Kriopreservasi spermatozoa kambing Boer: Perbandingan dua bahan pengencer terhadap kualitas post-thawing dan kemampuan fertilisasinya

Pamungkas, Fitra Aji; Batubara, Aron; Anwar, M. S.

doi:10.14334/jitv.v19i2.1041

Cited by 4 publications

(11 citation statements)

References 26 publications

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“…The average percentage of motile and live spermatozoa in this study were 78.00% and 89.20% (Table 1). The average percentage of motile and live spermatozoa of Boer goats was 90.00% and 90.77% (Alawiyah and Hartono, 2006), 73.33% and 79.76% (Kostaman and Sutama, 2006), 88.00% and 89.67 (Hartono, 2010), 75% -93.33% and 96.08% -98.30% (Suharyati and Hartono, 2013), 79.55% and 85.29% (Pamungkas et al, 2014), and 89.00% and 87.00% (Rhochim et al, 2017).…”

Section: Characteristics Of Fresh Semen Of Boer Goatmentioning

confidence: 99%

“…Various results were reported about the abnormality of goat spermatozoa i.e. 4.33% (Alawiyah and Hartono, 2006), 7.30 -7.80% (Kostaman and Sutama, 2006), 1.98% (Hartono, 2010), 2.53% (Pamungkas et al, 2014) and 0.77% (Rhochim et al, 2017), whereas the percentage of intact plasma membrane was 77.25% (Pamungkas et al, 2014).…”

Section: Characteristics Of Fresh Semen Of Boer Goatmentioning

confidence: 99%

“…The results of the study showed that the average volume of fresh semen of Boer goat was 0.64 ml (Table 1). Several researchers have previously reported varying volumes of Boer goat semen, i.e., 0.69 -1.03 ml (Hastono et al, 2002), 1.49 ml (Alawiyah and Hartono, 2006), 0.60 -0.80 ml (Kostaman and Sutama, 2006), 0.53 ml (Mahmilia et al, 2006), 1.02 ml (Hartono, 2010), 0.77 to 1.13 ml (Suharyati and Hartono, 2013) 0.80 ml (Pamungkas et al, 2014), and 1.14 ml (Rhochim et al, 2017). The consistency, pH, and mass movement of Boer goat spermatozoa in this study were thick, 6.94, and +++, respectively ( Table 1).…”

Section: Characteristics Of Fresh Semen Of Boer Goatmentioning

confidence: 99%

“…The consistency, pH, and mass movement of Boer goat spermatozoa in this study were thick, 6.94, and +++, respectively ( Table 1). The characteristics of goat semen are thick consistency, pH between 6.40 to 7.25 and spermatozoa mass movement of +++ (Alawiyah and Hartono, 2006;Kostaman and Sutama, 2006;Pamungkas et al, 2014;Rhochim et al, 2017).…”

Section: Characteristics Of Fresh Semen Of Boer Goatmentioning

confidence: 99%

“…Based on the results of observation it was obtained that the average concentration of spermatozoa was 3,016 million cells/ml ( Table 1). The average concentration of Boer goat spermatozoa was 2,260 million cells/ml (Hastono et al, 2002), 5,154.75 million cells/ml (Alawiyah and Hartono, 2006), 2,700 -2,730 million cells/ml (Kostaman and Sutama, 2006), 2,290 million cells/ml (Hartono, 2010), 2,981 -3,568 million cells/ml (Suharyati and Hartono, 2013), 4,125 million cells/ml (Pamungkas et al, 2014), and 4,350.4 million cells/ml (Rhochim et al, 2017).…”

Section: Characteristics Of Fresh Semen Of Boer Goatmentioning

confidence: 99%

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The Quality of Boer Goat Semen Preserved with Sugar Palm Juice

Rizal¹,

Riyadhi²,

Sulaiman³

2018

BuletinPeternak

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The objective of this study was to examine the effect of seminal plasma on viability of Boer goat spermatozoa and effectiveness of sugar palm juice as an alternative extender during preservation at 5ºC. Semen of two Boer goats were collected using an artificial vagina. Fresh semen were evaluated and divided in equal volume into four tubes. Semen in the first and second tubes diluted with 80% sugar palm juice + 20% egg yolk (P1) and Andromed (P2), respectively. Semen in the third and fourth tubes were centrifuged with 3,000 RPM for 20 minutes, and the supernatant removed. diluted with 80% sugar palm juice + 20% egg yolk (P3) and Andromed (P4), respectively. Diluted-semen were preserved in refrigerator at 5oC, and quality of the spermatozoa including motile spermatozoa (MS), live spermatozoa (LS), and intact plasma membrane (IPM) were evaluated every day for four days. Results of this study showed that at day-2 preservation, mean percentages of MS, LS, and IPM for P2 (72, 83.4, and 83.4%), P3 (72, 82.6, and 82.2%), and P4 (72, 83, 83.8%) were significantly (P<0.05) higher than P1 (3, 24.8, and 25.2%). At day-3 preservation, mean percentages of MS, LS, and IPM for P2 (57, 65.6, and 69.6%) was significantly (P<0.05) higher than P3 (21, 34.8, and 31.8%), P4 (22, 33.6, and 31.2%), and P1 (0, 0, and 0%). In conclusion, semen of Boer goat to be preserved with extender containing egg yolk should be removed seminal plasma. Sugar palm juice containing egg yolk could be used as an extender for Boer goat semen, but should be applied in the AI program immediately after the semen is diluted.

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Section: Characteristics Of Fresh Semen Of Boer Goatmentioning

confidence: 99%

Section: Characteristics Of Fresh Semen Of Boer Goatmentioning

confidence: 99%

Section: Characteristics Of Fresh Semen Of Boer Goatmentioning

confidence: 99%

Section: Characteristics Of Fresh Semen Of Boer Goatmentioning

confidence: 99%

Section: Characteristics Of Fresh Semen Of Boer Goatmentioning

confidence: 99%

See 3 more Smart Citations

The Quality of Boer Goat Semen Preserved with Sugar Palm Juice

Rizal¹,

Riyadhi²,

Sulaiman³

2018

BuletinPeternak

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Influence of Sperm Number and Antioxidant Melatonin in Extender on the Quality of Post-Thawing Sheep Spermatozoa

Putri,

Karja,

Setiadi

et al. 2023

JITV

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This study aimed to examine the effect of spermatozoa concentration and the effectiveness of melatonin supplementation in diluent on the quality of post-thawing semen.Â Ejaculated semen was collected using the artificial vaginal method (MVB).Â The study was carried out in two stages, firstly semen was frozen in andromed diluent with different concentrations in one straw (50, 100, 200 million per straw), and the second was frozen semen in diluent supplemented with melatonin with different doses (0, 0,5, 1, 0 and 1.5 mM melatonin).Â Parameters observed were the movement of spermatozoa using Computer Assisted Sperm Analysis, membrane integrity, and acrosome integrity.Â Data were analyzed using ANOVA and further tested using Duncan's test.Â The results showed no significant difference in the quality and movement pattern of sheep semen when frozen at concentrations of 50, 100, or 200 million per straw (P 0.05).Â Furthermore, adding melatonin to the diluent in this study affected spermatozoa's total motility and progressive motility at a concentration of 1.0 mM (P0.05) but did not significantly affect the percentage of spermatozoa motility pattern characteristics.Â From the results, it can be concluded that the concentration of per straw spermatozoa does not affect the quality of sheep semen, and melatonin supplementation in diluent can reduce the effects of the frozen-thawed process on motility, acrosome cap, and plasma membrane integrity in sheep semen.Â Melatonin supplementation with a concentration of 1 mM in the extender was the highest quality concentration in this study.

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Pengaruh Berbagai Konsentrasi Laktosa Dan Sari Kedelai Pada Kualitas Semen Kambing Boer Yang Dipreservasi

M.A.,

NURSYAM,

RIYADHI

et al. 2024

MIP

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Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi laktosa dan sari kedelai untuk pengencer se- men kambing Boer. Metode penelitian menggunakan Rancangan Acak Lengkap (RAL), dengan empat perlakuan dan enam kali ulangan yang terdiri dari: 80% laktosa + 20% kuning telur sebagai kontrol (P0), 97% laktosa + 3% sari kedelai (P1), 95% laktosa + 5% sari kedelai (P2), dan 93% laktosa + 7% sari kedelai (P3). Semen kemudian di- simpan pada suhu 3-5oC dan diamati motilitas dan persentase spermatozoa hidup setiap hari selama empat hari. Hasil penelitian yang diperoleh menunjukkan bahwa konsentrasi sari kedelai berpengaruh sangat nyata (P<0,05) terhadap persentase motilitas dan spermatozoa hidup kambing Boer setelah pendinginan pada hari ketiga. Konsen- trasi sari kedelai 3% yang dikombinasikan dengan pengencer laktosa 97% menghasilkan persentase motilitas dan persentase hidup spermatozoa kambing Boer yang lebih baik.

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Kriopreservasi spermatozoa kambing Boer: Perbandingan dua bahan pengencer terhadap kualitas post-thawing dan kemampuan fertilisasinya

Cited by 4 publications

References 26 publications

The Quality of Boer Goat Semen Preserved with Sugar Palm Juice

The Quality of Boer Goat Semen Preserved with Sugar Palm Juice

Influence of Sperm Number and Antioxidant Melatonin in Extender on the Quality of Post-Thawing Sheep Spermatozoa

Pengaruh Berbagai Konsentrasi Laktosa Dan Sari Kedelai Pada Kualitas Semen Kambing Boer Yang Dipreservasi

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