L-Methionine enhances the contractile response to norepinephrine of rat vas deferens.

Ichida, Seiji; Yoshioka, Kazuo; Oka, Hiroaki; Oda, Yasuo; Ariyoshi, Shunji

doi:10.1254/jjp.44.361

Cited by 3 publications

(4 citation statements)

References 6 publications

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“…Measurement of contractile responses: Segments of rat ileum, stomach fundus and uterus and guinea pig taenia coli were pre pared as described previously (9)(10)(11). Briefly, the ileum, stomach fundus and uterus of rats and taenia coli of guinea pigs were isolated, and cut into several segments (about 1.5x12 mm).…”

Section: Methodsmentioning

confidence: 99%

“…These muscle segments were equilibrated with each solution for a minimum of 30 min, and then their contractile responses were examined. The contractile responses of segments of the various muscles used were measured iso tonically with a force-displacement transducer (TD-1125, Nihon Kohden Kogyo Co., Tokyo, Japan) and recorder (Type 3056-11, Nihon Kohden Kogyo Co.), and the isotonic muscle tensions of those of rat ileum, stomach fundus and uterus and guinea pig taenia coli were adjusted to about 0.2, 1, 0.1 and 1 g, respec tively, as reported previously (10,11).…”

Section: Methodsmentioning

confidence: 99%

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Effects of Synthetic ω-Conotoxin on the Contractile Responses of Segments of Rat Ileum, Stomach Fundus and Uterus and Guinea Pig Taenia Coli

Ichida

Oka

Masada

et al. 1988

Japanese Journal of Pharmacology

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Abstract-The effect of synthetic w-conotoxin (w-CgTX) on the contractile re sponses of segments of rat ileum, stomach fundus and uterus and guinea pig taenia coli were investigated. w-CgTX (10-9-5 X 10-6 M) did not inhibit the contractile responses of al! smooth muscle segments to high KCI and/or ACh. However, unexpectedly, w-CgTX (3x10-7-10-5 M) alone caused dose-dependent con traction of segments of the stomach fundus and uterus. These contractile responses to w-CgTX alone depended upon the presence and/or the influx of extracellular Cat+; and they were inhibited by calcium antagonists such as diltiazem, nitrendipine and verapamil, with the exception that the segments of stomach fundus was not inhibited by verapamil. With the segments of uterus, but not those of other tissues, w-CgTX (10-7-5X10-6 M) significantly enhanced the contractile responses to various concentrations of ACh and high KCI. With rat ileum and guinea pig taenia coli segments, w-CgTX (10-9-5x10-6 M) did not induce a contractile response or have an enhancing effect. These findings suggest that w-CgTX may have a calcium agonist-like effect on smooth muscles such as the stomach fundus and uterus of rats.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Effects of Synthetic ω-Conotoxin on the Contractile Responses of Segments of Rat Ileum, Stomach Fundus and Uterus and Guinea Pig Taenia Coli

Ichida

Oka

Masada

et al. 1988

Japanese Journal of Pharmacology

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“…Contractile re sponses of rat uteri to KC1 and acetylcholine are significantly increased by methionine [ 10], effects which take 15 min of preincuba tion with methionine to be maximal, and have an EC50 for potentiation of 0.56 mmol/I. Similar effects have been found with rat vasa deferentia [11], These effects of methionine are thought to result from increased forma tion of S-adenosyl-T-methionine from methi onine, resulting in methylation of cell mem brane phospholipids. This methylation of the lipids increases membrane fluidity and is thought to affect physical parameters of the membrane, for example causing enhanced coupling of membrane receptors with adenyl ate cyclase [12], However, higher concentra tions of methionine had no effects on the con tractile response of rabbit whole bladders to nerve stimulation, suggesting that méthyl ation of S-adenosyl-L-methioninc may be less important in the bladder than in other smooth muscles.…”

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confidence: 54%

Influence of the Bathing Medium on the Contractile Responses of the Rabbit Urinary Bladder

Samadzadeh

Tong²,

Wein

et al. 1992

Pharmacology

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This study examined contractile responses of the in vitro rabbit whole-bladder preparation to field stimulation, bethanechol and KCl in Tyrode’s solution and minimum essential medium (MEM). We found frequency-dependent increases in intravesical pressure in bladders incubated in Tyrode’s solution and MEM. However, bladders incubated in MEM consistently responded with greater increases in intravesical pressure compared to those incubated in Tyrode’s solution. Similarly, there were frequency-dependent increases in the rate of pressure generation in bladders incubated in Tyrode’s solution and MEM, and bladders incubated in MEM responded with greater rates of pressure generation than those incubated in Tyrode’s solution at frequencies of 1 2 and 4 Hz. In addition, there were significant increases in intravesical pressure generated in response to administration of 500 mmol/l bethanechol and 186.4 mmol/l KCl in bladders incubated in MEM compared to Tyrode’s solution but no changes in the rate of pressure generation. The influence of L-methionine, one of the constituents of MEM, on the responses of whole bladders to nerve stimulation was also investigated. L-methionine at concentrations of 0.1 and 1.0 mmol/l had no effects on the increase in intravesical pressure or rate of pressure generation following nerve stimulation. It is speculated that the increases in contractile responsiveness of bladders incubated in MEM are related to the combination of amino acids, vitamins and other constituents of the cell culture medium.

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“…Since administration of Met enhances the contractile response to NE of rat blood vessel and exhibit stronger contraction in the presence of NE than those of normal rats, 29) we next examined whether in vivo long-term co-administration of GS with Met affects NE-induced aortic ring contraction in H-Hcy rats. For this, we prepared endothelium-denuded aortic rings from normal control, GS alone, Met, and GSϩMet for 60 d and examined NE-induced dose-response contractions in the range of 0.1 nM to 10 mM.…”

Section: Effects Of Gs On Vasomotor Activity In H-hcy Ratsmentioning

confidence: 99%

Ginseng Saponins Diminish Adverse Vascular Effects Associated with Chronic Methionine-Induced Hyperhomocysteinemia

Kim

Cho

Kang

et al. 2006

Biological & Pharmaceutical Bulletin

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Recent studies have shown that Panax ginseng has a variety of beneficial effects on the cardiovascular systems. Homocysteine (Hcy), which is derived from L-methionine (Met), has been closely associated with the increased risk of cardiovascular diseases. In the present study, we examined whether in vivo long-term administration of ginseng saponins (GS), active ingredients of Panax ginseng, attenuate adverse vascular effects associated with chronic Met-induced hyperhomocysteinemia (H-Hcy). We found that plasma Hcy level, which was measured after 30 and 60 d, in GS (100 mg/kg)؉Met co-administration group was significantly reduced when it was compared with Met alone treatment group. We could also observe the alleviation of endothelial damages of aortic artery vessels in GS (100 mg/kg)؉Met co-administration group compared with Met alone treatment group. We compared aortic vasocontractile and vasodilatory responses between Met alone and GS (100 mg/ kg)؉Met co-treatment groups. We found that norepinephrine-induced vasocontractile responses were greatly decreased in GS (100 mg/kg)؉Met co-treatment group and that carbachol-induced dilatory responses were greatly enhanced in GS (100 mg/kg)؉Met co-administration groups as compared with Met alone treatment group. The present results indicate that in vivo long-term administration of GS attenuates adverse vascular effects associated with chronic Met-induced H-Hcy in rats.

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L-Methionine enhances the contractile response to norepinephrine of rat vas deferens.

Cited by 3 publications

References 6 publications

Effects of Synthetic ω-Conotoxin on the Contractile Responses of Segments of Rat Ileum, Stomach Fundus and Uterus and Guinea Pig Taenia Coli

Effects of Synthetic ω-Conotoxin on the Contractile Responses of Segments of Rat Ileum, Stomach Fundus and Uterus and Guinea Pig Taenia Coli

Influence of the Bathing Medium on the Contractile Responses of the Rabbit Urinary Bladder

Ginseng Saponins Diminish Adverse Vascular Effects Associated with Chronic Methionine-Induced Hyperhomocysteinemia

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