L'acétylcholinestérase des poissons, cible des organophosphorés et des carbamates. Caractérisation du gène et des formes moléculaires de l'enzyme chez Danio rerio. Effets des anticholinestérasiques

Castro, Bertrand; Cousin, Xavier; Haubruge, Éric; Toutant, Jean‐Pierre; Chatonnet, Arnaud

doi:10.1051/kmae:1998023

Cited by 5 publications

(3 citation statements)

References 7 publications

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“…The most interesting feature was the total predominance of asymmetric forms in muscle. This observation con"rms preliminary results on zebra"sh, Brachydanio rerio (Bertrand et al, 1998), and is compatible with earlier results of Guillon and MassoulieH (1976), who found three molecular forms in gold"sh (18S, 12S, and 5S). In contrast, recent reports on carp muscles revealed a di!erent pattern of molecular forms with a minority of A12 form (15.5%) and a majority of G4/A4 form (84.5%) (Szegletes et al, 1995).…”

Section: Discussionsupporting

confidence: 92%

“…For instance, 80% inhibition in AChE activity was found in pin"sh (¸agodon rhomboides) in response to the median lethal concentration (100 g/L) of carbofuran (Coppage, 1977). In common carp, Bertrand et al (1998) observed an 80% inhibition in brain AChE activity and 26.6% lethality after a 48-h exposure to 50 g/L curater 200, a commercial form of carbofuran.…”

Section: Discussionmentioning

confidence: 94%

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Effects of Carbofuran, Diuron, and Nicosulfuron on Acetylcholinesterase Activity in Goldfish (Carassius auratus)

Bretaud¹,

Toutant²,

Saglio³

2000

Ecotoxicology and Environmental Safety

191

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 94%

Effects of Carbofuran, Diuron, and Nicosulfuron on Acetylcholinesterase Activity in Goldfish (Carassius auratus)

Bretaud¹,

Toutant²,

Saglio³

2000

Ecotoxicology and Environmental Safety

191

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“…Diafuran provoked high AChE inhibition in brain and muscle for all carps. Common carp exposed to 0.05 mg L -1 of carbofuran for 48 h showed 80% inhibition in brain AChE activity (Bertrand et al, 1998), and exposure to 50 mg L -1 in goldfish inhibited 23% AChE activity in skeletal muscle (Bretaud et al, 2000). Common carp exposed to 0.1, 0.22 mg L -1 carbofuran (99%) showed 27.8 and 75% AChE activity inhibition, respec- (Dembelé et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Acetylcholinesterase enzyme activity in carp brain and muscle after acute exposure to diafuran

Golombieski

Marchesan

Camargo

et al. 2008

Sci. agric. (Piracicaba, Braz.)

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Sublethal adverse effects may result from exposure of aquatic organisms to insecticides at environmentally relevant concentrations. Fingerlings of the common carp (Cyprinus carpio, Linnaeus, 1758), grass carp (Ctenopharyngodon idella, Valenciennes, 1844), and bighead carp (Aristichthys nobilis, Richardson, 1845) were exposed to diafuran, an insecticide widely used during rice cultivation in Southern Brazil. The aim of this study was to verify the relationship between the lethal concentration (LC 50 ) of diafuran and the acetylcholinesterase (AChE) activity in brain and muscle tissues of these species as a possible early biomarker of exposure to this insecticide. LC 50 was determined for fish exposed to diafuran concentrations during 96 h (short term): common carp: control, 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg L -1 ; grass carp: control, 1.0, 2.0, 3.0 and 3.5 mg L -1 and, bighead carp: control, 0.5, 1.0, 1.5, 2.0, 3.0 and 4.0 mg L -1 , as well as the determination of AChE at concentrations near LC 50 for these species. LC 50 values (nominal concentrations) were 1.81 mg L -1 for the common carp, 2.71 mg L -1 for the grass carp and, 2.37 mg L -1 for the bighead carp. All carps exposed to diafuran were lethargic (lower concentrations) or immobile. Diafuran inhibited the acetylcholinesterase activity in brain (~38%) and muscle (~50%) of all species. Muscle of bighead carp under control treatment showed higher specific AChE activity than brain (14.44 against 5.94 mmol min -1 g protein -1 , respectively). Concentrations of diafuran used for rice cropping may affect Cyprinus carpio, Ctenopharyngodon idella and Aristichthys nobilis behaviors and the AChE activities in brain and muscle of these species may be an early biomarker of toxicity of this insecticide. Key words: Cyprinus carpio, Ctenopharyngodon idella, Aristichthys nobilis, pesticide, lethal concentration ATIVIDADE DA ENZIMA ACETILCOLINESTERASE EM CÉREBRO E MÚSCULO DE CARPAS APÓS EXPOSIÇÃO AGUDA AO DIAFURANRESUMO: Exposição a inseticidas em concentrações elevadas no ambiente podem ocasionar efeitos adversos subletais em organismos aquáticos. Alevinos de carpa húngara (Cyprinus carpio, Linnaeus, 1758), carpa capim (Ctenopharyngodon idella, Valenciennes, 1844) e carpa cabeça grande (Aristichthys nobilis, Richardson, 1845) foram expostos ao diafuran, um inseticida utilizado na cultura do arroz no sul do Brasil. O objetivo deste estudo foi verificar a relação entre concentração letal mediana (CL 50 ) do diafuran e a atividade da enzima acetilcolinesterase (AChE) em cérebro e músculo dessas espécies, como um possível biomarcador inicial da exposição a este inseticida. A CL 50 foi determinada com peixes expostos a concentrações de diafuran em 96 h: carpa húngara: controle; 0,5; 1,0; 1,5; 2,0; 2,5 e 3,0 mg L -1 ; carpa capim: controle; 1,0; 2,0; 3,0 e 3,5 mg L -1 e carpa cabeça grande: controle; 0,5; 1,0; 1,5; 2,0; 3,0 e 4,0 mg L -1 , bem como a determinação da AChE em concentrações próximas da CL 50 para essas espécies. (5,5'dithio-bis 2 nitrobenzoic acid), bo...

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Seasonal exposure of fish to neurotoxic pesticides in an intensive agricultural catchment, Uma-oya, Sri Lanka: Linking contamination and acetylcholinesterase inhibition

Sumith

Hansani

Weeraratne

et al. 2012

Environmental Toxicology and Chemistry

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The annual cultivation pattern in the Uma-oya catchment in Sri Lanka is characterized by Yala and Maha rainfall periods and associated cropping. Two cultivation seasons were compared for pesticide residues: base flow, field drainage, and the runoff and supplementary sediment data for three sites in the catchment. Organophosphate and N-methyl carbamate pesticide analysis confirmed a higher concentration in the Yala season with low-flow conditions. Acetylcholinesterase (AChE) activity was measured by standard spectrometry in the brain, muscle, and eye tissues of three freshwater cyprinid fishes, Garra ceylonensis, Devario malabaricus, and Rasbora daniconius from three study sites during months overlapping two seasons in 2010 (December) and 2011 (July). Baseline AChE data were measured from fish samples from a forested reserve in the Knuckles. A 73% inhibition in muscle AChE activity in G. ceylonensis was associated with intense pesticide exposure months in the Yala season. The AChE inhibition more than 70% in G. ceylonensis eyes in both Yala (76%) and Maha (72.5%) seasons indicates particular sensitivity of eye tissue to inhibitors. The less dramatic AChE inhibition in the eye tissues in D. malabaricus and R. daniconius in both seasons indicates exemplary protective capacity of muscle AChE in fish. The highest inhibition of AChE (up to 60% in brain and up to 56% in muscle AChE activity in R. daniconius and up to 47.8% in brain and up to 64.6% in muscle AChE activity in D. malabaricus) occurred during the Yala season. Tissue AChE activity and physiological activity in fish were correlated. The results collectively indicate that AChE is a consistent biomarker for diffused contaminant exposure in agricultural catchments.

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L'acétylcholinestérase des poissons, cible des organophosphorés et des carbamates. Caractérisation du gène et des formes moléculaires de l'enzyme chez Danio rerio. Effets des anticholinestérasiques

Cited by 5 publications

References 7 publications

Effects of Carbofuran, Diuron, and Nicosulfuron on Acetylcholinesterase Activity in Goldfish (Carassius auratus)

Effects of Carbofuran, Diuron, and Nicosulfuron on Acetylcholinesterase Activity in Goldfish (Carassius auratus)

Acetylcholinesterase enzyme activity in carp brain and muscle after acute exposure to diafuran

Seasonal exposure of fish to neurotoxic pesticides in an intensive agricultural catchment, Uma-oya, Sri Lanka: Linking contamination and acetylcholinesterase inhibition

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