L-arginine, a nitric oxide precursor, reduces dapsone-induced methemoglobinemia in rats

Moraes, Natália Valadares de; Bergamaschi, Mateus M.; Bianchi, María de Lourdes Pires; Bragheto, Juliana Bordinassi; Malfará, Wilson Roberto; Queiróz, Regina Helena Costa

doi:10.1590/s1984-82502012000100010

Cited by 3 publications

(3 citation statements)

References 41 publications

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“…Since Tendoflex ® contains more than one active ingredient, the amount to be given to the rats was decided by checking these active ingredients separately. [12][13][14][15][16] Bromelain, L-arginine, and methyl sulfonyl methane were used as reference for the dose calibration according to the whole capsule. In a study on bromelain, a dose of 30 mg/kg was shown to be beneficial on tendon damage.…”

Section: Discussionmentioning

confidence: 99%

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The effects of Tendoflex® (polytendon complex) and Hypericum perforatum (St. John’s wort oil) on repaired Achilles tendon healing in rats

et al. 2021

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Objectives This experimental study aims to examine the effects of Tendoflex® and Hypericum perforatum on tendon healing in rat models undergoing iatrogenic Achilles tendon rupture and similar surgical treatments. Materials and methods Eighty Wistar albino rats weighing 250 to 350 g were randomly divided into four groups. Group A: Tendoflex® was administered orally as 1 capsule/2.5 kg daily by gavage. Group B: Hypericum perforatum was administered orally as 300 mg/kg daily by gavage. Group C: Tendoflex® and Hypericum perforatum were co-administered orally by gavage at the prespecified doses. Group D: No medication was given to the control group. Five rats from each group were sacrificed weekly, and the tissue samples were examined histologically, followed by the biomechanical tests of the Achilles tendon. Results In the mechanical testing, pulling forces were superior in all intervention groups and in all weeks over the control group. In particular, in the early periods (Weeks 1, 2, and 3), the mixed group showed the most favorable results, followed by the Hypericum perforatum group (p=0.010, p=0.591, and p=0.130, respectively). The most favorable collagen type I and type III expression values were found in the mixed and Hypericum perforatum groups at Weeks 2 and 3, respectively (p=0.025 and p=0.018). In the immunohistochemical and Western Blot examinations, extreme collagen type I and type III expression were detected in the mixed and Hypericum perforatum groups at Weeks 2, 3, and 4. Conclusion Tensile strength of the Achilles tendon increased by using Hypericum perforatum and Tendoflex® following rupture and repair of the Achilles tendon in rats. The combined use of these two agents yielded the most favorable mechanical and histological results, particularly in the early period. This result may be related to the higher level of collagen type I and type III immunity in all groups, compared to the control group.

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Section: Discussionmentioning

confidence: 99%

“…Again, in another study, L-arginine was used in rats at a dose of 30 to 60 mg/kg. [ 14 ] In another study, methyl sulfonyl methane was used at a dose of 50 mg/kg. [ 13 ] One tablet containing 125 mg of methyl sulfonyl methane was used for each 2.5 kg of a rat to use 50 mg of methyl sulfonyl methane per kg.…”

Section: Discussionmentioning

confidence: 99%

The effects of Tendoflex® (polytendon complex) and Hypericum perforatum (St. John’s wort oil) on repaired Achilles tendon healing in rats

et al. 2021

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“…This effect is mediated by nitric oxide formation, since the reduction in methemoglobin levels by L-arginine is blocked by simultaneous administration with L-NAME, a nitric oxide synthase inhibitor 20 . It is possible that an analysis of the effects of pelvic radiation in prostate rats made over longer periods than that used in this study, 21 days, could result in more drastic changes than those observed in this work.…”

Section: Discussionmentioning

confidence: 99%

Nutritional supplementation with arginine protects radiation-induced effects. An experimental study

et al. 2016

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PURPOSE:To investigate the protective effect of L-arginine on the prostate (nonneoplasic) of rats with radiation-induced injury. METHODS:Twenty-nine Wistar rats, male adult, allocated into three groups: Control group (C) was not exposed to irradiation (n=10); Radiated group (R) had undergone pelvic irradiation (n=10); Supplemented and radiated group (R+S) had undergone pelvic irradiation plus L-arginine supplementation (n=9). The animals were observed for signs of toxicity. After euthanization, the prostate was dissected under magnification and stained by hematoxylin and eosin to study acinar structures and stained with Picrosirius red for collagen analysis. RESULTS:After radiation exposure, all animals presented diarrhea, but supplementation with L-arginine reduced this effect. The weight gain in the R+S group was significantly higher than in the C and R groups. In the R+S group the collagen density and the prostate acinar area was similar to the R and C groups. Epithelial height was significantly reduced in group R compared with group C (p<0.0001). When comparing the group R+S with R, a statistical difference was observed to be present (p<0.0001). CONCLUSIONS:Pelvic radiation promotes systemic effects and some structural modifications in the ventral prostate of rats. These modifications can be prevented by oral supplementation with L-arginine.

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L–arginine alleviates postmenopausal complications in female rats by stimulating ovarian dopamine beta hydroxylase

Karami

Lakzaei

Nadoushan

2022

Asian Pacific Journal of Reproduction

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Objective: To evaluate the levels of estrogen, albumin and gonadotropins (luteinizing hormone, follicle-stimulating hormone) as well as the activity of dopamine beta hydroxylase (DAß H) in aged female rats treated with nitric oxide precursor L-arginine and neuronal nitric oxide synthase antagonist L-NAME. Methods: A total of 224 Wistar rats (36 weeks old, weighing 250 g) based on a random sampling were divided into the control and experimental groups after Pap smear test. The control group received only saline (1 mL/kg) intraperitoneally (i.p.). The experiential groups were treated with L-arginine (5, 25 and 50 mg/kg, i.p.) and L-NAME (5 and 25 mg/kg, i.p.) for 3 to 21 days, once a day. Blood samples were taken from the rats and the levels of estrogen and albumin and gonadotropins in the serum were monitored by enzyme-linked immunosorbent assay kit, and the ovaries were examined immunohistopathologically for DAßH activity. Results: L-arginine (5 mg/kg) significantly increased estrogen level (P<0.05), which was associated with DAßH activation in the ovaries. L-NAME reduced this effect when administered prior to L-arginine dose. L-arginine caused no significant change in the levels of luteinizing hormone and follicle-stimulating hormone. Except for the lowest dose of L-arginine in the shortest period, albumin levels significantly decreased in other treatments compared to the control group (P<0.05). Conclusions: L-arginine is likely to reduce postmenopausal problems due to an increased nitric oxide level.

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L-arginine, a nitric oxide precursor, reduces dapsone-induced methemoglobinemia in rats

Cited by 3 publications

References 41 publications

The effects of Tendoflex® (polytendon complex) and Hypericum perforatum (St. John’s wort oil) on repaired Achilles tendon healing in rats

The effects of Tendoflex® (polytendon complex) and Hypericum perforatum (St. John’s wort oil) on repaired Achilles tendon healing in rats

Nutritional supplementation with arginine protects radiation-induced effects. An experimental study

L–arginine alleviates postmenopausal complications in female rats by stimulating ovarian dopamine beta hydroxylase

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