L-arginine and N-carbamoylglutamic acid supplementation enhance young rabbit growth and immunity by regulating intestinal microbial community

Sun, Xiaoming; Shen, Jinglin; Liu, Chang; Li, Sheng; Peng, Chunyi; Chen, Chengzhen; Yuan, Bao; Gao, Yan; Meng, Xianmei; Jiang, Hao

doi:10.5713/ajas.18.0984

Cited by 17 publications

(16 citation statements)

References 31 publications

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“…In this study, we found at the genus level, high red meat intake significantly increased the relative abundance of Bacteroides and Alistipes genera in colon, but reduced the abundance of Lachnospiraceae_NK4A136 group, Faecalibaculum, Blautia, and Dubosiella genera. Previous studies have shown that the genera of Bacteroides and Alistipes can affect amino acid transport, metabolism, energy production and transport, and inflammation (34)(35)(36). Bacteroides can secret metalloprotease toxin both in vivo and in vitro (37), which stimulate the cleavage of intercellular adhesion protein E-cadherin in colonic epithelial cells, resulting in increased colonic mucosal permeability and activating NF-kB pathway.…”

Section: Discussionmentioning

confidence: 99%

High Red Meat Intake Exacerbates Dextran Sulfate-Induced Colitis by Altering Gut Microbiota in Mice

Cui

Tan

et al. 2021

Front. Nutr.

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Inflammatory bowel disease (IBD) is a serious hazard to public health, but the precise etiology of the disease is unclear. High intake of red meat diet is closely related to the occurrence of IBD. In this study, we investigated whether the high intake of red meat can increase the sensitivity of colitis and the underlying mechanism. Mice were fed with different levels of red meat for 8 weeks and then the colonic contents were analyzed by 16S rRNA sequencing. Then 3% dextran sulfate sodium was used to induce colitis in mice. We observed the severity of colitis and inflammatory cytokines. We found that high-dose red meat caused intestinal microbiota disorder, reduced the relative abundance of Lachnospiraceae_NK4A136_group, Faecalibaculum, Blautia and Dubosiella, and increased the relative abundance of Bacteroides and Alistipes. This in turn leads to an increase in colitis and inflammatory cytokine secretion. Moreover, we found that high red meat intake impaired the colon barrier integrity and decreased the expression of ZO-1, claudin, and occludin. We also found high red meat intake induced the production of more inflammatory cytokines such as IL-1β, TNF-α, IL-17, and IL-6 and inflammatory inducible enzymes such as COX-2 and iNOS in dextran sulfate sodium-induced colitis. These results suggest that we should optimize the diet and reduce the intake of red meat to prevent the occurrence of IBD.

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Section: Discussionmentioning

confidence: 99%

High Red Meat Intake Exacerbates Dextran Sulfate-Induced Colitis by Altering Gut Microbiota in Mice

Cui

Tan

et al. 2021

Front. Nutr.

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“…The metagenome-assembled genomes of Mollicutes_RF9 group also have the CAZyme related with starch degradation, thus lower concentration of undigested starch flowing into the cecum might limit the growth of Mollicutes_RF9 (Ma et al, 2019) produce butyrate; in addition, genus norank_f_Lachnospiraceae, Shuttleworthia, and Ruminococcus_torques_group have been observed to be related to better performance (Torok et al, 2011;Lee et al, 2017;McCafferty et al, 2019a;Li et al, 2020). Genera (Hydrogenoanaerobacterium and Ruminococcaceae_V9D2013_group) from the phylum Firmicutes are also suggested to effectively produce butyrate (Fang et al, 2020;Ilaria et al, 2020;Sun et al, 2020). Genus unclassified_f_Ruminococcaceae, Anaerotruncus, Caproiciproducens, and Subdoligranulum (family Ruminococcaceae) are also known as short-chain fatty acid producers.…”

Section: Discussionmentioning

confidence: 99%

Effects of Xylanase in Corn- or Wheat-Based Diets on Cecal Microbiota of Broilers

et al. 2021

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Xylanase has been demonstrated to improve growth performance of broilers fed wheat- or corn-based diets due to its ability to degrade arabinoxylans (AX). However, content and structure of AX in corn and wheat are different, comparing effects of xylanase on cecal microbiota of broilers fed corn- or wheat-based diets could further elaborate the mechanism of the specificity of xylanase for different cereal grains. Thus, a total of 192 one-day-old broilers were randomly allotted into four dietary treatments, including wheat-soybean basal diet, wheat-soybean basal diet with 4,000U/kg xylanase, corn-soybean basal diet, and corn-soybean basal diet with 4,000U/kg xylanase to evaluate interactive effects of xylanase in corn- or wheat-based diets on broilers cecal microbiota during a 6-week production period. The results indicated that bacterial community clustering was mainly due to cereal grains rather than xylanase supplementation. Compared with broilers fed wheat-based diets, corn-based diets increased alpha-diversity and separated from wheat-based diets (p<0.05). Xylanase modulated the abundance of specific bacteria without changing overall microbial structure. In broilers fed wheat-based diets, xylanase increased the abundance of Lactobacillus, Bifidobacterium, and some butyrate-producing bacteria, and decreased the abundance of non-starch polysaccharides-degrading (NSP) bacteria, such as Ruminococcaceae and Bacteroidetes (p<0.05). In broilers fed corn-based diets, xylanase decreased the abundance of harmful bacteria (such as genus Faecalitalea and Escherichia-Shigella) and promoted the abundance of beneficial bacteria (such as Anaerofustis and Lachnospiraceae_UCG_010) in the cecum (p<0.05). Overall, xylanase supplementation to wheat- or corn-based diets improved broilers performance and cecal microbiota composition. Xylanase supplementation to wheat-based diets increased the abundance of butyrate-producing bacteria and decreased the abundance of NSP-degrading bacteria. Moreover, positive effects of xylanase on cecal microbiota of broilers fed corn-based diets were mostly related to the inhibition of potentially pathogenic bacteria, and xylanase supplementation to corn-based diets slightly affected the abundance of butyrate-producing bacteria and NSP-degrading bacterium, the difference might be related to lower content of AX in corn compared to wheat.

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“…Unexpectedly, the previous study indicated that the growth of Lactobacillus was dependent on the Arg to a great extent [33], and the lactic acid produced by Lactobacillus could be further used as substrate by butyrateproducing bacteria such as Ruminococcaceae [36]. In addition, Arg can modulate the metabolism of the Arg, serine and aspartate-family of amino acids in intestinal bacteria and decrease the utilization of most amino acids in mixed bacteria [37]. These may partly explain why Arg can stimulated probiotics and affected the microbiota.…”

Section: Discussionmentioning

confidence: 99%

Dynamic alterations in early intestinal development, microbiota and metabolome induced by in ovo feeding of L-arginine in a layer chick model

Dai¹,

Wu²,

Zhang³

et al. 2020

J Animal Sci Biotechnol

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Background: Prenatal nutrition is crucial for embryonic development and neonatal growth, and has the potential to be a main determinant of lifelong health. In the present study, we used a layer chick model to investigate the effects of in ovo feeding (IOF) of L-arginine (Arg) on growth, intestinal development, intestinal microbiota and metabolism. The treatments included the non-injected control, saline-injected control, and saline containing 2, 6, or 10 mg Arg groups. Results: IOF Arg increased early intestinal index and villus height, and enhanced uptake of residual yolk lipid, contributing to subsequent improvement in the early growth performance of chicks. Prenatal Arg supplementation also increased the early microbial α-diversity, the relative abundance of Lactobacillales and Clostridiales, and decreased the relative abundance of Proteobacteria of cecum in chicks. Furthermore, the shift of cecal microbiota composition and the colonization of potential probiotics were accelerated by IOF of Arg. Simultaneously, metabolomics showed that metabolisms of galactose, taurine-conjugated bile acids and lipids were modulated to direct more energy and nutrients towards rapid growth of intestine at the beginning of post-hatch when embryos received IOF of Arg. Conclusions: Prenatal Arg supplementation showed beneficial effects on the early intestinal development, cecal microbiota and host metabolism of layer chicks, contributing to subsequent improvement in the early growth performance. These findings provide new insight into the role of IOF of Arg in the establishment of the gut microbiota of newly-hatched layer chicks, and can expand our fundamental knowledge about prenatal nutrition, early bacterial colonization and intestinal development in neonate.

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L-arginine and N-carbamoylglutamic acid supplementation enhance young rabbit growth and immunity by regulating intestinal microbial community

Cited by 17 publications

References 31 publications

High Red Meat Intake Exacerbates Dextran Sulfate-Induced Colitis by Altering Gut Microbiota in Mice

High Red Meat Intake Exacerbates Dextran Sulfate-Induced Colitis by Altering Gut Microbiota in Mice

Effects of Xylanase in Corn- or Wheat-Based Diets on Cecal Microbiota of Broilers

Dynamic alterations in early intestinal development, microbiota and metabolome induced by in ovo feeding of L-arginine in a layer chick model

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