l-Dopa synthesis using tyrosinase immobilized on magnetic beads

Co-Immobilization of Peroxidase and Tyrosinase onto Hybrid Membranes Obtained by the Sol-Gel Method for the Construction of an Optical Biosensor

“…On examining the temperature optimums and the influence of the matrix on the stability of the enzyme, the results indicated a more stable behaviour of the enzyme in a system containing cellulose acetate propionate with high molecular weight (CAP/H). The temperature profile obtained for the immobilized tyrosinase coincided with the one described by tunkagil et al (19).…”

“…the main organic elements in the hybrid materials are different natural and synthetic polymers such as cellulose derivatives (23), chitosan (2), gelatine (19), polymethylmetacrylate, polyvinyl alcohol, etc. (13).…”

Preparation and characterization of epoxy-functionalized magnetic chitosan beads: laccase immobilized for degradation of reactive dyes

“…In general, low stability and the potential for drastic reductions in enzymatic activity have been considered as hindrances to the practical application of enzymatic systems. It is well known that these cost-effective problems can be reduced, and its economic value can be increased by immobilization [11][12][13][14][15]. A variety of polymeric support materials including natural (chitin, chitosan, agarose, and cellulose derivatives) and synthetic (nylon, polysiloxane/polyaniline, polyvinylalcohol, poly(glycidyl methacrylate) and polyacrylic polymers) have been prepared in the various geometric form and used for the immobilization of enzyme [16][17][18][19][20].…”

Layer-by-Layer coated tyrosinase: An efficient and selective synthesis of catechols

“…Changes in pH-activity profile after immobilization, eventually involving the shift of the optimal pH value, have been reported during immobilization of tyrosinase on others support. 14,27 2.3. Storage stability…”

“…6 Moreover, the possibility to immobilize tyrosinase on stable and low cost supports further increase the interest for this enzyme in industrial applications. 7 Examples of the immobilization of tyrosinase on carbon nanotube, 8 copolymer matrices, 9 chitosan, 10 gold nanoparticles, 11 alumina sol-gel, 12 membrane alginate, polyacrylamide, and gelatine gels 13 have been reported in the production of L-dopa, 14 in the removal of phenolic compounds from waste water, 15 and in other industrial applications. 16 However, some of these immobilization methods are rather complicated and do not give good enzyme stability or retention.…”