L-Arginine enhanced perylenequinone production in the endophytic fungus Shiraia sp. Slf14(w) via NO signaling pathway

Chen, Yunni; Yang, Huilin; Liu, Zhenying; Zhang, Zhibin; Ren, Yan; Zhu, Du

doi:10.1007/s00253-022-11877-3

Cited by 13 publications

(16 citation statements)

References 74 publications

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“…The biotechnological production of perylenequinone in Shiraia mycelium culture is becoming a promising alternative to the chemical extraction from the wild fruiting bodies. Recently, various strategies have been applied to promote perylenequinone production, including cultural medium optimization [ 31 , 32 ], using microbial or abiotic elicitors (La 3+ , surfactant treatment, light and ultrasound stimulation) [ 16 , 17 , 33 ]. Although nitrogen sources including amino acids are required for Shiraia growth and development [ 34 ], few studies have paid attention to the regulatory effects of amino acids on the fungal secondary metabolites.…”

Section: Discussionmentioning

confidence: 99%

“…Although nitrogen sources including amino acids are required for Shiraia growth and development [ 34 ], few studies have paid attention to the regulatory effects of amino acids on the fungal secondary metabolites. Recently, Chen et al [ 35 ] reported that L‑arginine at 7 g/L could increase perylenequinone yield of Shiraia sp. Slf14 and its natural mutant Shiraia sp.…”

Section: Discussionmentioning

confidence: 99%

“…Slf14 and its natural mutant Shiraia sp. Slf14 (w) by 1.51- and 30.52-fold respectively [ 35 ]. BCAAs were used to enhance the production of macrocyclic polyketide antibiotic in Streptomyces [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

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Effects of branched-chain amino acids on Shiraia perylenequinone production in mycelium cultures

Shen

Cong

et al. 2023

Microb Cell Fact

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Background Perylenequinones from Shiraia fruiting bodies are excellent photosensitizers and widely used for anti-cancer photodynamic therapy (PDT). The lower yield of Shiraia perylenequinones becomes a significant bottleneck for their medical application. Branched-chain amino acids (BCAAs) not only serve as important precursors for protein synthesis, but also are involved in signaling pathway in cell growth and development. However, there are few reports concerning their regulation of fungal secondary metabolism. In present study, the eliciting effects of BCAAs including l-isoleucine (l-Ile), l-leucine (l-Leu) and l-valine (l-Val) on Shiraia perylenequinone production were investigated. Results Based on the analysis of the transcriptome and amino acid contents of Shiraia in the production medium, we revealed the involvement of BCAAs in perylenequinone biosynthesis. The fungal conidiation was promoted by l-Val treatment at 1.5 g/L, but inhibited by l-Leu. The spore germination was promoted by both. The production of fungal perylenequinones including hypocrellins A (HA), HC and elsinochromes A-C (EA–EC) was stimulated significantly by l-Val at 1.5 g/L, but sharply suppressed by l-Leu. After l-Val treatment (1.5 g/L) in Shiraia mycelium cultures, HA, one of the main bioactive perylenequinones reached highest production 237.92 mg/L, about 2.12-fold than that of the control. Simultaneously, we found that the expression levels of key genes involved in the central carbon metabolism and in the late steps for perylenequinone biosynthesis were up-regulated significantly by l-Val, but most of them were down-regulated by l-Leu. Conclusions Our transcriptome analysis demonstrated that BCAA metabolism was involved in Shiraia perylenequinone biosynthesis. Exogenous BCAAs exhibit contrasting effects on Shiraia growth and perylenequinones production. l-Val could promote perylenequinone biosynthesis via not only enhancing the central carbon metabolism for more precursors, but also eliciting perylenequinone biosynthetic gene expressions. This is the first report on the regulation of BCAAs on fungal perylenequinone production. These findings provided a basis for understanding physiological roles of BCAAs and a new avenue for increasing perylenequinone production in Shiraia mycelium cultures. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Effects of branched-chain amino acids on Shiraia perylenequinone production in mycelium cultures

Shen

Cong

et al. 2023

Microb Cell Fact

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“…Finally, each 250-mL conical flasks containing 70 mL of medium and 4 mL of homogeneous mycelium seed broth was placed in a shaker (Hu et al 2012 ). The broth was cultured for 8 days as previously mentioned (Chen et al 2022 ; Xu et al 2023 ).…”

Section: Methodsmentioning

confidence: 99%

“…during SmF. These factors encompass variables like light exposure, carbon and nitrogen sources, amino acids, inducers, temperature, and even genetic modifications aimed at achieving optimal PQ yield (Chen et al 2022 ; Liu et al 2018a ; Xu et al 2023 ; Yan et al 2014 ; Yang et al 2009 ). As a result, to achieve substantial gains in PQ production, the imperative task of delving into SmF techniques for Shiraia spp.…”

Section: Introductionmentioning

confidence: 99%

Heat stress-induced NO enhanced perylenequinone biosynthesis of Shiraia sp. via calcium signaling pathway

Bao,

Chen,

Zhang

et al. 2024

Appl Microbiol Biotechnol

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Perylenequinones (PQs) are natural photosensitizing compounds used as photodynamic therapy, and heat stress (HS) is the main limiting factor of mycelial growth and secondary metabolism of fungi. This study aimed to unravel the impact of HS-induced Ca2+ and the calcium signaling pathway on PQ biosynthesis of Shiraia sp. Slf14(w). Meanwhile, the intricate interplay between HS-induced NO and Ca2+ and the calcium signaling pathway was investigated. The outcomes disclosed that Ca2+ and the calcium signaling pathway activated by HS could effectively enhance the production of PQs in Shiraia sp. Slf14(w). Further investigations elucidated the specific mechanism through which NO signaling molecules induced by HS act upon the Ca2+/CaM (calmodulin) signaling pathway, thus propelling PQ biosynthesis in Shiraia sp. Slf14(w). This was substantiated by decoding the downstream positioning of the CaM/CaN (calcineurin) pathway in relation to NO through comprehensive analyses encompassing transcript levels, enzyme assays, and the introduction of chemical agents. Concurrently, the engagement of Ca2+ and the calcium signaling pathway in heat shock signaling was also evidenced. The implications of our study underscore the pivotal role of HS-induced Ca2+ and the calcium signaling pathway, which not only participate in heat shock signal transduction but also play an instrumental role in promoting PQ biosynthesis. Consequently, our study not only enriches our comprehension of the mechanisms driving HS signaling transduction in fungi but also offers novel insights into the PQ synthesis paradigm within Shiraia sp. Slf14(w). Key points • The calcium signaling pathway was proposed to participate in PQ biosynthesis under HS. • HS-induced NO was revealed to act upon the calcium signaling pathway for the first time.

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Heat stress enhanced perylenequinones biosynthesis of Shiraia sp. Slf14(w) through nitric oxide formation

Lin

Chen

Gao

et al. 2023

Appl Microbiol Biotechnol

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L-Arginine enhanced perylenequinone production in the endophytic fungus Shiraia sp. Slf14(w) via NO signaling pathway

Cited by 13 publications

References 74 publications

Effects of branched-chain amino acids on Shiraia perylenequinone production in mycelium cultures

Effects of branched-chain amino acids on Shiraia perylenequinone production in mycelium cultures

Heat stress-induced NO enhanced perylenequinone biosynthesis of Shiraia sp. via calcium signaling pathway

Heat stress enhanced perylenequinones biosynthesis of Shiraia sp. Slf14(w) through nitric oxide formation

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