L-carnitine enhances developmental potential of bovine oocytes matured under high lipid concentrations in vitro

Catandi, G.; Cheng, Ming-Hao; Chicco, Adam J.; Carnevale, E.M.

doi:10.1016/j.anireprosci.2023.107249

Cited by 3 publications

(1 citation statement)

References 69 publications

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“…Increased NEFA levels in FF then alter the functions of granulosa cells and oocytes. In bovine, murine, and human cumulus-oocyte complexes matured in vitro, the negative effect of high NEFA concentrations (PA, STA, and OA) was observed in increased glucose uptake and lactate production, accumulation of lipids, reduced mitochondrial membrane potential, and oxidative stress [39,40]. These can reduce oocyte developmental competence [41].…”

Section: Can Supplementary Flaxseed Modify the Release Of Ovarian Hor...mentioning

confidence: 99%

Different Lengths of Diet Supplementation with 10% Flaxseed Alter the Hormonal Profile and the Follicular Fluid Fatty Acid Content of Fattening Gilts

Lecová,

Babjáková,

Sopková

et al. 2024

Life

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The effect of 10% dietary flaxseed fed for 3 and 6 weeks on serum hormone levels of fattening gilts, the fatty acid (FA) follicular fluid (FF) composition of small and large antral follicles, and the steroidogenesis and IGF-I secretion by isolated small antral follicles and their response to regulatory hormones (LH, FSH, IGF-I) was studied using immunoassay and gas chromatography analyses. Both supplemental periods increased levels of P4 and IGF-I in blood serum. A shorter period inhibited steroidogenesis (P4, T, E2) and IGF-I secretion by small antral follicles, which was associated with decreased levels of monounsaturated FAs (MUFA) and preferred n-6 polyunsaturated FA (PUFA) metabolism. A longer period stimulated hormone secretion at elevated levels of saturated FAs (SFA) at the expense of MUFAs and PUFAs preferring the n-3 PUFA metabolism. Out of ovarian regulators, only LH and IGF-I were able to alter the secretion of steroids and IGF-I by small follicles of fattening pigs fed a basal diet. The effect of flaxseed on the secretion of follicular hormones after both supplemental periods was altered by all regulatory hormones in a dose-dependent manner. The level of SFAs and PUFAs in FF of large follicles increased with the length of flaxseed feeding, suggesting the suppression of ovulation.

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Section: Can Supplementary Flaxseed Modify the Release Of Ovarian Hor...mentioning

confidence: 99%

Different Lengths of Diet Supplementation with 10% Flaxseed Alter the Hormonal Profile and the Follicular Fluid Fatty Acid Content of Fattening Gilts

Lecová,

Babjáková,

Sopková

et al. 2024

Life

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IMSIS: An instrumented microphysiological system with integrated sensors for monitoring cellular metabolic activities

Cheng,

Way,

Fresa

et al. 2024

Biosensors and Bioelectronics

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The efficacy of orally administered L-carnitine in alleviating ovarian dysfunctions has laid the foundation for targeted in vivo use: a study employing self-control and propensity score matching

Zhao,

Liu,

Zhang

et al. 2024

Front. Endocrinol.

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ObjectiveThis study aimed to evaluate the effectiveness of oral L-carnitine administration in patients after treatment failure to lay the groundwork for targeted in vivo use.Methods and materialsA total of 515 In Vitro Fertilization (IVF) patients undergoing subsequent cycles were included after applying exclusion criteria. They were divided into a control group of 362 patients and a study group of 153 patients who received oral L-carnitine until oocyte retrieval.140 patients were matched according to maternal age, infertility duration, body mass index (BMI), day three top-quality embryos rate, by propensity score matching (PSM). The study investigated the relationship between L-carnitine treatment and in vivo oocyte maturation, normal fertilization, and subsequent embryo development.ResultsFollowing PSM, initial differences in BMI and Day3 top-quality embryo rate between groups were nullified, we created two comparable cohorts with highly similar characteristics. In the subsequent cycles, the study group showed significant improvements in in vivo oocyte maturation rate at retrieval (p=0.002), normal in vitro fertilization rate (p=0.003), blastocyst formation rate (p=0.003), and usable blastocyst rate compared to controls. Although there was no significant difference in the top-quality embryo rate on Day 3, the study group showed a 10% increase in the upper quartile (55.35% vs. 66.67%). The cumulative clinical pregnancy and live birth rates showed a significant improvement (59.82% vs. 68.42%,p=0.004, 47.41% vs. 59.80%, p=0.002). Furthermore, self-control analysis revealed substantial enhancements (p<0.001) in all outcome measures following L-carnitine administration, resulting in the birth of 74 healthy neonates without congenital anomalies.ConclusionWe theorized that daily oral intake of L-carnitine before oocyte retrieval could boost oocyte quality and embryonic development, thus improving IVF outcomes. Ongoing investigations hold the potential to offer valuable insights into the applications and mechanisms underlying the therapeutic effectiveness of L-carnitine.

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L-carnitine enhances developmental potential of bovine oocytes matured under high lipid concentrations in vitro

Cited by 3 publications

References 69 publications

Different Lengths of Diet Supplementation with 10% Flaxseed Alter the Hormonal Profile and the Follicular Fluid Fatty Acid Content of Fattening Gilts

Different Lengths of Diet Supplementation with 10% Flaxseed Alter the Hormonal Profile and the Follicular Fluid Fatty Acid Content of Fattening Gilts

IMSIS: An instrumented microphysiological system with integrated sensors for monitoring cellular metabolic activities

The efficacy of orally administered L-carnitine in alleviating ovarian dysfunctions has laid the foundation for targeted in vivo use: a study employing self-control and propensity score matching

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