l'INSÉMINATION Artificielle De La Truite (Salmo Gairdneri Richardson). Iii. — Définition De La Nature Et De La Molarité Du Tampon a Employer Avec Les Dilueurs d'INSÉMINATION Et De Conservation

Billard, R.; Jalabert, Bernard; Breton, Bernard; Escaffre, Anne-Marie; Carpentier, M.

doi:10.1051/rnd:19740404

Cited by 10 publications

(2 citation statements)

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“…Kusa (1950) reported that Oncorhynchus keta spermatozoa lost their fertilizing ability in Ringer’s solution after 90 min; however, the latter can be regained after 24 h. After dilution of Salmo trutta sperm in Ringer’s solution, Ginsburg (1972) noted a loss of fertility 20 min later, but a complete recovery was observed after 90 and 120 min. The same pattern was also found for spermatozoa treated with ovarian fluid although sperm ability to fertilize was at a lower level (Billard et al., 1974). When inseminating eggs at consecutive intervals, dilution of Oncorhynchus keta milt in Ringer’s medium (followed by a second dilution in the same medium) resulted in a gradual decrease in fertilizing ability down to zero at 120 min (Yamamoto, 1961).…”

Section: Introductionmentioning

confidence: 99%

Comparison of sperm velocity, motility and fertilizing ability between firstly and secondly activated spermatozoa of common carp (Cyprinus carpio)

Linhart¹,

Alavi²,

Rodina³

et al. 2008

Journal of Applied Ichthyology

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The objective of the study was to compare carp sperm motility performances (sperm velocity and motility rates) from 10 males including fertilizing ability (hatching rates from 10 males and eight females) as a function of time elapsed after sperm exposure to activation medium in two situations: firstly activated sperm and sperm which had terminated swimming and was Ôre-activatedÕ after incubation in a K + rich (200 mM M KCl) non-swimming solution. In case of both initial (first) and secondly activated spermatozoa, the motility was triggered in hatchery solution (HAS, 11.2 mOsmol) and in carp activation solution (CAS, 128.9 mOsmol) containing 45 mM M NaCl, 5 mM M KCl, 30 mM M Tris-HCl while also adjusted to a pH of 8.0. First time activated sperm showed significantly higher relative motility, sperm velocity and fertilizing ability compared to re-activated sperm. The carp spermatozoa (in either first or second activation) rapidly lost their fertilizing ability as a function of exposure time of sperm to diluents prior to addition to eggs: this shows that spermatozoa must be in contact with eggs as soon as their motility is triggered. When sperm was firstly activated in CAS and also activated a second time in CAS (labeled CASCAS) the hatching rate was significantly higher at egg contact after 10, 20, 30, and 120 s of activation. Also at 20 s after the second activation of the sperm higher sperm motility was observed compared to the first activation. This study showed that incubation of spermatozoa in a K + -rich incubation medium can mitigate the affects of structural damages occurring in re-activated sperm, which may help spermatozoa to increase their motility and fertilization. To our knowledge, the results presented in this study document for the first time that fertilization can be achieved with sperm re-activated a second time while being exposed to a incubation medium that permits ATP reloading within the flagellum. Previous studies have show the potential for recovery of motility, however, the effect on possible fertilization is hitherto unknown. It critical outcome of the study clearly indicated the need for avoiding the use of different, subsequent activation media (e.g. first and second activation) but only on the same medium for both steps (see above CASCAS).

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Section: Introductionmentioning

confidence: 99%

Comparison of sperm velocity, motility and fertilizing ability between firstly and secondly activated spermatozoa of common carp (Cyprinus carpio)

Linhart¹,

Alavi²,

Rodina³

et al. 2008

Journal of Applied Ichthyology

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“…Le matériel animal et les conditions expérimentales ont été définies dans les notes précédentes BILLARD, JALABERT, BRETON, 1974). Les expériences spécifiquement conduites dans cette étude sont les suivantes :…”

Section: Materiel Et Methodesunclassified

L'insémination artificielle de la truite Salmo gairdneri Richardson. V. - Effets de la dilution et définition du rapport optimum gamètes/dilueur.

Billard

1975

Bull. Fr. Piscic.

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Le pourcentage de fécondation des ovules de Truite arc-en-ciel est diminué lorsque le volume de dilueur employé lors de l'insémination artificielle est augmenté (Fig. 1). Les expériences sur la conservation de la fertilité des gamètes après dilution montrent que ce sont surtout les spermatozoïdes qui sont affectés par la dilution (Fig. 4). Par contre la dilution de grandes quantités de sperme dans des volumes réduits de dilueurs tend à diminuer le pourcentage de fécondation ce qui revient à mettre en cause la méthode sèche pratiquée en pisciculture. A volume constant de dilueur (20 ml) et avec des quantités variables de gamètes (100 à 1000 ovules et 0,2 µxl à 2 ml de sperme) le pourcentage maximum de fécondation est obtenu lorsque le nombre d'ovules ne dépasse pas 200 ou 600 pour des taux de dilution respectivement de 10-4 à 10-3. Dans les cas les plus favorables, c'est-à-dire lorsque tous les ovules fécondables sont fécondés à une dilution de 10-3, le nombre minimum de spermatozoïdes par oeuf est de l'ordre de 10.000. Quant au nombre optimum de spermatozoïdes par oeuf à utiliser lors de l'insémination, il est de l'ordre de 300.000 (avec un taux de dilution de10-3). En conclusion, il existe un rapport optimum entre le nombre d'ovules, la quantité de sperme et le volume de dilueur à mettre en oeuvre lors de l'insémination pour en obtenir l'efficacité maximum ; nombre d'ovules par ml de dilueur compris entre 10 et 20 pour un taux de dilution final du sperme dans le dilueur de 10-3.
The percentage of fertilization of rainbow trout ovules is reduced when the volume of diluent added when inseminating is increased. Experiments conducted to check the effects of dilution on gamète survival before insémination showed that spermatozoa are more affected than ovules by the dilution. Conversely large amount of sperm diluted in small volumes of diluent reduces the rate of fertilization ; then the validity of the dry method for artificial insémination is questioned. At constant volume of diluent (20 ml) and various amount of gamètes (100 to 1.000 ovules and 0,2 µxl to 2ml sperm) high percentage of fertilization was obtained when egg number was lesser than 200 and 600 at dilution rate of 10-4 and 10-3 respectively. In the best conditions, when most of the ovules are fertilized at a dilution rate of 10-3, the minimum number of spermatozoa per egg is about 10 000. But the optimum number of spermatozoa/egg to be used for insémination is 300.000 (dilution rate = 10-3). In conclusion, there is an optimum ratio to observe between number of ovules, quantity of sperm and amount of diluent when inseminating in order to obtain maximum efficiency ; number of ovules per ml diluent falls between 10 and 20 for a final dilution rate of sperm in the diluent of 10-3

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Rapid and quantitative assessment of trout spermatozoa motility using stroboscopy

et al. 1985

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HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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l'INSÉMINATION Artificielle De La Truite (Salmo Gairdneri Richardson). Iii. — Définition De La Nature Et De La Molarité Du Tampon a Employer Avec Les Dilueurs d'INSÉMINATION Et De Conservation

Cited by 10 publications

References 0 publications

Comparison of sperm velocity, motility and fertilizing ability between firstly and secondly activated spermatozoa of common carp (Cyprinus carpio)

Comparison of sperm velocity, motility and fertilizing ability between firstly and secondly activated spermatozoa of common carp (Cyprinus carpio)

L'insémination artificielle de la truite Salmo gairdneri Richardson. V. - Effets de la dilution et définition du rapport optimum gamètes/dilueur.

Rapid and quantitative assessment of trout spermatozoa motility using stroboscopy

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