Kukoamine A analogs with lipoxygenase inhibitory activity

α-Amination and the 5-exo-trig cyclization reaction of sulfur-containing Schiff bases with N-phenyltriazolinedione and their anti-lipid peroxidation activity

“…[36]) Production of conjugated diene hydroperoxide by oxidation of linoleic acid in an aqueous dispersion was monitored at 234 nm. 2, 2 0 -Azobis(2-amidinopropane) dihydrochloride (AAPH) is used as a free radical initiator.…”

“…LTB4 generation is considered to be important in the pathogenesis of neutrophil-mediated inflammatory diseases [35] with a marked relation to the severity of cardiovascular diseases, asthma and cancer. In this context, we decided to further evaluate the synthesized cycloadducts as soybean LOX inhibitors by the UV absorbance based enzyme assay [36]. NDGA, a known inhibitor of soybean LOX, has been used as a reference compound (83% or 45 mМ) and as a positive control.…”

“…[36]) The in vitro study was evaluated as reported previously. The test compounds dissolved in ethanol were incubated at room temperature with sodium linoleate (100 mM) and 0.2 mL of enzyme solution (1/9 Â 10 À4 w/v in saline).…”

Styryl and functionalized aryl derivatives of lawsone through metal-free cross-coupling of its BF3-activated phenyliodonium ylide with cinnamaldehydes and arylaldehydes

“…Soybean Lipoxygenase, linoleic acid sodium salt, trolox and 2, 2 0 -Azobis(2-amidinopropane) dihydrochloride were obtained from Sigma Chemical, Co. 51,52 The compounds were dissolved in ethanol and incubated at room temperature with sodium linoleate (100 mM) and 0.2 mL of enzyme solution (1/9Â10 À4 w/v in saline). The conversion of sodium linoleate to 13-hydroperoxylinoleic acid at 234 nm was recorded and compared with the appropriate standard inhibitor (nordihydroguaeretic acid) ( 51,52 Production of conjugated diene hydroperoxide by oxidation of linoleic acid in an aqueous dispersion was monitored at 234 nm. 2, 2 0 -Azobis(2-amidinopropane) dihydrochloride (AAPH) is used as a free radical initiator.…”

Preventing H2O2-induced toxicity in primary cerebellar granule neurons via activating the PI3-K/Akt/GSK3β pathway by kukoamine from Lycii Cortex

“…More than 20 constituents have been phytochemically identified from LyC, which include spermine alkaloids (Funayama, Zhang, & Nozoe, 1995), peptides (Yahara et al, 1993), phenolic acids, coumarins and other compounds (Potterat, 2010;Yao et al, 2011). Spermine alkalosis, mainly kukoamines A and B, were reported as the major and characteristic compounds in LyC , and possess obvious anti-oxidative (Hadjipavlou-Litina, Garnelis, Athanassopoulos, & Papaioannou, 2009) and antiinflammatory properties . The cyclicoctapeptides, the second major components represented by lyciumins A, B, C and D (Zhang et al, 2013), were effective in antagonizing the Angiotensin-converting enzyme (Yahara, Shigeyama, & Nohara, 1989).…”