Kultur Kalus Tanaman Obat Physalis angulata L. (Ciplukan)

Mastuti, Retno; Widoretno, Wahyu; Harijati, Nunung

doi:10.21776/ub.biotropika.2020.008.01.05

Cited by 4 publications

(6 citation statements)

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“…Meanwhile, the petiole explants occurred at 42 DAI. According to Mastuti et al [11], the formation of a callus is obtained in two stages, namely induction and proliferation. During the tissue induction stage, the explants undergo a dedifferentiation process that causes the cells that make up the mature leaf tissue to turn into meristematic cells.…”

Section: Resultsmentioning

confidence: 99%

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Morphogenesis Response of Leaf and Petiole Explant of Rootstock Apple (Malus sylvestris Mill.) to Auxin and Cytokinin

Nafika,

Munawarti

2024

Jurnal Biotropika

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Rootstock apple is a type of plant used in grafting propagation. Research related to the response of morphogenesis in this plant has not been widely carried out. The aim of this research was to study the effect of auxin and cytokinin ratio on the morphogenesis response of leaf and petioles explants of rootstock apple and to study variations in the ratio of auxin and cytokinin with the best response. The study used a 2-factorial Completely Randomized Design (CRD) with variations in the types of explants and combinations of plants growth regulators (PGR) in the culture medium. There were 12 treatments with five repetitions. Explants in the form of young apple leaf (third leaf) and petiole rootstock that had been sterilized and cut with a size of 5x5 mm for the leaf and 5 mm long for the petiole were inoculated into MS medium with cytokinin in the form of BAP (1 ppm) combined with auxin, IBA (0, 0.1, 0.2, 0.3, 0.4, 0.5 ppm). Each culture bottle contains five explants. The results showed that the average leaf explant was capable of producing 34% callus. Meanwhile, petiole explants produced 4% callus. Callus began to form on leaf and petiole explants on the 26th and 42nd days after inoculation respectively. Most calluses have a compact texture with variations in green, yellowish white, and brownish green. The PGR combination with the highest percentage of callus formation in leaf explants was found in the BAP 1 ppm + IBA 0.4 ppm treatment, while the petiole was in the BAP 1 ppm + IBA 0.3 ppm treatment

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Section: Resultsmentioning

confidence: 99%

“…This shows that the cells in the explants are still actively dividing to form a callus. According to Mastuti et al [11], cleavage continues continuously until the formation of callus masses can occur due to the availability of appropriate exogenous and endogenous hormones.…”

Section: Figure 2 Percentage Of Petiole Explants Forming Calluses At ...mentioning

confidence: 99%

Morphogenesis Response of Leaf and Petiole Explant of Rootstock Apple (Malus sylvestris Mill.) to Auxin and Cytokinin

Nafika,

Munawarti

2024

Jurnal Biotropika

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“…Hendaryono & Wijayani, (1994) sumber eksplan yang baik adalah bagian tanaman yang mempunyai banyak jaringan pengangkut. Mastuti et al, (2020) memperoleh bahwa pemberian 1,2 mg/L dan 4 mg/l 2,4 D yang dikombinasikan dengan 2 mg/l BAP pada media Murashoge Skoog (MS) mampu meningkatkan bobot basah kalus dari hipokotil ciplukan (Physalis angulata L.). Penelitian Zulkarnain, & Lizawati (2011) melaporkan bahwa pemberian 1-5 mg/L 2,4-D pada kultur hipokotil tanaman jarak pagar (Jatropha curcas L.) dapat memicu pembentukan dan perbanyakan kalus.…”

Section: Pendahuluanunclassified

Induksi Kalus dari Hipokotil Belimbing Merah (Baccaurea angulata) dengan Penambahan 2,4-D (Dichlorophenoxy Acetic Acid) dan BAP (6-Benzyl Amino Purin)

Teresia,

Zakiah,

Turnip

2024

JBT

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Red starfruit is one of the endemic plants of Kalimantan which produces secondary metabolites which has been empirically used as antioxidant, antibacterial, and anticancer. This plant has a long reproductive period and seedless fruit is often found during the fruiting season, which is a problem for the sustainability of the red starfruit in nature. Conservation of red star fruit for propagation and production of secondary metabolites can be done in vitro through callus culture. This research aims to determine the effect of adding the growth regulators dichlorophenoxy acetic acid (2,4-D) and 6-benzyl amino purine (BAP) on callus induction time, percentage of callus forming explants, callus color and texture, and callus growth. This research used a Completely Randomized Design (CRD) with a factorial pattern consisting of two treatment factors, namely 2,4-D (0; 0.25; 0.5; 0.75; 1 ppm) and BAP (0; 0.25; 0.5; 0.75; 1 ppm). The percentage of explants forming callus (%), the time the callus appeared (days after planting), the color and texture of the callus, the wet weight and dry weight of the callus (g) were research observation parameters.The results showed that the single factor 2,4-D, single factor BAP, and the interaction of 2,4-D and BAP had a significant effect to callus appearance time. Single administration of 2,4-D and BAP had a significant effect on callus dry weight. The color variations of the callus produced are white, green and brown with a compact texture. The fastest callus emergence time was at 11.00 days after planting (DAP), namely in the combination treatment of 1 ppm 2,4-D and 0.75 ppm BAP, single treatment 0.75 ppm and 1 ppm 2,4- D. The highest average dry weight of 0.024 g was obtained in the single treatment of 0.75 ppm BAP.

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“…Berdasarkan hasil uji lanjut tersebut didapatkan bahwa perlakuan N2B2 berbeda nyata dengan perlakuan lainnya. Mastuti et. al.…”

Section: Berat Kalusunclassified

INISIASI KALUS SECARA IN VITRO DARI DAUN Talinum paniculatum (Jacq.) Gaertn.

Afiyah

Surya²,

Ismaini³

et al. 2022

BKR

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Ginseng Jawa (Talinum paniculatum (Jacq.) Gaertn.) merupakan terna yang daunnya memiliki kandungan metabolit sekunder dan dapat digunakan sebagai obat. Beberapa penelitian kultur jaringan terkait sterilisasi dan penambahan zat pengatur tumbuh pada ginseng Jawa masih belum banyak dilakukan. Tujuan penelitian ini adalah mengetahui metode sterilisasi yang tepat serta melakukan optimalisasi zat pengatur tumbuh Naphthalene Acetic Acid (NAA) dan Benzylaminopurine (BAP) untuk menginisiasi kalus ginseng Jawa. Penelitian ini dilaksanakan di Laboratorium Kultur Jaringan Kebun Raya Cibodas – BRIN pada bulan Agustus sampai November 2021. Metode penelitian yang digunakan adalah metode eksperimental menggunakan Rancangan Acak Lengkap (RAL) non faktorial dengan sembilan kombinasi perlakuan penambahan NAA dan BAP yang diulang sebanyak tiga kali. Eksplan daun berdiameter 7 mm ditanam pada botol kultur berisi media Murashige and Skoog yang mengandung kombinasi konsentrasi zat pengatur tumbuh NAA (0,5; 1; dan 1,5 ppm) dan BAP (0,5; 1; dan 1,5 ppm). Hasil penelitian menunjukkan bahwa eksplan yang ditanaman mampu menghasilkan kalus. Waktu kemunculan kalus antara 11-14 hari setelah inisiasi dan memiliki tekstur kompak pada semua perlakuan. Selain itu, empat warna kalus yang dihasilkan dari perlakuan ini, yaitu light olive brown, olive gray, gray brown, dan olive. Kombinasi konsentrasi terbaik diperoleh pada perlakuan N2B2 (1 ppm NAA + 1 ppm BAP). Perlakuan tersebut memberikan pertumbuhan optimal pada parameter panjang kalus sebesar 3,13 cm dan berat basah kalus sebesar 2,27 g.

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Kultur Kalus Tanaman Obat Physalis angulata L. (Ciplukan)

Cited by 4 publications

References 0 publications

Morphogenesis Response of Leaf and Petiole Explant of Rootstock Apple (Malus sylvestris Mill.) to Auxin and Cytokinin

Morphogenesis Response of Leaf and Petiole Explant of Rootstock Apple (Malus sylvestris Mill.) to Auxin and Cytokinin

Induksi Kalus dari Hipokotil Belimbing Merah (Baccaurea angulata) dengan Penambahan 2,4-D (Dichlorophenoxy Acetic Acid) dan BAP (6-Benzyl Amino Purin)

INISIASI KALUS SECARA IN VITRO DARI DAUN Talinum paniculatum (Jacq.) Gaertn.

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