L'Antibiogramme : Méthodes classiques et Méthodes automatisées

Thabaut, A; Durosoir, J. L.

doi:10.1016/s0399-077x(79)80006-2

Cited by 2 publications

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“…Non-standardization of the inoculum could have a very important impact on the final result of the antibiotic susceptibility test, as heavy inoculum leads to smaller zone diameters and vice versa (8). Thabaut et al, (9) demonstrated that poor standardization of the inoculum resulted in a large variability in the diameters of the inhibition zones for the same bacterial strain.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the antimicrobial susceptibility testing process in clinical microbiology laboratories at Niamey, Niger

Idrissa¹,

Abdoulaye²,

Yacouba³

et al. 2021

Af J Clin Exp Micro

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Background: Risk assessment is the means of identifying and evaluating potential errors or problems that may occur in testing process. The aim of this study was to perform risk assessment of antimicrobial susceptibility testing (AST) process in clinical microbiology laboratories of Niamey, Niger Republic.Methodology: We conducted a descriptive cross-sectional study from October 1 to December 31, 2019, to evaluate AST performance in seven clinical microbiology laboratories at Niamey, the capital city of Niger republic. The evaluation focused on the determination of the criticality index (CI) of each critical point (frequency of occurrence of anomalies, severity of the process anomaly, and detectability of the anomaly during the process) in the AST process and the performance of the AST through an observation sheet using two reference strains; Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 29213.Results: The criticality index (CI) was greater than 6 for most of the critical points related to material, medium, equipment, method and labour for the AST process in all the laboratories. A range of 18-100% errors on the inhibition zone diameters of the reference strains were observed. Major and/or minor categorization (Sensitive S, Intermediate I and Resistance R) discrepancies were found at all the laboratories for either one or both reference strains. The antibiotics most affected by the S/I/R discrepancies were trimethoprim (100%), vancomycin (100%), amoxicillin (80%) and amoxicillin + clavulanic acid (70%).Conclusion: This study showed a deficiency in the control of critical control points that impacts the performance of the AST reported by the laboratories in Niger. Corrective actions are needed to improve the performance of AST in clinical microbiology laboratories in Niger. French title: Evaluation du processus de réalisation de l’antibiogramme dans les laboratoires d’analyses de biologie médicale de la ville de Niamey, Niger Contexte: L'évaluation des risques est le moyen d'identifier et d'évaluer les erreurs ou les problèmes potentiels qui peuvent survenir dans le processus de test. L'objectif de cette étude était de réaliser une évaluation des risques du processus d'antibiogramme (ABG) dans les laboratoires de microbiologie clinique de Niamey, en République du Niger.Méthodologie: Nous avons mené une étude transversale descriptive du 1er octobre au 31 décembre 2019 pour évaluer la performance des ABG dans sept laboratoires de microbiologie clinique à Niamey, capitale de la république du Niger. L'évaluation a porté sur la détermination de l'indice de criticité (IC) de chaque point critique (fréquence d'apparition des anomalies, gravité de l'anomalie du processus et détectabilité de l'anomalie au cours du processus) dans le processus et la performance des AGB à travers une fiche d'observation en utilisant deux souches de référence; Escherichia coli ATCC 25922 et Staphylococcus aureus ATCC 29213.Résultats: L'indice de criticité était supérieur à 6 pour la plupart des points critiques liés au matériel, au milieu, à l'équipement, à la méthode et à la main-d'oeuvre pour le processus AST dans tous les laboratoires. Une fourchette d'erreurs de 18 à 100% sur les diamètres des zones d'inhibition des souches de référence a été observée. Des écarts de catégorisation majeurs et/ou mineurs (Sensible: S, Intermédiaire: I et Résistance: R) ont été constatés dans tous les laboratoires pour l'une ou les deux souches de référence. Les antibiotiques les plus touchés par les écarts S/I/R étaient la triméthoprime (100%), la vancomycine (100%), l'amoxicilline (80%) et l'amoxicilline + acide clavulanique (70%).Conclusion: Cette étude a montré une déficience dans le contrôle des points de contrôle critiques qui a un impact sur la performance de l'antibiogramme rapportée par les laboratoires au Niger. Des actions correctives sont nécessaires pour améliorer la performance des ABG dans les laboratoires de microbiologie clinique au Niger.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the antimicrobial susceptibility testing process in clinical microbiology laboratories at Niamey, Niger

Idrissa¹,

Abdoulaye²,

Yacouba³

et al. 2021

Af J Clin Exp Micro

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“…Antimicrobial tests of synthesized compounds 2a-e and 6a-e were evaluated against Pseudomonas aeruginosa and Escherichia coli (Gram-negative bacteria) and then against Staphyloccocus aureus and Enterococcus faecalis (Gram-positive bacteria). The method used was the liquid medium micromethod performed in a Open Journal of Medicinal Chemistry 96-well plate [18]. The compounds were dissolved in sterile DMSO so that their concentration in the wells of column 12 was 256 µg/mL followed by a dilution range of order 2 in DMSO to obtain a concentration series of the sample solution (from 256 to 0.063 µg/mL).…”

Section: Antibacterial Assaymentioning

confidence: 99%

Design, Synthesis and Antibacterial Activity Evaluation of 4,5-Diphenyl-1<i>H</i>-Imidazoles Derivatives

Bamoro¹,

Bamba²,

Steve-Evanes³

et al. 2021

OJMC

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Due to the continuous emergence and rapid spread of drug-resistant strains of bacteria, there is an urgent need for the development of novel antimicrobials. Along this line, the synthesis and antibacterial activity of 4,5-diphenylimidazol-2-thiol derivatives 2a-g and 6a-e are reported. The structures of the synthesized compounds were confirmed by Nuclear Magnetic Resonance (NMR) and High Resolution Mass Spectrometry (HRMS). All compounds were screened in vitro for their antibacterial activity against Pseudomonas aeruginosa and Escherichia coli (Gram-negative bacteria) and also against Staphyloccocus aureus and Enterococcus faecalis (Gram-positive bacteria).The results showed most of the synthesized compounds have no antibacterial activity. However compound 6d was two-fold potent than ciprofloxacin against Staphylococcus aureus with Minimum Inhibitory Concentration (MIC) of 4 µg/mL and 6c showed moderate biological activity against Staphylococcus aureus (16 µg/mL) and Enterococcus faecalis (16 µg/mL).

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L'Antibiogramme : Méthodes classiques et Méthodes automatisées

Cited by 2 publications

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Evaluation of the antimicrobial susceptibility testing process in clinical microbiology laboratories at Niamey, Niger

Evaluation of the antimicrobial susceptibility testing process in clinical microbiology laboratories at Niamey, Niger

Design, Synthesis and Antibacterial Activity Evaluation of 4,5-Diphenyl-1<i>H</i>-Imidazoles Derivatives

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L'Antibiogramme : Méthodes classiques et Méthodes automatisées

Cited by 2 publications

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Evaluation of the antimicrobial susceptibility testing process in clinical microbiology laboratories at Niamey, Niger

Evaluation of the antimicrobial susceptibility testing process in clinical microbiology laboratories at Niamey, Niger

Design, Synthesis and Antibacterial Activity Evaluation of 4,5-Diphenyl-1&lt;i&gt;H&lt;/i&gt;-Imidazoles Derivatives

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Design, Synthesis and Antibacterial Activity Evaluation of 4,5-Diphenyl-1<i>H</i>-Imidazoles Derivatives