Kurthia huakuii sp. nov., isolated from biogas slurry, and emended description of the genus Kurthia

Antonie van Leeuwenhoek

et al. 2015

A novel facultatively anaerobic bacterial strain, designated LAM0504(T), was isolated from a pit mud of Luzhou flavour liquor alcohol fermentation in Sichuan Province, China. Cells of strain LAM0504(T) were observed to be Gram-stain negative, spore-forming, rod shaped and motile by means of peritrichous flagella. Strain LAM0504(T) was found to be able to grow at 20-48 °C (optimum: 30 °C), pH 5.0-9.0 (optimum: 7.0) and 0-3 % NaCl (w/v) (optimum: 1.0 %). The 16S rRNA gene sequence similarity analysis showed that strain LAM0504(T) was most closely related to Paenibacillus konsisdensis JCM 14798(T), Fontibacillus phaseoli LMG 27589(T) and Paenibacillus motobuensis JCM 12774(T), with 97.0, 96.8 and 96.7 % sequence similarity, respectively. The DNA-DNA hybridization value between strain LAM0504(T) and P. konsisdensis JCM 14798(T) was 53.3 ± 1.2 %. The genomic DNA G+C content of strain LAM0504(T) was 43.0 mol% as determined by the Tm method. The major fatty acids of strain LAM0504(T) were identified as anteiso-C15:0, C16:0 and iso-C15:0. The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid. The predominant menaquinone was identified as MK-7. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, two unidentified glycolipids and three unidentified lipids. On the basis of its physiological and phylogenetic characteristics, strain LAM0504(T) is concluded to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus vini sp. nov. is proposed. The type strain is LAM0504(T) (=ACCC 06420(T) = JCM 19842(T)).

Section: Morphological Physiological and Biochemical Characteristicsmentioning

confidence: 99%

Paenibacillus vini sp. nov., isolated from alcohol fermentation pit mud in Sichuan Province, China

Chen

Shao

Antonie van Leeuwenhoek

et al. 2015

“…Growth at different temperatures (10,15,25,30,35,45,50,55 and 60 u C), pH (3, 4, 4.5, 5, 6, 7, 8, 9, 10, 10.5, 11 and 12) and NaCl concentrations (0.5, 1, 2, 3, 4, 5, 6, 7, 8, 10, 11 and 12 %) were determined in TSB medium (BD/ Difco211825) over 3 days. The medium pH was adjusted as described by Ruan et al (2014). All tests were conducted independently in duplicate.…”

Section: Strain Lam612mentioning

confidence: 99%

Lysinibacillus halotolerans sp. nov., isolated from saline-alkaline soil

Kong

International Journal of Systematic and Evolutionary Microbiology

Zhao

et al. 2014

Self Cite

A novel aerobic, halotolerant bacterium, designated strain LAM612T , was isolated from salinealkaline soil samples from Lingxian County, Shandong Province, China. Cells of strain LAM612T were Gram-reaction-positive, endospore-forming, motile and rod-shaped. The optimal temperature and pH for growth were 35 6C and pH 6.0, respectively. Strain LAM612 T could grow in the presence of up to 10 % (w/v) NaCl. The genomic DNA G+C conten was 36. ranged from 20.6 % to 41.9 %. The major fatty acids of strain LAM612 T were iso-C 15 : 0 (40.8 %), iso-C 16 : 0 (15.2 %) and anteiso-C 15 : 0 (10.8 %). The cell-wall peptidoglycan content was A4a (LLys-D-Asp). The predominant menaquinone was MK-7 and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unknown phospholipids, five unknown glycolipids and an unknown lipid. Based on the DNA-DNA hybridization results and phenotypic, phylogenetic and chemotaxonomic properties, strain LAM612 T could be distinguished from the recognized species of the genus Lysinibacillus, and was suggested to represent a novel species of this genus, for which the name Lysinibacillus halotolerans sp. nov. is proposed. The type strain is LAM612 T (5ACCC 00718 T 5JCM 19611 T ).

“…NaCl concentrations of 0, 1, 2, 3,4,5,6,7,8,9,10,11 and 12 % (w/v) were produced in TSB medium prepared according to the formula of TSB but varying the addition of NaCl. The medium was adjusted to the desired pH by using sterile solutions of citric acid/ Na 2 HPO 4 (pH 4.0-5.0), MES (pH 5.5-6.0), PIPES (pH 6.5-7.0), Tricine (pH 7.5-8.5) or CAPSO (pH 9.0-9.5) added at a final concentration of 30 mM (Ruan et al 2014). All tests were conducted independently in duplicate.…”

Section: Methodsmentioning

confidence: 99%

Paenibacillus populi sp. nov., a novel bacterium isolated from the rhizosphere of Populus alba

Han

Tong

Antonie van Leeuwenhoek

et al. 2015

A novel aerobic bacterium, designated strain LAM0705(T), was isolated from the rhizosphere of Populus alba in the Peking University Third Hospital. Cells of strain LAM0705(T) were observed to be Gram-stain positive, motile, spore-forming and rod-shaped. The optimal temperature and pH for growth were found to be 30 °C and pH 7.5, respectively. Strain LAM0705(T) was found to be able to grow in the presence 0-5 % NaCl (w/v) (optimum 1.0 %). The major fatty acids of strain LAM0705(T) were identified as anteiso-C15:0, C16:0 and iso-C16:0. The dominant polar lipids were found to consist of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The cell wall peptidoglycan of strain LAM0705(T) was found to contain meso-diaminopimelic acid. The predominant menaquinone was identified as MK-7. The G+C content of genomic DNA was found to be 48 mol% when determined by the T m method. The 16S rRNA gene sequence similarity analysis indicated that strain LAM0705(T) is closely related to Paenibacillus agaridevorans DSM 1355(T) and Paenibacillus thailandensis KCTC 13043(T) with 97.8 and 96.1 % sequence similarity, respectively. The DNA-DNA hybridization value between strain LAM0705(T) and P. agaridevorans DSM 1355(T) was 47 ± 0.8 %. On the basis of its phenotypic, phylogenetic and chemotaxonomic characteristics, strain LAM0705(T) is concluded to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus populi sp. nov. is proposed. The type strain is LAM0705(T) (=ACCC 06427(T) = JCM 19843(T)).