L-2-aminooxy-3-phenylpropionic acid (AOPP) Controls Browning and Promotes Adventitious Bud Formation in Neofinetia falcata in vitro

Abstract

The effect of L-2-aminooxy-3-phenylpropionic acid (AOPP) on bud formation was investigated by determination of total phenolics in explants and medium browning in the micropropagation of the epiphytic orchid Neofinetia falcata (Thunb.) H.H. Hu. Explants, upper portion of plantlets, were cultured on a medium containing 0, 0.01, 0.1 or 1.0 mM AOPP. Browning was decreased from 70% to 3% by increasing AOPP concentration up to 1.0 mM. All surviving explants developed buds at 0, 0.01 and 0.1 mM AOPP. The highest mean number of buds (7.2) was produced on medium containing 0.01 mM AOPP. Multiple buds were formed on the explants 8 weeks after planting and multiple shoots had developed after 12 weeks. Total phenolics continuously increased in control explants up to 2.03 mg·gFW −1 after 4 weeks. In contrast, in explants treated with 0.01 mM AOPP, total phenolics ceased to increase for 4 weeks of culture and remained at 1.46 mg·gFW −1 , but thereafter increased to 2.24 mg·gFW −1 , the same level as the control, after 8 weeks. Medium browning continuously increased in the control, whereas medium containing 0.01 mM AOPP showed little browning after 4 weeks of culture and thereafter increased to the same degree as the control. The present results suggest that 0.01 mM AOPP is the most suitable concentration to control browning and bud formation in N. falcata.

Developed network between taxoid and phenylpropanoid pathways in Cryptosporiopsis tarraconensis, taxan-producing endophytic fungus by Debiased Sparse Partial Correlation (DSPC) algorithm

“…It has been proved that trans -cinnamic acid reduces the activity of PAL by 40–50% [ 30 ], while reducing paclitaxel accumulation by 90% [ 31 ]. That is why in higher concentrations of BA, overproduction of cinnamic acid in C .…”

“…It has been proved that trans-cinnamic acid reduces the activity of PAL by 40-50% [30], while reducing paclitaxel accumulation by 90% [31]. That is why in higher concentrations of BA, overproduction of cinnamic acid in C. tarraconensis not only reduced the activity of PAL but also decreased Paclitaxel content to the control level.…”

Applications of Biotechnological Approaches in the Product and Breeding of Phalaenopsis Orchids

“…In fact, enzyme activities including PPO are higher in browning tissues of Phalaenopsis [52]. Therefore, activated charcoal adsorbing phenol [53,54], antioxidants such as ascorbate acid (vitamin C) [55], L-2-aminooxy-3phenylpropionic acid (AOPP, inhibitor of PAL) [56], and cycloheximide (inhibitor of PPO) [57] were added to the medium in tissue culture of Orchids. A semisynthetic Phalaenopsis Shoot Reproduction (PSR) medium was developed that relieves the effects of phenolic compounds and enhances the survival rate of the explants of Phalaenopsis [31].…”

Efficient propagation with in vitro seed germination of Vanda falcata

“…These scientists found that shoot apexes of V. falcata were the most suitable explant materials, although they exuded browning compounds into the medium (Mitsukuri et al, 2009a). Generally, two to three weeks of dark-preconditioning treatment or treatment with L-2-aminooxy-3-phenylpropionic acid (AOPP) must be carried out on shoot apexes or leaf tissue cultures to control browning (Mitsukuri et al, 2009a(Mitsukuri et al, , 2009b(Mitsukuri et al, , 2010.…”