Kroppenstedtia eburnea gen. nov., sp. nov., a thermoactinomycete isolated by environmental screening, and emended description of the family Thermoactinomycetaceae Matsuo et al. 2006 emend. Yassin et al. 2009

Abstract

A Gram-positive, spore-forming, aerobic, filamentous bacterium, strain JFMB-ATET, was isolated in 2008 during environmental screening of a plastic surface in grade C in a contract manufacturing organization in southern Germany. The isolate grew at temperatures of 25–50 °C and at pH 5.0–8.5, forming ivory-coloured colonies with sparse white aerial mycelia. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the family Thermoactinomycetaceae, except that the cell-wall peptidoglycan contained ll-diaminopimelic acid, while all previously described members of this family display this diagnostic diamino acid in meso-conformation. The DNA G+C content of the novel strain was 54.6 mol%, the main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, and the major menaquinone was MK-7. The major fatty acids had saturated C14–C16 branched chains. No diagnostic sugars were detected. Based on the chemotaxonomic results and 16S rRNA gene sequence analysis, the isolate is proposed to represent a novel genus and species, Kroppenstedtia eburnea gen. nov. sp. nov. The type strain is JFMB-ATET ( = DSM 45196T = NRRL B-24804T = CCUG 59226T).

Kroppenstedtia pulmonis sp. nov. and Kroppenstedtia sanguinis sp. nov., isolated from human patients

“…The genomic DNA G+C content of strains W9323 T and X0209 T was 45.9 and 50.6 mol%, respectively. These values fall within the range of genomic DNA G+C content reported for the genus Kroppenstedtia as it exists presently (46 to 56 mol%) (von Jan et al 2011, Yang et al 2013.…”

Isolation and Identification of Kroppenstedtia eburnea Isolates from Multiple Patient Samples

“…Members of the Thermoactinomycetaceae are rarely isolated from patient specimens and are difficult to identify by conventional phenotypic testing. Their recognition has been facilitated by molecular identification methods, and several novel species have recently been described (1,2,5,12,14,15). Here we showed that K. eburnea has been repeatedly isolated from clinical specimens in different geographic regions of the United States.…”

A Systematic Approach for Discovering Novel, Clinically Relevant Bacteria

“…These species were not identified in the automated analysis due to short reference sequences or because they had a subspecies annotation not covered in the algorithm. The validity of our approach was confirmed, however, when a novel thermoactinomycete, Kroppenstedtia eburnea ( 32 ), was formally described during preparation of this article. The 16S sequence of this organism showed ≈99.5% identity to a large cluster of 12 isolates in our study (Table 2).…”

“…thermoviolaceus . One cluster of 12 isolates belonged to a novel genus and species, Kroppenstedtia eburnea , which was described subsequent to our initial analysis ( 32 ). …”