Kyrpidia spormannii sp. nov., a thermophilic, hydrogen-oxidizing, facultative autotroph, isolated from hydrothermal systems at São Miguel Island, and emended description of the genus Kyrpidia

Reiner, Johannes Eberhard; Jung, Tobias; Lapp, Christian Jonas; Siedler, Marvin; Bunk, Boyke; Overmann, Jörg; Gescher, Johannes

doi:10.1099/ijsem.0.003037

Cited by 33 publications

(22 citation statements)

References 26 publications

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“…was observed on the following sugars: glucose, galactose, fructose, maltose, ribose and lactose. This is in contrast to K. spormannii EA-1, since this strain could utilize pyruvate and sugars (Reiner et al, 2018). However, K. tusciae did not show growth on sugars and pyruvate either (Bonjour and Aragno, 1984).…”

Section: Growth On Other Substratesmentioning

confidence: 60%

“…K. spormannii EA-1 does not grow using urea or N 2 as nitrogen source. The genome of this strain does not contain a urease gene, but the nif -genes could be identified (Reiner et al, 2018). The genome of K. tusciae contains an urease and growth on urea has been reported, but no N 2 fixation has been observed (Bonjour and Aragno, 1984;Klenk et al, 2011).…”

Section: Growth On Other Substratesmentioning

confidence: 85%

“…The genus Kyrpidia, with thus far two cultured representatives, is the second genus in the parent family Alicyclobacillaceae (Klenk et al, 2011). Kyrpidia species, including our isolates, are Gram-stain-positive, aerobic, endospore-forming, non-motile rods (0.7-1.2 × 4-8 µm) (Reiner et al, 2018). To distinguish between species level, the average nucleotide identity (ANI) was calculated (Goris et al, 2007).…”

Section: Genome Sequencing and Phylogenymentioning

confidence: 99%

“…So far, two other Kyrpidia species have been isolated from geothermal areas. K. spormannii EA-1 was isolated from geothermal soils on the Azores, São Miguel, Portugal (Reiner et al, 2018) and K. tusciae was isolated from a geothermal pond in Tuscany, Italy (Bonjour and Aragno, 1984). 16S rRNA gene sequencing of environmental samples showed that members of the genus Kyrpidia were also found in sugarcane bagasse feedstock piles which are slightly acidic with a temperature of 49-52 • C (Rattanachomsri et al, 2011).…”

Section: Genome Sequencing and Phylogenymentioning

confidence: 99%

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Hydrogen and Carbon Monoxide-Utilizing Kyrpidia spormannii Species From Pantelleria Island, Italy

et al. 2020

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Volcanic and geothermal areas are hot and often acidic environments that emit geothermal gasses, including H 2 , CO and CO 2. Geothermal gasses mix with air, creating conditions where thermoacidophilic aerobic H 2-and CO-oxidizing microorganisms could thrive. Here, we describe the isolation of two Kyrpidia spormannii strains, which can grow autotrophically by oxidizing H 2 and CO with oxygen. These strains, FAVT5 and COOX1, were isolated from the geothermal soils of the Favara Grande on Pantelleria Island, Italy. Extended physiology studies were performed with K. spormannii FAVT5, and showed that this strain grows optimally at 55 • C and pH 5.0. The highest growth rate is obtained using H 2 as energy source (µ max 0.19 ± 0.02 h −1 , doubling time 3.6 h). K. spormannii FAVT5 can additionally grow on a variety of organic substrates, including some alcohols, volatile fatty acids and amino acids. The genome of each strain encodes for two O 2-tolerant hydrogenases belonging to [NiFe] group 2a hydrogenases and transcriptome studies using K. spormannii FAVT5 showed that both hydrogenases are expressed under H 2 limiting conditions. So far no Firmicutes except K. spormannii FAVT5 have been reported to exhibit a high affinity for H 2 , with a K s of 327 ± 24 nM. The genomes of each strain encode for one putative CO dehydrogenase, belonging to Form II aerobic CO dehydrogenases. The genomic potential and physiological properties of these Kyrpidia strains seem to be quite well adapted to thrive in the harsh environmental volcanic conditions.

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Section: Growth On Other Substratesmentioning

confidence: 60%

Section: Growth On Other Substratesmentioning

confidence: 85%

Section: Genome Sequencing and Phylogenymentioning

confidence: 99%

Section: Genome Sequencing and Phylogenymentioning

confidence: 99%

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Hydrogen and Carbon Monoxide-Utilizing Kyrpidia spormannii Species From Pantelleria Island, Italy

et al. 2020

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“…The authors discuss that the reason for the absence of confluent growth might be reactive oxygen species. In recent studies, Kyrpidia spormannii was presented as a new thermoacidophilic, obligate aerobic knallgas bacterium, which is able to grow electroautotrophically and forms comparatively thick and confluent biofilms on a cathode as sole electron and energy source [ 17 , 18 ]. These findings, together with its ability to use CO 2 as a carbon source even if this is part of an unpurified flue gas, renders it an interesting new biocatalyst for microbial electrosynthesis processes [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Improving the Cathodic Biofilm Growth Capabilities of Kyrpidia spormannii EA-1 by Undirected Mutagenesis

et al. 2020

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The biotechnological usage of carbon dioxide has become a relevant aim for future processes. Microbial electrosynthesis is a rather new technique to energize biological CO2 fixation with the advantage to establish a continuous process based on a cathodic biofilm that is supplied with renewable electrical energy as electron and energy source. In this study, the recently characterized cathodic biofilm forming microorganism Kyrpidia spormannii strain EA-1 was used in an adaptive laboratory evolution experiment to enhance its cathodic biofilm growth capabilities. At the end of the experiment, the adapted cathodic population exhibited an up to fourfold higher biofilm accumulation rate, as well as faster substratum coverage and a more uniform biofilm morphology compared to the progenitor strain. Genomic variant analysis revealed a genomically heterogeneous population with genetic variations occurring to various extends throughout the community. Via the conducted analysis we identified possible targets for future genetic engineering with the aim to further optimize cathodic growth. Moreover, the results assist in elucidating the underlying processes that enable cathodic biofilm formation.

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Oxygen In The Mix: Is Oxic Microbial Electrosynthesis A Potential Alternative For Biomass Production?

Eberhard Reiner,

Korth,

Edel

2024

ChemElectroChem

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Oxic microbial electrosynthesis (oMES) allows the utilization of renewable electricity and industrial gas streams containing CO2 and O2 for biomass production by cultivating aerobic, autotrophic, hydrogen‐oxidizing bacteria, commonly known as Knallgas bacteria. oMES is likely not a direct competitor to conventional anoxic microbial electrosynthesis as harnessing aerobic hydrogen‐oxidizing bacteria depends on energetically inefficient assimilatory CO2 reduction pathways. However, it might be a complementary approach to classical biomass production from the perspective of limited land use and the availability of cheap renewable energy. The best characterized Knallgas bacterium is Cupriavidus necator. Extensively studied as lithoautotrophic production host, C. necator already offers a broad arsenal of genetic tools. In contrast, mechanistical knowledge about the recently discovered Kyrpidia spormannii is limited, but this species shows remarkable growth when cultivated as cathodic biofilm in bioelectrochemical systems. In addition, first experiments indicate a low energy demand for biomass production, which is in the order of magnitude of gas fermentation with C. necator or heterotrophic and methanotrophic technologies. Still, many aspects of the electrochemical cultivation of K. spormannii need to be better understood and rigorously improved to be a competitive technology in the making, including electron transfer and microbial kinetics, cultivation conditions, mass and energy balances, and reactor design.

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Kyrpidia spormannii sp. nov., a thermophilic, hydrogen-oxidizing, facultative autotroph, isolated from hydrothermal systems at São Miguel Island, and emended description of the genus Kyrpidia

Cited by 33 publications

References 26 publications

Hydrogen and Carbon Monoxide-Utilizing Kyrpidia spormannii Species From Pantelleria Island, Italy

Hydrogen and Carbon Monoxide-Utilizing Kyrpidia spormannii Species From Pantelleria Island, Italy

Improving the Cathodic Biofilm Growth Capabilities of Kyrpidia spormannii EA-1 by Undirected Mutagenesis

Oxygen In The Mix: Is Oxic Microbial Electrosynthesis A Potential Alternative For Biomass Production?

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