L-Arginine Enhances Intracellular Killing of Carbapenem-Resistant Klebsiella pneumoniae ST258 by Murine Neutrophils

Peñaloza, Hernán F.; Ahn, Danielle; Schultz, Bárbara M.; Piña-Iturbe, Alejandro; González, Liliana; Bueno, Susan M.

doi:10.3389/fcimb.2020.571771

Cited by 10 publications

(12 citation statements)

References 56 publications

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“… 33 Furthermore, the researchers found L-Arginine Enhances Intracellular killing of carbapenem-resistant Klebsiella pneumoniae ST258 by murine neutrophils. 34 Therefore, we speculate that arginine and proline metabolism play a critical role in the pathogenesis of K. pneumoniae . However, there is an obvious a shortcoming of the work that is the lack of full genome sequencing data for the two lineages.…”

Section: Discussionmentioning

confidence: 91%

Two Phenotypes of Klebsiella pneumoniae ST147 Outbreak from Neonatal Sepsis with a Slight Increase in Virulence

Zou¹,

Shen²,

Li³

et al. 2022

IDR

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Severe infection has been the leading causes of neonatal death, especially the emergency of multidrug-resistant bacteria such as carbapenem-resistant Enterobacteriaceae. This study aimed to investigate the outbreak of carbapenem-resistant Klebsiella pneumoniae (CR-KP) in neonatal wards and to explore the possible pathogenesis of Klebsiella pneumoniae. Materials and Methods: CR-KP were collected from neonatal ward of Chongqing Health Center for Women and Children between 2017 and 2019. Broth microdilution method was used to evaluate the antimicrobial activities in vitro, at the same time, the virulence of the strain was evaluated by in vitro and in vivo experiments. At last, prokaryotic chain specific transcriptome sequencing was conducted to explore the possible pathogenesis of CR-KP. Results: In this study, a total of 14 carbapenem-resistant-Klebsiella pneumoniae (CR-KP) strains were isolated from Chongqing Health Center for Women and Children, among which all CR-KP isolates were identified as NDM-1-producers. Molecular epidemiological studies revealed ST147 being the most common sequence type (ST). Moreover, we first found two phenotypes of K. pneumoniae with different virulence from the same specimen. Type I, which was a white and sticky colony had a slight increase in virulence with higher biofilm formation, serum resistance and virulence than Type II with gray colony. Compared with the Type II, 10 pathways were obviously changed in Type I especially amino acid metabolism, such as arginine and proline metabolism. Conclusion:Our findings revealed a new potential threat of NDM-1-positive CR-KP with higher virulence in neonatal ICU ward. We found two phenotypes of K. pneumoniae with different virulent, which may be due to the difference expression of arginine and proline metabolism.

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Section: Discussionmentioning

confidence: 91%

Two Phenotypes of Klebsiella pneumoniae ST147 Outbreak from Neonatal Sepsis with a Slight Increase in Virulence

Zou¹,

Shen²,

Li³

et al. 2022

IDR

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“…For instance, one of the first in vitro studies that analyzed phagocytic killing by neutrophils showed that a major mechanism by which CR-Kp has adapted to evade neutrophil-mediated killing is through evasion of phagocytosis ( 99 ). A later in vitro study using a distinct CR-Kp isolate demonstrated that phagocytosed CR-Kp can disrupt phagosome acidification and survive intracellularly for at least 2 h in murine neutrophils ( 100 ). Finally, a recent study showed that a single CR-Kp isolate impaired granule mobilization and inhibited NET release by neutrophils (a process called NETosis) ( 101 ); however, the mechanism underlying this phenomenon is still to be described.…”

Section: K Pneumoniae Interactions With Host Innate Immune Cellsmentioning

confidence: 99%

Finding Order in the Chaos: Outstanding Questions in Klebsiella pneumoniae Pathogenesis

et al. 2021

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Klebsiella pneumoniae are gram-negative facultative anaerobes that are found within host-associated commensal microbiomes, but they can also cause a wide range of infections that are often difficult to treat. These infections are caused by different pathotypes of K. pneumoniae, called either classical and hypervirulent strains. These two strain groups are genetically distinct, inhabit non-overlapping geographies, and cause different types of harmful infections in humans. These distinct bacterial groups have also been found to interact differently with the host immune system. Initial innate immune defenses against K. pneumoniae infection include complement, macrophages, neutrophils, and monocytes; these defenses are primary strategies employed by the host to clear infections. K. pneumoniae pathogenesis depends upon the interactions between the microbe and each of these host defenses, and it is becoming increasingly apparent that bacterial genetic diversity impacts the outcomes of these interactions. Herein we highlight recent advances in our understanding of K. pneumoniae pathogenesis, with a focus on how bacterial evolution and diversity impact K. pneumoniae interactions with mammalian innate immune host defenses. We also discuss outstanding questions regarding how K. pneumoniae can frustrate normal immune responses, capitalize upon states of immunocompromise, and cause infections with high mortality.

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“…In the United States, the infection caused by Carbapenem-resistant Enterobacteriaceae (CRE) produce up to 2.93 cases per 100,000 people ( Guh et al, 2015 ). CRE sequence type 258 (CRKP-ST258) is a multidrug-resistant strain that has spread worldwide, which evades the neutrophil immune response, preventing intracellular killing and NETs release in neutrophils derived from human ( Castillo et al, 2019 ) and mice ( Peñaloza et al, 2020 ). In human neutrophils, it was described that inhibition of NETs release was due to the avoidance of ROS production, produced to in part to the polysaccharide of the LPS ( Castillo et al, 2019 ).…”

Section: Role Of Neutrophil Extracellular Trap During Bacterial Infec...mentioning

confidence: 99%

“…In human neutrophils, it was described that inhibition of NETs release was due to the avoidance of ROS production, produced to in part to the polysaccharide of the LPS ( Castillo et al, 2019 ). In mouse neutrophils, no differences in ROS or MPO was observed when compared to a non-pathogenic K. pneumoniae , but differences in the acidification of the phagolysosome was described, which affects the functionality of MPO ( Peñaloza et al, 2020 ). Another in vitro study performed in human-derived neutrophils showed that K. pneumoniae carbapenem-resistant affects the release of NETs due to the mobilization of primary granules due to a non-soluble virulence factor ( Birnberg-Weiss et al, 2021 ).…”

Section: Role Of Neutrophil Extracellular Trap During Bacterial Infec...mentioning

confidence: 99%

Role of Extracellular Trap Release During Bacterial and Viral Infection

et al. 2022

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Neutrophils are innate immune cells that play an essential role during the clearance of pathogens that can release chromatin structures coated by several cytoplasmatic and granular antibacterial proteins, called neutrophil extracellular traps (NETs). These supra-molecular structures are produced to kill or immobilize several types of microorganisms, including bacteria and viruses. The contribution of the NET release process (or NETosis) to acute inflammation or the prevention of pathogen spreading depends on the specific microorganism involved in triggering this response. Furthermore, studies highlight the role of innate cells different from neutrophils in triggering the release of extracellular traps during bacterial infection. This review summarizes the contribution of NETs during bacterial and viral infections, explaining the molecular mechanisms involved in their formation and the relationship with different components of such pathogens.

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L-Arginine Enhances Intracellular Killing of Carbapenem-Resistant Klebsiella pneumoniae ST258 by Murine Neutrophils

Cited by 10 publications

References 56 publications

Two Phenotypes of Klebsiella pneumoniae ST147 Outbreak from Neonatal Sepsis with a Slight Increase in Virulence

Two Phenotypes of Klebsiella pneumoniae ST147 Outbreak from Neonatal Sepsis with a Slight Increase in Virulence

Finding Order in the Chaos: Outstanding Questions in Klebsiella pneumoniae Pathogenesis

Role of Extracellular Trap Release During Bacterial and Viral Infection

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