Kristalline Zelloberflächen-Schichten prokaryotischer Organismen (S-Schichten): von der supramolekularen Zellstruktur zur Biomimetik und Nanotechnologie

Sleytr, Uwe B.; Messner, Paul; Pum, Dietmar; Sára, Margit

doi:10.1002/(sici)1521-3757(19990419)111:8<1098::aid-ange1098>3.0.co;2-f

Cited by 42 publications

(1 citation statement)

References 202 publications

(129 reference statements)

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“…While the reassembly of S-layer proteins at the air-water interface and at planar lipid films is well defined [ 69 , 70 , 78 , 79 , 80 ], the deliberate modification of the surface properties of a solid support allows for specifically controlling the reassembly process [ 71 , 75 , 81 , 82 , 83 ]. For example, the S-layer protein SbpA, which is one of the most extensively studied S-layer proteins for functionalizing solid supports, forms monolayers with a height of 9 nm on the hydrophobic surface.…”

Section: Bacterial S-layer Proteinsmentioning

confidence: 99%

S-Layer Protein-Based Biosensors

Schuster

2018

Biosensors

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The present paper highlights the application of bacterial surface (S-) layer proteins as versatile components for the fabrication of biosensors. One technologically relevant feature of S-layer proteins is their ability to self-assemble on many surfaces and interfaces to form a crystalline two-dimensional (2D) protein lattice. The S-layer lattice on the surface of a biosensor becomes part of the interface architecture linking the bioreceptor to the transducer interface, which may cause signal amplification. The S-layer lattice as ultrathin, highly porous structure with functional groups in a well-defined special distribution and orientation and an overall anti-fouling characteristics can significantly raise the limit in terms of variety and the ease of bioreceptor immobilization, compactness of bioreceptor molecule arrangement, sensitivity, specificity, and detection limit for many types of biosensors. The present paper discusses and summarizes examples for the successful implementation of S-layer lattices on biosensor surfaces in order to give a comprehensive overview on the application potential of these bioinspired S-layer protein-based biosensors.

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Section: Bacterial S-layer Proteinsmentioning

confidence: 99%

S-Layer Protein-Based Biosensors

Schuster

2018

Biosensors

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Nucleic Acid Supercoiling as a Means for Ionic Switching of DNA-Nanoparticle Networks

et al. 2001

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Oligomeric nanoparticle networks, generated by the self-assembly of bis-biotinylated double-stranded DNA fragments and streptavidin, have been studied by scanning force microscopy (SFM). SFM imaging revealed the presence within the networks of irregular thick DNA molecules, which were often associated with distinct, Y-shaped structural elements. Closer analysis revealed that the Y structures are formed by condensation (thickening and shortening) of two DNA fragments, most likely through the supercoiling of two DNA molecules bound to adjacent binding sites of the streptavidin particle. The frequency of supercoiling was found to be dependent on the ionic strength applied during the immobilization of the oligomeric networks on mica surfaces. Potential applications of the structural changes as a means for constructing ion-dependent molecular switches in nanomaterials are discussed.

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