L-Proline Activates Mammalian Target of Rapamycin Complex 1 and Modulates Redox Environment in Porcine Trophectoderm Cells

Liu, Ning; Yang, Ying; Si, Xuemeng; Hai, Jian; Zhang, Yunchang; Jiang, Da; Dai, Zhaolai; Wu, Zhenlong

doi:10.3390/biom11050742

Cited by 9 publications

(9 citation statements)

References 61 publications

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“…As expected, specific signaling modulators such as halofuginone (AAR inducer), SB431542 (TGFβ inhibitor), CHIR99021 (WNT agonist) and PD0325901 (MEK/ERK inhibitor) fully counteract L-Pro supplementation effects (Comes et al, 2013;D'Aniello et al, 2015). Moreover, L-Pro impacts mTOR pathway in porcine trophectoderm cells (Liu et al, 2021).…”

Section: Proline Modulates Signaling Pathwayssupporting

confidence: 56%

The Multifaceted Roles of Proline in Cell Behavior

Patriarca

Cermola

D’Aniello

et al. 2021

Front. Cell Dev. Biol.

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Herein, we review the multifaceted roles of proline in cell biology. This peculiar cyclic imino acid is: (i) A main precursor of extracellular collagens (the most abundant human proteins), antimicrobial peptides (involved in innate immunity), salivary proteins (astringency, teeth health) and cornifins (skin permeability); (ii) an energy source for pathogenic bacteria, protozoan parasites, and metastatic cancer cells, which engage in extracellular-protein degradation to invade their host; (iii) an antistress molecule (an osmolyte and chemical chaperone) helpful against various potential harms (UV radiation, drought/salinity, heavy metals, reactive oxygen species); (iv) a neural metabotoxin associated with schizophrenia; (v) a modulator of cell signaling pathways such as the amino acid stress response and extracellular signal-related kinase pathway; (vi) an epigenetic modifier able to promote DNA and histone hypermethylation; (vii) an inducer of proliferation of stem and tumor cells; and (viii) a modulator of cell morphology and migration/invasiveness. We highlight how proline metabolism impacts beneficial tissue regeneration, but also contributes to the progression of devastating pathologies such as fibrosis and metastatic cancer.

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Section: Proline Modulates Signaling Pathwayssupporting

confidence: 56%

The Multifaceted Roles of Proline in Cell Behavior

Patriarca

Cermola

D’Aniello

et al. 2021

Front. Cell Dev. Biol.

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“…Since SLC36A2 was found highly enriched in uEVs of pregnant mares and showed higher expression in the endometrium compared to the embryo, SLC36A2 mRNA and protein are probably secreted by the endometrium and transferred to the embryo. Previous in vitro studies with porcine trophoblast cells and mouse embryos 71 , 72 revealed an important role in providing proline which is essential for early embryonic development due to its role in the synthesis of polyamines 73 .…”

Section: Discussionmentioning

confidence: 99%

Uterine extracellular vesicles as multi-signal messengers during maternal recognition of pregnancy in the mare

Vegas

Hamdi

Podico

et al. 2022

Sci Rep

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In contrast to other domestic mammals, the embryo-derived signal(s) leading to maternal recognition of pregnancy (MRP) are still unknow in the mare. We hypothesize that these embryonic signals could be packed into uterine extracellular vesicles (uEVs), acting as multi-signal messengers between the conceptus and the maternal tract, and contributing to MRP. To unveil these signals, the RNA and protein cargos of uEVs isolated from uterine lavages collected from pregnant mares (P; day 10, 11, 12 and 13 after ovulation) and cyclic control mares (C; day 10 and 13 after ovulation) were analyzed. Our results showed a fine-tuned regulation of the uEV cargo (RNAs and proteins), by the day of pregnancy, the estrous cycle, and even the size of the embryo. A particular RNA pattern was identified with specific increase on P12 related to immune system and hormonal response. Besides, a set of proteins as well as RNAs was highly enriched in EVs on P12 and P13. Differential abundance of miRNAs was also identified in P13-derived uEVs. Their target genes were linked to down- or upregulated genes in the embryo and the endometrium, exposing their potential origin. Our study identified for first time specific molecules packed in uEVs, which were previously associated to MRP in the mare, and thus bringing added value to the current knowledge. Further integrative and functional analyses will help to confirm the role of these molecules in uEVs during MRP in the mare.

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“…Indeed, TAC represents the overall antioxidative activity of the organism and is also linked to lipid peroxidation to some extent (Li et al, 2019;Moradi, Goodarzi, et al, 2021). Consistently, recent investigations indicated that the supplementation of Proline enhances the activity of specific intracellular enzymatic and non-enzymatic antioxidants (Liu et al, 2021;Ponrasu et al, 2013).…”

Section: Discussionmentioning

confidence: 81%

Beneficial effect of L‐ Proline supplementation on the quality of human spermatozoa

Moradi

Hashemian

et al. 2022

Andrologia

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L-Proline is a natural anti-oxidative and osmoprotectant agent, playing a versatile role in cell metabolism and physiology. The present study aimed to explore the antioxidant effects of L-Proline on human sperm function during incubation. Thirty healthy, normozoospermic men (27-40 years) were enrolled. Sperm samples were incubated in an unsupplemented sperm medium (control group), or supplemented with L-Proline (1, 2 and 4 mmol/L) to evaluate its effect during 0, 1, 4 and 24 h of incubation. Sperm were assessed in terms of motility, viability, morphology, chromatin and DNA integrity. Moreover, the levels of reactive oxygen species (ROS), malondialdehyde (MDA), and total antioxidant capacity (TAC) were determined in the sperm medium. The results indicated that 2 mmol/L of L-Proline significantly improved the maintenance of sperm motility, viability, normal morphology, chromatin and DNA integrity, and TAC levels compared to the control group during 24 h of incubation (p < 0.05). However, 1 and 4 mmol/L of L-Proline could not significantly preserve sperm parameters, chromatin quality, and antioxidant status during different incubation times compared to the control group (p > 0.05). Collectively, the inclusion of L-Proline (2 mmol/L) in the human sperm medium maintains sperm parameters and chromatin quality probably by modulating the oxidative status.

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L-Proline Activates Mammalian Target of Rapamycin Complex 1 and Modulates Redox Environment in Porcine Trophectoderm Cells

Cited by 9 publications

References 61 publications

The Multifaceted Roles of Proline in Cell Behavior

The Multifaceted Roles of Proline in Cell Behavior

Uterine extracellular vesicles as multi-signal messengers during maternal recognition of pregnancy in the mare

Beneficial effect of L‐ Proline supplementation on the quality of human spermatozoa

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