Krüppel-like factor 14, a coronary artery disease associated transcription factor, inhibits endothelial inflammation via NF-κB signaling pathway

Hu, Wenting; Lu, Haocheng; Zhang, Jifeng; Fan, Yanbo; Chang, Ziyi; Liang, Wenying; Wang, Huilun; Zhu, Tianqing; Garcia-Barrio, Minerva T.; Peng, Daoquan; Chen, Y. Eugene; Guo, Yanhong

doi:10.1016/j.atherosclerosis.2018.09.018

Cited by 34 publications

(29 citation statements)

References 41 publications

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“…endothelial cell structural and functional damage occurs at the beginning of cHd (5). endothelial cells are the inner layers of vascular walls and are important regulators of vascular inflammation, blood aggregation, vascular tension and capillary permeability (45). resting endothelial cells express no or low levels of adhesion molecules and are resistant to leukocyte recruitment.…”

Section: Discussionmentioning

confidence: 99%

Protective effect of microRNA‑381 against inflammatory damage of endothelial cells during coronary heart disease by targeting CXCR4

Huang

Hong

et al. 2020

Mol Med Report

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coronary heart disease (cHd) is the leading cause of human morbidity and mortality worldwide. Microrna (mirna) profiling is an innovative method of identifying biomarkers for many diseases and may be a powerful tool in the diagnosis and treatment of cHd. The present study aimed to analyze the effects of miRNA (miR)-381 on the inflammatory damage of endothelial cells during cHd. a total of 21 patients with cHd and 21 healthy control patients were enrolled in this study. reverse transcription-quantitative PCR, western blotting and immunofluorescence assays were conducted to examine the expression levels of mir-381, c-X-c chemokine receptor type 4 (cXcr4), Bcl-2, Bax, cleaved-caspases-3 and-9, p38, erK1/2 and JnK. cell Counting Kit-8, EdU and flow cytometry experiments were performed to evaluate cell proliferation and apoptosis. an ELISA was adopted to determine the expressions of inflammatory factors (interleukins-8,-6 and-1β, and tumor necrosis factor-α). in addition, a dual-luciferase reporter assay was used to determine the relationship between mir-381 and cXcr4. decreased mir-381 expression and increased cXcr4 expression in the plasma were observed in the cHd group compared with the normal group, which indicated a negative relationship between mir-381 and cXcr4. overexpression of mir-381 significantly promoted the proliferation and inhibited the apoptosis of oxidized low-density lipoprotein (oX-ldl)-induced human umbilical vein endothelial cells (HuVecs) through mitogen-activated protein kinase pathway by targeting and inhibiting cXcr4. Furthermore, overexpression of miR-381 reduced the release of inflammatory factors in oX-ldl-induced HuVecs. By contrast, reduced expression of mir-381 exerted the opposite effects, which were subsequently reversed by silencing cXcr4 expression. results from the present study indicated that mir-381 was a cHd-related factor that may serve as a potential molecular target for cHd treatment.

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Section: Discussionmentioning

confidence: 99%

Protective effect of microRNA‑381 against inflammatory damage of endothelial cells during coronary heart disease by targeting CXCR4

Huang

Hong

et al. 2020

Mol Med Report

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“…KLF14 is a zinc finger DNA-binding protein belonging to the Specificity protein/Kruppel-like factor (SP/KLF) family [70]. Both the KLF14 mRNA and protein levels were reduced in lung tissues from lipopolysaccharide-treated mice and KLF14 overexpression inhibited inflammation in human coronary artery endothelial cells (HCAECs) and human umbilical vein endothelial cells (HUVECs) [71]. Taken together, it appears that KLF14-derived peptide may contribute to inflammation inhibition in neonatal respiratory diseases.…”

Section: Discussionmentioning

confidence: 99%

Peptidome Analysis of Umbilical Cord Mesenchymal Stem Cell (hUC-MSC) Conditioned Medium from Preterm and Term Infants

Wang

Zhang

et al. 2020

Preprint

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Background : The therapeutic role of mesenchymal stem cells (MSCs) has been widely confirmed in several animal models of premature infant diseases. Micromolecule peptides have shown promise for the treatment of premature infant diseases. However, the potential role of peptides secreted from MSCs has not been studied. The purpose of this study is to broaden the knowledge of the hUC-MSC secretome at the peptide level through peptidomic profile analysis. Methods : We used tandem mass tag (TMT) labeling technology followed by tandem mass spectrometry to compare the peptidomic profile of preterm and term umbilical cord MSC (hUC-MSC) conditioned medium (CM). Gene Ontology (GO) enrichment analysis and Ingenuity Pathway Analysis (IPA) were conducted to explore the differentially expressed peptides by predicting the functions of their precursor proteins. And we investigated the effects of differentially expressed peptides on human lung epithelial cells stimulated by hydrogen peroxide (H 2 O 2 ). Results : A total of 131 peptides derived from 106 precursor proteins were differentially expressed in the preterm hUC-MSC CM compared with the term group, comprising 37 up-regulated peptides and 94 down-regulated peptides. Bioinformatics analysis showed that these differentially expressed peptides may be associated with developmental disorders, inflammatory response and organismal injury. We also found that two differentially expressed peptides reduced the levels of TNFα and IL 1β mRNA in H 2 O 2 -treated human lung epithelial cells. Conclusions : In summary, this study provides further secretomics information on hUC-MSCs and provides a series of peptides that might have roles in premature infant diseases, which may be helpful for prevention and treatment.

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“…KLF14 may be a protective factor against atherosclerosis . In addition, KLF14 hampered atherosclerosis development via inhibiting inflammation in endothelium, indicating endothelial KLF14 has potential to serve as a target for the treatment of atherosclerotic diseases …”

Section: Klf14 and Cholesterol Metabolismmentioning

confidence: 99%

The role of KLF14 in multiple disease processes

2020

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Kruppel‐like factor 14 (KLF14) is a newly identified member of the KLF family. Expression of KLF14 is induced by TGF‐β in intrauterine and ectodermal tissue. Initial researches on KLF14 focused on its role in lipid and glucose metabolism. In recent years, however, the role of KLF14 in regulating cell signaling pathways, cell proliferation and differentiation has been explored. Moreover, the research has gradually extended into the field of tumorigenesis and immune regulation. This paper aims to briefly review the functions of KLF14 in physiologyical and pathological process.

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Krüppel-like factor 14, a coronary artery disease associated transcription factor, inhibits endothelial inflammation via NF-κB signaling pathway

Abstract: The data revealed that KLF14 inhibited the inflammatory response in ECs and the protective effects were mediated by transcriptional inhibition of NF-κB signaling pathway. Endothelial KLF14 could be a potential therapeutic target for cardiovascular diseases.

Cited by 34 publications

References 41 publications

Protective effect of microRNA‑381 against inflammatory damage of endothelial cells during coronary heart disease by targeting CXCR4

Protective effect of microRNA‑381 against inflammatory damage of endothelial cells during coronary heart disease by targeting CXCR4

Peptidome Analysis of Umbilical Cord Mesenchymal Stem Cell (hUC-MSC) Conditioned Medium from Preterm and Term Infants

The role of KLF14 in multiple disease processes

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