Kristallisation für die Aufarbeitung von Proteinen

Schmidt, Sebastian; Havekost, Dirk; Kaiser, Klaus; Kauling, Jörg; Henzler, Hans‐Jürgen

doi:10.1002/cite.200403360

Cited by 15 publications

(14 citation statements)

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“…Furthermore, crystallized proteins may offer superior properties compared to liquid formulations or amorphous lyophilisates due to better handling, longer shelf-life, and higher purity (Schmidt et al 2004). Furthermore, crystalline pharmaceutical proteins may provide the advantageous possibility of controlled slow release of activity (Basu et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Advanced protein crystallization using water-soluble ionic liquids as crystallization additives

et al. 2007

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The application of five water-soluble, halogen-free, alkylammonium-based ionic liquids (ILs) as additives for advanced crystallization of lysozyme was investigated. Their biocompatibility was determined by long-term measurement of the overall mean relative enzyme activities. These were maximally reduced by about 10-15% when up to 200 g IL l(-1) was added. Sitting-drop vapor diffusion crystallization experiments revealed that the addition of some of the ILs led to less crystal polymorphism and precipitation was avoided reliably even at larger NaCl concentrations. The addition of ILs tended to result in larger crystals. The kinetics of lysozyme crystallization were significantly enhanced using ILs as crystallization additives, e.g. by a factor of 5.5 when 100 g ethanolammonium formate l(-1 )was added. ILs with "soft" anions, such as formate or glycolate, were superior to ILs with "hard" anions, like nitrate.

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Section: Introductionmentioning

confidence: 99%

Advanced protein crystallization using water-soluble ionic liquids as crystallization additives

et al. 2007

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“…Klyushnichenko described the conditions for a large-scale protein crystallization process as a new opportunity for a robust, scaleable, reproducible industrial unit operation by combining high-throughput screening with an engineering approach [11]. In fact, Schmidt et al published such a technical approach using stirred batch crystallizers with two different impeller power inputs of 12 and 640 W m À3 [12]. However, in this work, the protein was not specified, the volumes were not given, and the flow conditions were not described.…”

Section: Introductionmentioning

confidence: 99%

Crystallization of lysozyme: From vapor diffusion experiments to batch crystallization in agitated ml-scale vessels

Hekmat

Hebel

Schmid

et al. 2007

Process Biochemistry

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“…However, chromatography is a laborious and expensive process and therefore it is reasonable to search for alternative purification methods. By crystallization of the proteins, the required purity and yield can be assured [2]. Furthermore, crystallization is a well-established and, compared to chromatography, a low-price process.…”

Section: Introductionmentioning

confidence: 99%

Mechanical Stress on Lysozyme Crystals during Dynamic Cross‐Flow Filtration

2013

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The influence of mechanical stress on the crystal size distribution of lysozyme crystal slurries in a dynamic cross-flow device is discussed. The reduction of crystal size may have an influence on mass and resistance of the fouling layer. A filter test device with a filter area of 130 cm 2 is used for thickening. After thickening, a model impurity, namely bovine serum albumin, is washed out of the suspension. A reduction of crystal size due to stirring and a reduced fouling layer with increasing stirrer speed is observed. Furthermore, a higher stirring speed increases the specific filtrate flux. In the presence of the impurity, more crystal aggregates than single isometric crystals are formed and, hence, the decrease of crystal size due to shear forces is more pronounced than without impurity.

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Kristallisation für die Aufarbeitung von Proteinen

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Cited by 15 publications

References 0 publications

Advanced protein crystallization using water-soluble ionic liquids as crystallization additives

Advanced protein crystallization using water-soluble ionic liquids as crystallization additives

Crystallization of lysozyme: From vapor diffusion experiments to batch crystallization in agitated ml-scale vessels

Mechanical Stress on Lysozyme Crystals during Dynamic Cross‐Flow Filtration

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