemical composition of anti-VC fractions was analysed using GC-MS. Various VC inhibition rates were observed in the tested FBEA (20 lg ml À1 ; 17Á9-60Á15%). The inhibition of DNA fragmentation was 7-48%.The FBE with the greatest anticalcification activity belonged to Kribbella sp. UTMC 267 and, according to 16S rRNA analysis, Kribbella sancticallisti with a similarity of 98Á53% is its nearest neighbour. The FBE of Kribbella sp. UTMC 267 reduced Runx2 mRNA expression by 2Á95-fold and OPN mRNA expression by 28Á57-fold, both of which are considered significant (P < 0Á05). Finally, GC-MS analysis showed the existence of potent anti-oxidative and anti-inflammation agents in FBE of Kribbella sp. UTMC 267. Conclusions: Actinobacterial metabolites can provide a new strategy for treating VC diseases by reducing the expression of osteogenic genes, the apoptosis rate and oxidative stress. Significance and Impact of the Study: This study highlights the therapeutic potential of Kribbella sp. metabolites and Actinobacteria as a new natural source for drug discovery programs in the nonantibiotic bioactivity field.

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“…Kribbella catacumbae and Kribbella sancticallisti (Urzì et al, 2008) were isolated from the whitish-grey patina of Roman catacombs. Kribbella albertanoniae (Everest et al, 2013) and Kribbella italica (Everest et al, 2015) were from a biodeteriogenic biofilm sample in the Saint Callistus Roman catacomb.…”

mentioning

confidence: 99%

Kribbella pittospori sp. nov., an endophytic actinobacterium isolated from the surface-sterilized stem of an Australian native apricot tree, Pittosporum angustifolium

Kaewkla

Franco

2016

International Journal of Systematic and Evolutionary Microbiology

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An endophytic actinobacterium, strain PIP 158 T , was isolated from the stem of a native apricot tree (Pittosporum angustifolium) collected from the grounds of Flinders University, Adelaide, Australia. As a result of a polyphasic taxonomic study, this strain was identified as a member of the genus Kribbella. This strain was a Gram-stain-positive, aerobic actinobacterium with welldeveloped substrate mycelia which were non-motile and with hyphae fragmenting into short to elongated rod-like elements. Phylogenetic evaluation based on 16S rRNA gene sequence analysis placed this isolate in the family Nocardioidaceae, being most closely related to Kribbella sandramycini ATCC 39419 T and Kribbella albertanoniae BC640 T which share a similarity of 99.26 and 99.18 % with Kribbella hippodromi S1.4 T , respectively. Chemotaxonomic data including cell-wall components, major menaquinones and major fatty acids confirmed the affiliation of strain PIP 158 T to the genus Kribbella. The results of the phylogenetic analysis, including physiological and biochemical studies in combination with DNA-DNA hybridization, allowed the genotypic and phenotypic differentiation of strain PIP 158 T from the closest related species with validly published names. The name proposed for the novel species is Kribbella pittospori sp. nov. The type strain is PIP 158 T (=DSM 23717 T =NRRL B-24813 T ).The genus Kribbella belongs to the family Nocardioidaceae. Park et al. (1999) first described the genus Kribbella,with the description of Kribbella flavida and Kribbella sandramycini. This genus contains LL-diaminopimelic acid in the cell-wall peptidoglycan. The predominant menaquinone is MK-9 (H 4 ) and the fatty acid profile consists of large amounts of branched components. At the time of writing, the genus Kribbella comprised 23 recognized species which were recovered from different habitats. Most were isolated from soil: Kribbella koreensis (Sohn et al., 2003) et al., 2015) were from a biodeteriogenic biofilm sample in the Saint Callistus Roman catacomb. Kribbella aluminosa was isolated from a medieval alum slate mine (Carlsohn et al., 2007). Three recently described species were isolated from plant tissues: Kribbella solani from scab lesions of a potato tuber (Song et al., 2004), Kribbella lupini from the surface sterilized root of Lupinus angustifolius (Trujillo et al., 2006) and Kribbella endophytica from stem sample of a native apricot tree (Kaewkla & Franco, 2013a (Kimura, 1980). The Tamura-Nei model (Tamura & Nei, 1993) was applied in the maximum-likelihood analysis by using the SubtreePruning-Regrafting-Extensive (SPR level 5) program. The topology of the tree was evaluated by performing a bootstrap analysis (Felsenstein, 1985) based on 1000 replications.The phylogenetic evaluation showed clearly that strain PIP 158 T was a member of the genus Kribbella. The phylogenetic position with all members of the genus Kribbella was reconstructed and it showed that strain PIP 158 T was encompassed by other members of this genus (Fig. 1) Fig. 1 and Fig. ...

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Kribbella catacumbae sp. nov. and Kribbella sancticallisti sp. nov., isolated from whitish-grey patinas in the catacombs of St Callistus in Rome, Italy

Cited by 42 publications

References 41 publications

Chemoorganotrophic bacteria isolated from biodeteriorated surfaces in cave and catacombs

Chemoorganotrophic bacteria isolated from biodeteriorated surfaces in cave and catacombs

Isolation and screening of rareActinobacteria, a new insight for finding natural products with antivascular calcification activity

Kribbella pittospori sp. nov., an endophytic actinobacterium isolated from the surface-sterilized stem of an Australian native apricot tree, Pittosporum angustifolium

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