This study was conducted to consider the effect of theophylline in laying and some of egg characteristics on laying hens. Commercial egg type Hy-Line hens were used in a completely randomize design with 4 treatments and 3 replicates for 50 days period. The levels of different theophylline supplements (treatment) were 0 (control), one, three, and five mg per kg of body weight for 50 days. The average data were compared by using Tukey-Kramer multiple comparison tests. All levels of theophylline significantly increased laying performance. The best laying performance was observed in 3 mg/kg of theophylline. The best effect on egg size, weight, yolk and albumen height was found in hens receiving highest concentration of theophylline (p≤ 0.05). All levels of theophylline significantly decreased yolk color index (p≤ 0.05). The lowest yolk diameter was observed in 3 mg/kg theophylline. The highest albumen Haugh unit was in 1 mg/kg theophylline (p≤ 0.05). Results showed that theophylline increased the rate of laying and some of egg characteristics in hens. The best laying performance was showed in 3 mg/kg and the highest egg weight and size in 5 mg/kg of body weight theophylline.