Krüppel-like factors in glycolipid metabolic diseases

Li, Yutong; Zhao, Xiaotong; Xu, Murong; Chen, Mingwei

doi:10.1007/s11033-022-07565-0

Cited by 7 publications

(1 citation statement)

References 59 publications

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“…It is known that KLFs are a family of transcription factors characterized by zinc-finger structures at the C-terminal end, and they play critical roles in cell proliferation, differentiation, and migration [49]. Using BLAST and HMM, we identified 56 KLF genes from four species and then a total of 13 KLF genes with specific conserved-domain zf-H2C2 genes were identified in G. gallus.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of the KLF Gene Family in Chicken: Characterization and Expression Profile

Ling

Wang

Zhang

et al. 2023

Animals

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The kruppel-like factor (KLF) gene family is a group of transcription factors containing highly conserved zinc-finger motifs, which play a crucial role in cell proliferation and differentiation. Chicken has been widely used as a model animal for analyzing gene function, however, little is known about the function of the KLF gene family in chickens. In this study, we performed genome-wide studies of chicken KLF genes and analyzed their biological and expression characteristics. We identified 13 KLF genes from chickens. Our phylogenetic, motif, and conserved domain analyses indicate that the KLF gene family has remained conserved through evolution. Synteny analysis showed the collinear relationship among KLFs, which indicated that they had related biomolecular functions. Interaction network analysis revealed that KLFs worked with 20 genes in biological processes. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that KLF2 was involved in Apelin and Forkhead Box O (FOXO) signaling pathways. Moreover, qPCR showed that 13 KLF genes were expressed in the nine selected tissues and displayed various gene expression patterns in chickens. RNA-seq showed that KLF3 and KLF10 genes were differentially expressed in the normal and high-fat diet fed groups, and KLF4, KLF5, KLF6, KLF7, KLF9, KLF12, and KLF13 genes were differentially expressed between undifferentiated and differentiated chicken preadipocytes. Besides, RNA-seq also showed that KLF genes displayed different expression patterns in muscle at 11 and 16 embryonic days old, and in 1-day-old chickens. These results indicated that the KLF genes were involved in the development of muscle and fat in chickens. Our findings provide some valuable reference points for the subsequent study of the function of KLF genes.

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Section: Discussionmentioning

confidence: 99%