2007
DOI: 10.1016/j.molcel.2007.05.025
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Ku Suppresses Formation of Telomeric Circles and Alternative Telomere Lengthening in Arabidopsis

Abstract: Telomeres in mammals and plants are protected by the terminal t loop structure, the formation of which parallels the first steps of intrachromatid homologous recombination (HR). Under some circumstances, cells can also utilize an HR-based mechanism (alternative lengthening of telomeres [ALT]) as a back-up pathway for telomere maintenance. We have found that the Ku70/80 heterodimer, a central nonhomologous end-joining DNA repair factor, inhibits engagement of ALT in Arabidopsis telomerase-negative cells. To fur… Show more

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Cited by 112 publications
(160 citation statements)
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“…That Ku might function through a distinct pathway is also supported by findings in Arabidopsis, in which Ku was COMMENTARY first implicated in the suppression of t-circle formation (14). T-circles arise in Ku-deficient plants; however, neither Mre11 nor any of the Rad51 paralogs, including XRCC3, are required for their formation.…”
mentioning
confidence: 74%
“…That Ku might function through a distinct pathway is also supported by findings in Arabidopsis, in which Ku was COMMENTARY first implicated in the suppression of t-circle formation (14). T-circles arise in Ku-deficient plants; however, neither Mre11 nor any of the Rad51 paralogs, including XRCC3, are required for their formation.…”
mentioning
confidence: 74%
“…In any case, there is precedent for Ku regulating telomere loss. In plants, deletion of Ku also results in the formation of t-circles (31). However, the parallel to plants is limited because Ku-deficient plants are viable and aphenotypic.…”
Section: Discussionmentioning
confidence: 99%
“…Neutral-neutral 2D gel electrophoresis was performed as described previously (26,31) with the following modifications: precipitated DNA was digested with AluI in the presence of 30 g/mL RNaseA. This AluI-digested DNA (8 g) was separated in 0.4% agarose gels in 1ϫ TBE buffer at 1 V/cm for 18 h at room temperature.…”
Section: Methodsmentioning
confidence: 99%
“…Telomere length measurement is now widely used and our experience here is that the heterozygotes, who are often more elderly, may have telomere lengths that are short, but not necessarily very short. We have also looked at T-circles 15 and shown that, in some cases, their presence is clearly increased where there is a loss of function variant compared to a common missense variant ( Figure 1E). However, this test is not easy to perform, and a normal range has not been established.…”
Section: Letters To the Editormentioning
confidence: 97%