l(+)-lactic acid production by repeated batch culture of Rhizopus oryzae in air-lift bioreactor

Yin, Ping; Yahiro, Kazutoyo; Ishigaki, Tooru; Park, Yong Soo; Okabe, Mitsuyasu

doi:10.1016/s0922-338x(97)80361-3

Cited by 67 publications

(32 citation statements)

References 13 publications

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“…The Wnal LLAC of the Wrst cycle reached 103.7 g/l, and volumetric productivity was 2.16 g/(l·h). In the following 19 cycles of repeated fermentation, the Wnal LLAC reached 81-95 g/l and volumetric productivities were 3.40-3.85 g/(l·h), those values being higher than those reported by Yin et al [8] and Bai et al [5]. In the last Wve cycles (cycles 21-25), because of pellet rupture and fungi autolysis, the metabolic capability of R. oryzae decreased, leading to decrease of volumetric productivity and LLAC, from 3.37 to 2.91 g/(l·h) and from 80 to 71 g/l, respectively.…”

Section: L-lactic Acid Production Using Magnetically Stirred Fermentocontrasting

confidence: 58%

“…Yu et al used Xocform R. oryzae to produce L-lactic acid by semicontinuous fermentation; for the Wrst four cycles the volumetric productivity and yield were 4.03 g/(l·h) and 0.90, respectively, in 28 h, but volumetric productivity decreased to 3.44 g/ (l·h) in 32 h in cycle 5 and cycle 6 [7]. Yin et al used small mycelia pellets of R. oryzae to produce L-lactic acid for nine cycles over 14 days, and the average volumetric productivity was 2.02 g/(l·h) for the Wrst six cycles in an air-lift bioreactor [8]. Du et al used two diVerent physical forms (Wlamentous and pellet) to produce L-lactic acid by R. oryzae in semicontinuous fermentation; the volumetric productivities were 5.06 and 4.39 g/(l·h), respectively, in the second cycle, but decreased to 2.36 and 2.93 g/(l·h) in the third cycle [9].…”

Section: Introductionmentioning

confidence: 99%

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Production of l-lactic acid by Rhizopus oryzae using semicontinuous fermentation in bioreactor

Jiang

Liu

et al. 2010

J Ind Microbiol Biotechnol

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Semicontinuous fermentation using pellets of Rhizopus oryzae has been recognized as a promising technology for L-lactic acid production. In this work, semicontinuous fermentation of R. oryzae AS 3.819 for L-lactic acid production has been developed with high L-lactic acid yield and volumetric productivity. The effects of factors such as inoculations, CaCO₃ addition time, and temperature on L-lactic acid yield and R. oryzae morphology were researched in detail. The results showed that optimal fermentation conditions for the first cycle were: inoculation with 4% spore suspension, CaCO₃ added to the culture medium at the beginning of culture, and culture temperature of 32-34 °C. In orthogonal experiments, high L-lactic acid yield was achieved when the feeding medium was (g/l): glucose, 100; (NH₄)₂SO₄, 2; KH₂PO₄, 0.1; ZnSO₄·7H₂O, 0.33; MgSO₄·7H₂O, 0.15; CaCO₃, 50. Twenty cycles of semicontinuous fermentation were carried out in flask culture. L: -lactic acid yield was 78.75% for the first cycle and 80-90% for the repeated cycles; the activities of lactate dehydrogenases (LDH) were 7.2-9.2 U/mg; fermentation was completed in 24 h for each repeated cycle. In a 7-l magnetically stirred fermentor, semicontinuous fermentation lasted for 25 cycles using pellets of R. oryzae AS 3.819 under the optimal conditions determined from flask cultures. The final L-lactic acid concentration (LLAC) reached 103.7 g/l, and the volumetric productivity was 2.16 g/(l·h) for the first cycle; in the following 19 repeated cycles, the final LLAC reached 81-95 g/l, and the volumetric productivities were 3.40-3.85 g/(l·h).

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Section: L-lactic Acid Production Using Magnetically Stirred Fermentocontrasting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Production of l-lactic acid by Rhizopus oryzae using semicontinuous fermentation in bioreactor

Jiang

Liu

et al. 2010

J Ind Microbiol Biotechnol

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“…Rhizopus species, such as R. oryzae and R. arrhizus, have amylolytic enzymatic activity, which enables them to convert starch directly to L(+)-lactic acid. Several studies have reported on the use of Rhizopus for lactic acid production (Yin et al 1998;Liu et al 2006;Yu et al 2007;Guo et al 2010;Wu et al 2011;Saito et al 2012). Fig.…”

Section: Bioresourcescommentioning

confidence: 99%

Lactic Acid Production to Purification: A Review

Komesu

Oliveira

Martins

et al. 2017

BioRes

209

114

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Lactic acid is a naturally occurring organic acid that can be used in a wide variety of industries, such as the cosmetic, pharmaceutical, chemical, food, and, most recently, the medical industries. It can be made by the fermentation of sugars obtained from renewable resources, which means that it is an eco-friendly product that has attracted a lot of attention in recent years. In 2010, the U.S. Department of Energy issued a report that listed lactic acid as a potential building block for the future. Bearing the importance of lactic acid in mind, this review summarizes information about lactic acid properties and applications, as well as its production and purification processes.

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“…These studies have taken two basic approaches, immobilization of cells (Hang et al, 1989;Tamada et al, 1992;Hamamci and Ryu, 1994;Dong et al, 1996;Xuemei et al, 1999), and promotion of mycelial or pellet morphology (Yang et al, 1995;Kosakai et al, 1997;Du et al, 1998;Park et al, 1998;Yin et al, 1998;Zhou et al, 2000). The term pellet morphology can be the source of some confusion in the discussion of optimal fungal morphology, since pellets of less than about one millimeter are associated with high production rates and yields, whereas larger pellets are not.…”

Section: L-lactic Acidmentioning

confidence: 99%