L-arginine affects the IVM of cattle cumulus-oocyte complexes

Dubeibe, D. F.; Caldas-Bussiere, M.C.; Maciel, Valter Luiz; Sampaio, Wlaisa Vasconcelos; Quirino, C. R.; Gonçalves, Paulo Bayard Dias; Cesaro, Matheus Pedrotti de; Faes, M.R.; Carvalho, Carla Sobrinho Paes de

doi:10.1016/j.theriogenology.2016.09.017

Cited by 18 publications

(16 citation statements)

References 49 publications

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“…These alterations in nucleotide concentrations are different from those observed in rodents (Tiwari and Chaube, 2017). However, we and others (Schwarz et al, 2014;Botigelli et al, 2017;Dubeibe et al, 2017) have also observed the lack of correlation between cGMP and cAMP concentration during meiosis resumption in bovines. Recently, using this same experimental model, we showed that this increase in cAMP in the COC, which we also observed here, is due to increase of the production by CCs, causing a crescent decreasing in its concentration in the oocyte (Torres et al, 2015).…”

Section: Nos Inhibition In Bovine Ivmcontrasting

confidence: 71%

“…Two of these isoforms are constitutive (NOS1 or neuronal nNOS and NOS3 or endothelial eNOS), while the third is inducible (NOS2 or iNOS) (Stuehr et al, 2004). However, the specific involvement of the NOS/NO system in events that regulate bovine oocyte maturation has not been entirely elucidated (Viana et al, 2007;Matta et al, 2009;Schwarz et al, 2014;Botigelli et al, 2017;Dubeibe et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Partial inhibition of nitric oxide synthase activity stimulates the nuclear maturation progression of bovine cumulus–oocyte complex in vitro in the presence of hemisections of the follicular walls

Marin

Caldas-Bussiere

Maciel

et al. 2020

Zygote

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Summary This study aimed to assess the effects of the inhibition of nitric oxide synthase (NOS) on events that modulate bovine in vitro oocyte maturation. Cumulus–oocyte complexes (COCs) were cultured with hemisections (HSs) of the follicular walls in a maturation medium supplemented with different concentrations (0.1–10.0 mM) of Nω-nitro-l-arginine methyl ester hydrochloride (l-NAME). Controls consisted of COCs cultured in the presence (+HSs) or absence of HSs (–HSs) with no additional l-NAME supplementation. The following parameters were assessed: oocyte nuclear maturation stage; cumulus cell (CC) membrane integrity; nitrate/nitrite, progesterone, and estradiol concentrations in the culture medium at 22 h of cultivation; and the concentrations of cGMP and cAMP in COCs during the first hour of maturation. The addition of 1.0 mM l-NAME increased the percentage of oocytes that reached metaphase II (MII) and the percentage of intact CCs (P < 0.05). All l-NAME concentrations reduced the nitrate/nitrite concentrations (P < 0.05), but none affected steroid concentrations compared with control +HSs (P > 0.05). The addition of 1.0 mM l-NAME reduced cGMP concentrations at 3 h and increased cAMP concentrations in the first hour of culture (P < 0.05). Our findings suggest that the NOS/NO/cGMP pathway participates in meiosis progression (MI to MII) of the bovine oocytes matured in vitro in the presence of hemisections of the follicular walls. Lastly, the mechanisms that lead to the progression of meiosis after NOS inhibition do not involve changes in steroid production.

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Section: Nos Inhibition In Bovine Ivmcontrasting

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Partial inhibition of nitric oxide synthase activity stimulates the nuclear maturation progression of bovine cumulus–oocyte complex in vitro in the presence of hemisections of the follicular walls

Marin

Caldas-Bussiere

Maciel

et al. 2020

Zygote

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“…This lack of increase in cAMP after treatments that would increase cGMP was also reported in other studies (Bilodeau-Goeseels, 2007; Schwarz et al ., 2014). Dubeibe et al (2017) also showed that nucleotides had opposite and not parallel levels in bovine COCs. Using a different strategy, they reported that COCs matured in the presence of follicle walls with or without l -arginine, the precursor for NO synthesis, cGMP levels declined, while cAMP levels increased.…”

Section: Discussionmentioning

confidence: 97%

Influence of nitric oxide and phosphodiesterases duringin vitromaturation of bovine oocytes on meiotic resumption and embryo production

Botigelli

Schwarz

Zaffalon

et al. 2017

Zygote

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This study aimed to examine the effects of nitric oxide (NO) and different phosphodiesterase (PDE) families on meiosis resumption, nucleotides levels and embryo production. Experiment I, COCs were matured in vitro with the NO donor S-nitroso-N-acetylpenicillamine (SNAP) associated or not with the soluble guanylate cyclase (sGC) inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), meiotic resumption and nucleotides levels were assessed. SNAP delayed germinal vesicle breakdown (GVBD) (53.4 ± 1.2 versus 78.4 ± 2.4% for controls, P 0.05). Cyclic GMP levels were higher in SNAP (3.94 ± 0.18, P 0.05). Embryo development did not differ from the control for SNAP and cilostamide groups (38.7 ± 5.8, 37.9 ± 6.2 and 40.5 ± 5.8%, P > 0.05), but SNAP + cilostamide decreased embryo production (25.7 ± 6.9%, P < 0.05). In conclusion, SNAP was confirmed to delay meiosis resumption by the NO/sGC/cGMP pathway, by increasing cGMP, but not cAMP. Inhibiting different PDEs to further increase nucleotides in association with SNAP did not show any additive effects on meiosis resumption, indicating that other pathways are involved. Moreover, SNAP + cilostamide affected the meiosis progression and decreased embryo development.

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“…The LCE are asymptotic measures characterizing the average rate of growth (or shrinking) of small perturbations along the solutions of a dynamical system. The concept was introduced by Lyapunov when studying the stability of non-stationary solutions of ordinary differential equations [30][31][32][33][39][40][41] and has been widely employed in dynamical systems since then.…”

Section: Appendix A: Lyapunov Characteristic Exponent (Lce)mentioning

confidence: 99%

Classical chaos in atom-field systems

et al. 2016

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The relation between the onset of chaos and critical phenomena, like quantum phase transitions (QPTs) and excited-state quantum phase transitions (ESQPTs), is analyzed for atom-field systems. While it has been speculated that the onset of hard chaos is associated with ESQPTs based in the resonant case, the off-resonant cases, and a close look at the vicinity of the QPTs in resonance, show clearly that both phenomena, ESQPTs and chaos, respond to different mechanisms. The results are supported in a detailed numerical study of the dynamics of the semiclassical Hamiltonian of the Dicke model. The appearance of chaos is quantified calculating the largest Lyapunov exponent for a wide sample of initial conditions in the whole available phase space for a given energy. The percentage of the available phase space with chaotic trajectories is evaluated as a function of energy and coupling between the qubit and bosonic part, allowing us to obtain maps in the space of coupling and energy, where ergodic properties are observed in the model. Different sets of Hamiltonian parameters are considered, including resonant and off-resonant cases.

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L-arginine affects the IVM of cattle cumulus-oocyte complexes

Cited by 18 publications

References 49 publications

Partial inhibition of nitric oxide synthase activity stimulates the nuclear maturation progression of bovine cumulus–oocyte complex in vitro in the presence of hemisections of the follicular walls

Partial inhibition of nitric oxide synthase activity stimulates the nuclear maturation progression of bovine cumulus–oocyte complex in vitro in the presence of hemisections of the follicular walls

Influence of nitric oxide and phosphodiesterases duringin vitromaturation of bovine oocytes on meiotic resumption and embryo production

Classical chaos in atom-field systems

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