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Abstract

Artificial reproduction of common barbel (Barbus barbus) under controlled conditions usually did not exceed 10% of ovulation success. The aim of the study was to optimise the process of artificial reproduction of the barbel (cultured generation F4) with thermal and hormonal stimulation The first experiment examined the effect of stimulation with thermal conditions on the ovulation and the embryos survival rates. After the optimum conditions of thermal stimulation in barbel reproduction were determined, another experiment was conducted which examined how the effectiveness of reproduction is affected by four selected hormonal preparations (CPH, hCG, Ovaprim and Ovopel). Thermal stimulation for 58 days was found to be the most effective (748 degree-days). The second experiment examined the synergistic effects of different hormonal preparations under the optimal thermal conditions as, determined in the first experiment. Among the hormones evaluated as part of this study, CPH and Ovaprim were yielded the best results as compared to control. The use of the preparations resulted in the percentage of ovulations of 90-100% and the embryo survival rate at the hatching stage was about 90%. The study concludes that thermal stimuli and hormonal applications have a synergistic effect on artificial reproduction in B, barbus.

Artificial Reproduction of Blue Bream (Ballerus ballerus L.) as a Conservative Method under Controlled Conditions

“…The embryo survival rate in this group was only slightly lower than the rate for embryos hatched from the eggs from Ovopel-injected females. A similar observation was made for common dace L. leuciscus [11,20], asp L. aspius [12], ruffe Gymnocephalus cernua [31], common barbel Barbus barbus [32], river lamprey Lampetra fluviatilis [34], European catfish S. glanis [46], ide L. idus [48,54] and burbot L. lota [55]. The present study clearly shows that Ovopel is the best reproduction stimulator for blue bream caught in the wild.…”

“…Thus, the possibility of its excretion by some females and the impact on other breeders in our experiment was imperceptible, as evidenced by the results from the control group (ovulation-0%). Determination of the optimal stimulation conditions, including simultaneous environmental conditions and hormonal agents, is necessary for the effective artificial reproduction of cyprinids [32]. The studies conducted thus far on cyprinids have shown that off-season breeding, although more difficult to carry out, requires the use of thermal conditions that are the same as those used in the season [11,13,14,32].…”

“…Determination of the optimal stimulation conditions, including simultaneous environmental conditions and hormonal agents, is necessary for the effective artificial reproduction of cyprinids [32]. The studies conducted thus far on cyprinids have shown that off-season breeding, although more difficult to carry out, requires the use of thermal conditions that are the same as those used in the season [11,13,14,32]. The results of the reproduction of fish of the genus Abramis, which are related to the genus Ballerus, reported thus far have shown that the reproduction of fish in the season and in the off-season is very similar in terms of the reproductive protocol [62][63][64][65][66][67].…”

“…The most common agents administered to fish include carp pituitary homogenate (CPH), human chorionic gonadotropin (hCG), Ovopel containing a mammalian gonadotropin-releasing hormone (GnRH) analogue and metoclopramide as a dopamine receptor antagonist and Ovaprim containing an analogue of salmon GnRH and domperidone as a dopamine receptor antagonist [12,17,30,31]. Most hormonal agents require proper preparationthey must be dissolved or homogenized in a 0.9% NaCl solution [32][33][34]. Among the hormonal agents mentioned above, only Ovaprim is immediately ready for use.…”

Degree-hours to spawning response, fertilization rate and hatching of Labeo rohita and Cirrhinus mrigala through induced breeding under hatchery environment.

“…In every trail each species was divided into four groups. Outcomes were calculated after treatments of ovaprim hormone (Nowosad et al, 2016;Naeem et al, 2005). These results show that degree-hours directly affect the spawning response and…”