l-ascorbic acid provides a highly effective and environmentally sustainable method to control red imported fire ants

Ning, Dezhi; Hassan, Babar; Nie, Linru; Yang, Kui; Pan, Yuanqian; Pan, Zhongli; Xu, Yijuan

doi:10.1007/s10340-020-01203-z

Cited by 7 publications

(6 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Then, an open wound was made (e.g., in the abdomen or dorsal vessel of the insect). When a drop of haemolymph spilled out of any of the broken parts of the beetle, the individual was placed in the small centrifuge tube and centrifuged [ 18 ] ( Figure 2 C,F,I). The speed, time and temperature were consistent with those used for the net centrifugation method.…”

Section: Methodsmentioning

confidence: 99%

An Efficient and Simple Method for Collecting Haemolymph of Cerambycidae (Insecta: Coleoptera) Adults

Niu

Zhao

Shi

et al. 2022

Insects

View full text Add to dashboard Cite

Cerambycid beetles (Cerambycidae) are major forest pests, posing a serious threat to the security of forest resources worldwide. Extensive research has focused on the control of cerambycid beetles from physiological and biochemical perspectives. Despite the important roles of insect haemolymph in physiological processes, efficient collection methods for Cerambycidae are lacking. For the efficient and easy collection of large amounts of pure haemolymph from adult cerambycid beetles, a new method, named net centrifugation, was developed. Three species of cerambycid beetles with large differences in size, Anoplophora chinensis, Monochamus saltuarius and Saperda populnea, were selected for the study. Haemolymph was collected by the newly developed net centrifugation method—in which an inner nylon net is used during centrifugation under optimised conditions, and a relatively small wound is generated on the insect—as well as the traditional tearing method and double centrifugation method. Among the three methods evaluated, the net centrifugation method caused the least damage to cerambycid beetles during the whole operation. This method resulted in the most haemolymph from a single beetle, with the lowest turbidity, mostly pure haemocytes in the precipitate, the clearest haemolymph smears by microscopy and the highest quality of RNA extracted from haemocytes. The net centrifugation method has a high collection efficiency, providing important technical support for haemolymph extraction and entomological research.

show abstract

Section: Methodsmentioning

confidence: 99%

An Efficient and Simple Method for Collecting Haemolymph of Cerambycidae (Insecta: Coleoptera) Adults

Niu

Zhao

Shi

et al. 2022

Insects

View full text Add to dashboard Cite

show abstract

“…Chen and Oi (2020) indicated that the most promising alternative materials for prevention and controlling RIFA are natural compounds, of which only abamectin and spinosyn have been commercialized. Previous studies reported that baits containing ascorbic acid and autumn crocus stem powder have highly toxic effects on the RIFA (Lin et al, 2020; Ning et al, 2020). The sweeteners erythritol and saccharin also have lethal effects on the RIFA at relatively low concentrations (Zhang et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies reported that baits containing ascorbic acid and autumn crocus stem powder have highly toxic effects on the RIFA (Lin et al, 2020;Ning et al, 2020). The sweeteners erythritol and saccharin also have lethal effects on the RIFA at relatively low concentrations (Zhang et al, 2017).…”

mentioning

confidence: 99%

Lethal effect of ethanol on Solenopsis invicta Buren and economic fish

Song

Liang

Wang

et al. 2022

J Applied Entomology

Self Cite

View full text Add to dashboard Cite

The red imported fire ant (RIFA), Solenopsis invicta Buren, is an invasive pest species worldwide in many countries and regions. At present, its control mainly depends on synthetic insecticides. Although synthetic insecticides have the advantages of efficacy and convenience, their widespread use has a negative impact on the diversity of local arthropods. Ethanol is widely used as a disinfectant and has been consumed by people, but its insecticidal effect has not yet been evaluated. Here, the control effect of ethanol on the RIFA and its toxicity on four fish species were evaluated. The results exhibited that mortalities of all tested adult castes reached 100% after soaking in 70% v/v ethanol solution for one hour. All reproductive and worker pupae survived at 60% v/v ethanol treatment. In the soaking plus fumigation experiment, all adult castes and brood except female pupae were sensitive to 30% v/v ethanol solution. Conversely, female pupae showed the highest resistance to ethanol. More than 60% v/v ethanol concentration was needed to cause its 100% mortality. In the field experiment, 25% v/v and 50% v/v ethanol solution resulted in 70% and 80% reduction of mound 14 d after drench treatment, respectively. The ethanol vapour in the nest soil was higher than 80 ppm after 72 h of mound drench in the field with 25% v/v ethanol solution. Moreover, 30% v/v ethanol solution did not adversely affect the survival of four fish species exposed to the solution for a short time. This study suggests that mound drench can be alternatively employed with ethanol as a green organic solvent to control RIFA.

show abstract

“…However, in the case of Atta sexdens and Acromyrmex, only other acids were determined in the metapleural gland, with an emphasis on indole acetic acid and hydroxyhexanoic acid (Vander Meer, 2012). Recent studies have tested ascorbic acid, via diet, on the red imported fire ant, Solenopsis invicta Buren (Ning et al, 2020), and yielded promising results, given the significant laboratory mortality, with prospects for use in ant baits.…”

Section: Introductionmentioning

confidence: 99%

Mortality of leaf-cutting ants with salicylic acid

Fazam

Shimizu

Almeida

et al. 2021

Semina: Ciênc. Agrár.

View full text Add to dashboard Cite

Strategies for the control of leaf-cutting ants have mainly involved granular baits based on fipronil and sulfluramid as active ingredients, which are commonly coated with attractive citrus-based substances. Their constant use and the lack of alternatives in the market may favor the perception of these substances by ants, causing rejection for foraging and consequent difficulty in their control. In this respect, this study examined the mortality of leaf-cutting ants of the genera Atta and Acromyrmex subjected to direct application with dry powders, in laboratory conditions. As a preliminary treatment, a commercial antiseptic talc powder (C. A. P. T.) was used, followed by isolated treatments that corresponded to its components with potential insecticidal action, namely, salicylic acid, sulfur, boric acid, zinc oxide, in addition to an inert talc powder (Quimidrol®) as the control. For each treatment, 40 (worker) ants, whose activity was reduced due to remaining in a refrigerator prior to the treatment, were placed in transparent crystal polystyrene (‘Gerbox’ type) and were sprinkled with a salt shaker. The ants were kept at 25 ± 2 °C, under a 12-h photophase, and cumulative mortality was recorded every 24 h, considering dead ants as those that were unable to maintain the natural position of their body, i.e., even dying ants were considered dead when they exhibited no reaction when touched by a paintbrush. When sprinkled on ants, C. A. P. T. causes 40% mortality in both species 24 h after application. Subsequent studies of the components of this commercial product have found that when sprinkled on both Atta sexdens rubropilosa and Acromyrmex crassispinus, salicylic acid causes 100% mortality of workers in the first 24 h. This result provides a new prospect of control through a low environmental-impact product, representing an alternative for control in nests in the field that can contribute to the integrated control of ants.

show abstract

l-ascorbic acid provides a highly effective and environmentally sustainable method to control red imported fire ants

Cited by 7 publications

References 48 publications

An Efficient and Simple Method for Collecting Haemolymph of Cerambycidae (Insecta: Coleoptera) Adults

An Efficient and Simple Method for Collecting Haemolymph of Cerambycidae (Insecta: Coleoptera) Adults

Lethal effect of ethanol on Solenopsis invicta Buren and economic fish

Mortality of leaf-cutting ants with salicylic acid

Contact Info

Product

Resources

About