Kristallographische Interpretation der Feinstruktur der B-Granula in den Langerhansschen Inseln der Ringelnatter, Natrix n. natrix (L.)

Lange, Rainer; Boseck, S.; Ali, S. Syed

doi:10.1007/bf00306971

Cited by 12 publications

(3 citation statements)

References 18 publications

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“…At the electron microscopic level, Cardeza (19601, using the snake Xenodon merremii, was the first investigator to describe A, B, and D cells in the pancreatic islets of reptiles. Lange et al (1972) carried out a detailed study of the crystalline structure of P-granules in the snake Natrzx natrix.…”

Section: Cells As Well As a And B Cells Were Againmentioning

confidence: 99%

Immunocytochemical localization of four hormones in the pancreas of the garter snake, Thamnophis sirtalis

Rhoten

1984

Anat. Rec.

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Insulin, glucagon, somatostatin, and pancreatic polypeptide (PP) were localized in the pancreas of the common garter snake, Thamnophis sirtalis, by light and transmission electron microscopic (TEM) immunocytochemistry. Colloidal gold-protein A was used for TEM localization and the peroxidase--antiperoxidase complex technique was used for light microscopy. The glucagon-containing A cells and the insulin-positive B cells were the most numerous cell types. The somatostatin-containing D cells made up about 15% of the endocrine cells. PP-positive F cells were a minor cell type. The only topographic arrangement of the cells within the endocrine-rich areas that was apparent was the peripheral localization of the D and F cells. Cells of a specific cell type were sometimes grouped together. At the electron microscopic (EM) level, the gold particles (indicating the presence of hormone) were localized nearly exclusively over the secretory granules of the reactive cells. The alpha-granules were the largest found and were predominantly electron dense with a moderately electron-dense periphery. PP-containing granules were the smallest. The somatostatin-reactive delta-granules were round and moderately electron opaque. The beta-granules were heterogeneous in appearance. The morphognomy of the secretory granules of the major endocrine cell types is qualitatively similar to that of mammals. Whether or not the quantitative and/or associative differences contribute to the marked metabolic differences between reptiles and mammals, remains to be determined.

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Section: Cells As Well As a And B Cells Were Againmentioning

confidence: 99%

Immunocytochemical localization of four hormones in the pancreas of the garter snake, Thamnophis sirtalis

Rhoten

1984

Anat. Rec.

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“…It is worth mentioning that during the maturation of the B granules, their core can be oval or irregular in shape with sharp edges, which is important in the context of its maturity. It can be assumed that the shapes of the core within the B granules in the pancreas of grass snake embryos is typical for the B granules with a crystalline core in different reptilian species, because they correspond with the shapes of the core that have been described within the B granules of the adult grass snake (Trandaburu & Călugareănu, ; Lange, Boseck, & Ali, ), alligator (Raška, Komrska, Titlbach, & Rieder, ), and caiman (Ono et al, ). It is also worth noting that the B granules containing the crystalline‐like core in the embryonic pancreas of the grass snake are similar to the B granules that have been described in the pancreas of most vertebrate species (Bencosme & Pease, ; Pictet & Rutter, ; Hotta, Mabuchi, & Watari, ; Slack, ).…”

Section: Discussionmentioning

confidence: 97%

Ultrastructure of endocrine pancreatic granules during pancreatic differentiation in the grass snake, Natrix natrix L. (Lepidosauria, Serpentes)

Kowalska

Rupik

2017

Journal of Morphology

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We used transmission electron microscopy to study the pancreatic main endocrine cell types in the embryos of the grass snake Natrix natrix L. with focus on the morphology of their secretory granules. The embryonic endocrine part of the pancreas in the grass snake contains four main types of cells (A, B, D, and PP), which is similar to other vertebrates. The B granules contained a moderately electron-dense crystalline-like core that was polygonal in shape and an electron-dense outer zone. The A granules had a spherical electron-dense eccentrically located core and a moderately electron-dense outer zone. The D granules were filled with a moderately electron-dense non-homogeneous content. The PP granules had a spherical electron-dense core with an electron translucent outer zone. Within the main types of granules (A, B, D, PP), different morphological subtypes were recognized that indicated their maturity, which may be related to the different content of these granules during the process of maturation. The sequence of pancreatic endocrine cell differentiation in grass snake embryos differs from that in many vertebrates. In the grass snake embryos, the B and D cells differentiated earlier than A and PP cells. The different sequence of endocrine cell differentiation in snakes and other vertebrates has been related to phylogenetic position and nutrition during early developmental stages.

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“…[7] and [37] for a review). The first attempts to characterize crystalline insulin in secretory granules of the grass snake were made in the early 1970s by Lange et al [38][39][40], based on the three-dimensional reconstruction from serial sections of the snake pancreatic tissue, and by optical diffraction. They found that snake pancreatic granules contain particles in the shape of rhombic dodecahedra.…”

Section: Introductionmentioning

confidence: 99%

Characterization of insulin crystalline form in isolated β-cell secretory granules

et al. 2022

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Insulin is stored in vivo inside the pancreatic β-cell insulin secretory granules. In vitro studies have led to an assumption that high insulin and Zn 2+ concentrations inside the pancreatic β-cell insulin secretory granules should promote insulin crystalline state in the form of Zn 2+ -stabilized hexamers. Electron microscopic images of thin sections of the pancreatic β-cells often show a dense, regular pattern core, suggesting the presence of insulin crystals. However, the structural features of the storage forms of insulin in native preparations of secretory granules are unknown, because of their small size, fragile character and difficult handling. We isolated and investigated the secretory granules from MIN6 cells under near-native conditions, using cryo-electron microscopic (Cryo-EM) techniques. The analysis of these data from multiple intra -granular crystals revealed two different rhomboidal crystal lattices. The minor lattice has unit cell parameters ( a ≃ b ≃ 84.0 Å, c ≃ 35.2 Å), similar to in vitro crystallized human 4Zn 2+ -insulin hexamer, whereas the largely prevalent unit cell has more than double c -axis ( a ≃ b ≃ c ≃ 96.5 Å) that probably corresponds to two or three insulin hexamers in the asymmetric unit. Our experimental data show that insulin can be present in pancreatic MIN 6 cell granules in a microcrystalline form, probably consisting of 4Zn 2+ -hexamers of this hormone.

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Kristallographische Interpretation der Feinstruktur der B-Granula in den Langerhansschen Inseln der Ringelnatter, Natrix n. natrix (L.)

Cited by 12 publications

References 18 publications

Immunocytochemical localization of four hormones in the pancreas of the garter snake, Thamnophis sirtalis

Immunocytochemical localization of four hormones in the pancreas of the garter snake, Thamnophis sirtalis

Ultrastructure of endocrine pancreatic granules during pancreatic differentiation in the grass snake, Natrix natrix L. (Lepidosauria, Serpentes)

Characterization of insulin crystalline form in isolated β-cell secretory granules

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