L-Erythrulose production with a multideletion strain of Gluconobacter oxydans

Burger, Christian; Kessler, Constantin; Gruber, S.; Ehrenreich, Armin; Liebl, Wolfgang; Weuster‐Botz, Dirk

doi:10.1007/s00253-019-09824-w

Cited by 16 publications

(6 citation statements)

References 32 publications

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“…A possible limitation might be a higher number of membrane-bound dehydrogenases in G. oxydans compared with P. putida, which might limit the secretion of Fdh and invertase. A G. oxydans multideletion strain lacking eight membranebound dehydrogenases showed an increased Lerythrulose production by the native membrane-bound polyol dehydrogenase SldAB compared with the parental wild-type strain, which might be due at least in part to an improved Sec-dependent secretion of SldA (Peters et al, 2013;Burger et al, 2019). In summary, the results shown in Fig.…”

Section: Biotransformation With Resting Cells Of P Putida and G Oxydansmentioning

confidence: 62%

“…A G . oxydans multideletion strain lacking eight membrane‐bound dehydrogenases showed an increased l ‐erythrulose production by the native membrane‐bound polyol dehydrogenase SldAB compared with the parental wild‐type strain, which might be due at least in part to an improved Sec‐dependent secretion of SldA (Peters et al ., 2013 ; Burger et al ., 2019 ). In summary, the results shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

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Metabolic engineering of Pseudomonas putida for production of the natural sweetener 5‐ketofructose from fructose or sucrose by periplasmic oxidation with a heterologous fructose dehydrogenase

Wohlers

Wirtz

Reiter

et al. 2021

Microbial Biotechnology

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Summary5‐Ketofructose (5‐KF) is a promising low‐calorie natural sweetener with the potential to reduce health problems caused by excessive sugar consumption. It is formed by periplasmic oxidation of fructose by fructose dehydrogenase (Fdh) of Gluconobacter japonicus, a membrane‐bound three‐subunit enzyme containing FAD and three haemes c as prosthetic groups. This study aimed at establishing Pseudomonas putida KT2440 as a new cell factory for 5‐KF production, as this host offers a number of advantages compared with the established host Gluconobacter oxydans. Genomic expression of the fdhSCL genes from G. japonicus enabled synthesis of functional Fdh in P. putida and successful oxidation of fructose to 5‐KF. In a batch fermentation, 129 g l−1 5‐KF were formed from 150 g l−1 fructose within 23 h, corresponding to a space‐time yield of 5.6 g l−1 h−1. Besides fructose, also sucrose could be used as substrate for 5‐KF production by plasmid‐based expression of the invertase gene inv1417 from G. japonicus. In a bioreactor cultivation with pulsed sucrose feeding, 144 g 5‐KF were produced from 358 g sucrose within 48 h. These results demonstrate that P. putida is an attractive host for 5‐KF production.

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Section: Biotransformation With Resting Cells Of P Putida and G Oxydansmentioning

confidence: 62%

Section: Resultsmentioning

confidence: 99%

Metabolic engineering of Pseudomonas putida for production of the natural sweetener 5‐ketofructose from fructose or sucrose by periplasmic oxidation with a heterologous fructose dehydrogenase

Wohlers

Wirtz

Reiter

et al. 2021

Microbial Biotechnology

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“…In addition, Peters et al ( 2013 ) used G. oxydans BP.9 to express genes encoding mDHs from the metagenome of a mother of vinegar sample including many non-culturable AAB and other microorganisms. In 2019, Burger et al applied G. oxydans BP.8 to investigate and optimize the production of l -erythrulose (Burger et al, 2019 ).…”

Section: Molecular Biological Methods For Aofmentioning

confidence: 99%

Oxidative Fermentation of Acetic Acid Bacteria and Its Products

Xie

Zhang

et al. 2022

Front. Microbiol.

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Acetic acid bacteria (AAB) are a group of Gram-negative, strictly aerobic bacteria, including 19 reported genera until 2021, which are widely found on the surface of flowers and fruits, or in traditionally fermented products. Many AAB strains have the great abilities to incompletely oxidize a large variety of carbohydrates, alcohols and related compounds to the corresponding products mainly including acetic acid, gluconic acid, gulonic acid, galactonic acid, sorbose, dihydroxyacetone and miglitol via the membrane-binding dehydrogenases, which is termed as AAB oxidative fermentation (AOF). Up to now, at least 86 AOF products have been reported in the literatures, but no any monograph or review of them has been published. In this review, at first, we briefly introduce the classification progress of AAB due to the rapid changes of AAB classification in recent years, then systematically describe the enzymes involved in AOF and classify the AOF products. Finally, we summarize the application of molecular biology technologies in AOF researches.

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“…For G. oxydans , studies were mainly performed to identify the function of some genes by gene knockout, such as the gene cluster for the biosynthesis of PQQ [ 18 ] and the characterization of membrane-bound dehydrogenases from G. oxydans 621H [ 19 ]. Knockout of eight membrane-bound dehydrogenases in G. oxydans 621H could improve the titer and conversion rate of l -erythrulose to 242 g/L and 99%, respectively, in a dissolved oxygen (DO)-controlled stirred-tank bioreactor [ 20 ]. Thus, deleting enzymes unrelated to the l -sorbose formation may enhance the l -sorbose production by avoiding unnecessary energy consumption and providing limited membrane space to the most needed membrane-bound D-sorbitol dehydrogenase [ 21 ].…”

Section: Introductionmentioning

confidence: 99%