L-Nω-Nitroarginine Inhibits the Induction of Nitric Oxide Synthase (iNOS) and Cyclooxygenase-2 (COX-2) by Inhibiting NF-κB and AP-1 Activation in RAW 264.7 Cells

Karna, Shibendra Kumar Lal; Ahamad, Faiz

doi:10.4172/2169-0138.1000135

Cited by 2 publications

(2 citation statements)

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“…20 Gene expression level assay MDA-MB-231 cell line was plated at a density of 1 × 10 6 cells/well in 6-well plates. 21 The genes for IL-6, IL-1β, TNF-α, Cyclin D1, BCL-2, and caspase-3 After 24 hours of incubation with the selected concentrations of AgNPs, fold changes were calculated. 22 Ribonucleic Acid (RNA) Extraction and Analysis RNeasy Mini kit was used to isolate total RNA (QIAGEN, USA).…”

Section: Cytotoxicity Assaymentioning

confidence: 99%

The Toxic Impact of Silver Nanoparticles Synthesized Extracellularly by Aspergillus flavus on Breast Cancer Cells (MDA-MB-231), as Well as Their Antibacterial Activity

2023

TJNPR

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The inherent antibacterial, antiviral, and anticancer characteristics of multifaceted nanoparticles have led to their application in various biological contexts. 3 The biosynthesis of AgNPs utilizing biomolecules such as enzymes/proteins, amino acids, carbohydrates, and vitamins has emerged as a simple, effective, economical, and environmentally friendly approach. This method leverages the properties of bacteria, fungi, as well as plant extracts, and small biomolecules like amino acids and vitamins. 2,4,5 Interestingly, certain AgNPs have exhibited exceptional efficacy against diverse bacteria species that have developed high levels of resistance to conventional pharmaceuticals. 6,7 Furthermore, nanoparticles have demonstrated their potential as carriers for delivering antimicrobial medications to infected organs within the body, capitalizing on their incredibly small size. 8,9 It is plausible that these biogenic nanoparticles may serve as viable alternatives to existing medications, which bacteria have evolved resistance against. In addition to their biomedical applications, these non-toxic nanoparticles offer a promising solution to address the issue of microbial contamination in drinking water. 10 Every year, millions of individuals, particularly in developing nations, suffer from waterborne diseases caused by microbial contaminants. In this regard, AgNPs have shown remarkable potential as an effective water disinfectant, enabling the provision of clean and safe drinking water, free from harmful microbes, and suitable for human consumption. By harnessing the disinfecting capabilities of these nanoparticles, we have the opportunity to make a significant impact on global public health. 2,10,11

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Section: Cytotoxicity Assaymentioning

confidence: 99%

The Toxic Impact of Silver Nanoparticles Synthesized Extracellularly by Aspergillus flavus on Breast Cancer Cells (MDA-MB-231), as Well as Their Antibacterial Activity

2023

TJNPR

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“…However, although the mechanism of silver nanoparticle synthesis by fungi is not fully understood, the fungi showed their ability to produce a greater amount of reducing agents compared to bacteria, and thus their ability to produce a greater amount of silver nanoparticles. It was reported that fungi synthesize nanoparticles by wrapping around the surface of Ag + ions and then reducing silver ions enzymatically by the enzymes of Naphthoquinones, Anthraquinones and nitrate reducing enzymes (Jha et al, 2009;(Klaus et al, 2009;Karna et al, 2016;Krishnan et al, 2020). But what differentiates the use of microorganisms (bacteria and fungi) in the synthesis of nanoparticles is that it is a slow process and it represents the main disadvantages when compared to the use of plant extracts.…”

Section: Biological Synthesis Of Silver Nanoparticlesmentioning

confidence: 99%

Silver Nanoparticles' Therapeutic Antibacterial, Antiproliferative, and Toxicological Effects.

Al-alwani

Almashaleh

2022

J. basic appl. Res. Biomed.

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Silver nanoparticles, among others, have broad-spectrum antimicrobial properties. Silver nanoparticles have suppressed dangerous microorganisms in medical and agricultural settings in several studies. Chemicals are harmful to humans and the environment, raising awareness of bioactive synthetic methods. These methods produce nanoparticles with better physicochemical qualities, stability, and toxicity. Biogenic nanoparticles can be made from bacterial and fungal byproducts that reduce and stabilize. Encapsulating these nanoparticles with biomolecules from the producing organisms may boost stability and biological activity. Nanoparticles' quick, clean, cheap, and ecological biologic manufacturing technique increases biocompatibility. Silver nanoparticles affect fish, algae, cell-based in vitro procedures, and microbes. Even though most of these studies were done quickly in well-regulated labs with much higher silver ion concentrations than in real life. Many silver types undergo long-term chemical transformation at extremely low levels (ng/L to g/L) in aquatic ecosystems. Thus, silver nanoparticles' environmental and health hazards need additional investigation. Recently detected antimicrobial silver at 10102 μg/mL. Multiple processes make silver nanoparticles dangerous. Basic (Ag0) and monovalent (Ag+) silver are most poisonous. Silver framework free ions affect silver toxicity. ROS damage DNA when elemental or zero-valent silver penetrates tissues. Packaged foods, contaminated water, swimming pools, antifouling, nasal and throat medicines, and other pharmaceuticals include silver nanoparticles. Consumption accumulates silver ions in subcutaneous fat. Prolonged exposure causes argyria-blue-gray skin. Silver inhibits Na+ and Cl absorption, disrupting electrolytes. Airborne silver nanoparticles may influence chronic pulmonary disease patients. Silver ions oxidize enzyme thiols, hindering electron transport and DNA replication. Ag+ rapidly damages DNA and RNA. Silver nanoparticle breakdown into silver ions creates germ-killing ROS. Silver nanoparticles are more hazardous than silver ions in the same atmosphere.

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L-Nω-Nitroarginine Inhibits the Induction of Nitric Oxide Synthase (iNOS) and Cyclooxygenase-2 (COX-2) by Inhibiting NF-κB and AP-1 Activation in RAW 264.7 Cells

Cited by 2 publications

References 0 publications

The Toxic Impact of Silver Nanoparticles Synthesized Extracellularly by Aspergillus flavus on Breast Cancer Cells (MDA-MB-231), as Well as Their Antibacterial Activity

The Toxic Impact of Silver Nanoparticles Synthesized Extracellularly by Aspergillus flavus on Breast Cancer Cells (MDA-MB-231), as Well as Their Antibacterial Activity

Silver Nanoparticles' Therapeutic Antibacterial, Antiproliferative, and Toxicological Effects.

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