l-Theanine attenuates neointimal hyperplasia via suppression of vascular smooth muscle cell phenotypic modulation

Bi, Aijing; Hang, Qiyun; Huang, Youfang; Zheng, Shuangning; Bi, Xiaowen; Zhang, Zhengping; Yin, Zhimin; Luo, Lan

doi:10.1016/j.jnutbio.2020.108398

Cited by 12 publications

(5 citation statements)

References 41 publications

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“…To further confirm the role of putrescine in regulating the synthetic phenotype of HASMCs, we used the PROMO database [ 31 ] to identify transcription factors regulating osteopontin (OPN) and epiregulin (EREG), two classical proteins of synthetic HASMCs that have been used as markers as reported previously [ 32 ] ( Figure 6 A ). Among the 35 transcription factors putatively transcribing both OPN and EREG, CCAAT/enhancer‐binding protein β (C/EBP β ) was predicted to be the most important.…”

Section: Resultsmentioning

confidence: 99%

Gasdermin D Deficiency in Vascular Smooth Muscle Cells Ameliorates Abdominal Aortic Aneurysm Through Reducing Putrescine Synthesis

et al. 2022

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Abdominal aortic aneurysm (AAA) is a common vascular disease associated with significant phenotypic alterations in vascular smooth muscle cells (VSMCs). Gasdermin D (GSDMD) is a pore‐forming effector of pyroptosis. In this study, the role of VSMC‐specific GSDMD in the phenotypic alteration of VSMCs and AAA formation is determined. Single‐cell transcriptome analyses reveal Gsdmd upregulation in aortic VSMCs in angiotensin (Ang) II‐induced AAA. VSMC‐specific Gsdmd deletion ameliorates Ang II‐induced AAA in apolipoprotein E (ApoE)−/− mice. Using untargeted metabolomic analysis, it is found that putrescine is significantly reduced in the plasma and aortic tissues of VSMC‐specific GSDMD deficient mice. High putrescine levels trigger a pro‐inflammatory phenotype in VSMCs and increase susceptibility to Ang II‐induced AAA formation in mice. In a population‐based study, a high level of putrescine in plasma is associated with the risk of AAA (p < 2.2 × 10−16), consistent with the animal data. Mechanistically, GSDMD enhances endoplasmic reticulum stress‐C/EBP homologous protein (CHOP) signaling, which in turn promotes the expression of ornithine decarboxylase 1 (ODC1), the enzyme responsible for increased putrescine levels. Treatment with the ODC1 inhibitor, difluoromethylornithine, reduces AAA formation in Ang II‐infused ApoE−/− mice. The findings suggest that putrescine is a potential biomarker and target for AAA treatment.

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Section: Resultsmentioning

confidence: 99%

Gasdermin D Deficiency in Vascular Smooth Muscle Cells Ameliorates Abdominal Aortic Aneurysm Through Reducing Putrescine Synthesis

et al. 2022

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“…L-theanine (γ-glutamylethylamide) is a non-protein amino acid found principally in the tea plant and mushrooms [ 5 ]. It shows beneficial effects on various nutritional and metabolic diseases in humans, and it has antioxidative [ 6 ], neuroprotective [ 7 , 8 ], hepatoprotective [ 9 ], anti-tumor [ 10 ] and anti-restenosis effects [ 11 ]. L-theanine can also reduce the risk of the development of diabetes mellitus type 2 (T2DM) [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

L-theanine prevents progression of nonalcoholic hepatic steatosis by regulating hepatocyte lipid metabolic pathways via the CaMKKβ-AMPK signaling pathway

Jiang

Liu

et al. 2022

Nutr Metab (Lond)

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Background L-theanine, a non-protein amino acid was found principally in the green tea, has been previously shown to exhibit potent anti-obesity property and hepatoprotective effect. Herein, we investigated the effects of L-theanine on alleviating nonalcoholic hepatic steatosis in vitro and in vivo, and explored the underlying molecular mechanism. Methods In vitro, HepG2 and AML12 cells were treated with 500 μM oleic acid (OA) or treated with OA accompanied by L-theanine. In vivo, C57BL/6J mice were fed with normal control diet (NCD), high‐fat diet (HFD), or HFD along with L-theanine for 16 weeks. The levels of triglycerides (TG), accumulation of lipid droplets and the expression of genes related to hepatocyte lipid metabolic pathways were detected in vitro and in vivo. Results Our data indicated that, in vivo, L-theanine significantly reduced body weight, hepatic steatosis, serum levels of alanine transaminase (ALT), aspartate transaminase (AST), TG and LDL cholesterol (LDL-C) in HFD-induced nonalcoholic fatty liver disease (NAFLD) mice. In vitro, L-theanine also significantly alleviated OA induced hepatocytes steatosis. Mechanic studies showed that L-theanine significantly inhibited the nucleus translocation of sterol regulatory element binding protein 1c (SREBP-1c) through AMPK-mTOR signaling pathway, thereby contributing to the reduction of fatty acid synthesis. We also identified that L-theanine enhanced fatty acid β-oxidation by increasing the expression of peroxisome proliferator–activated receptor α (PPARα) and carnitine palmitoyltransferase-1 A (CPT1A) through AMP-activated protein kinase (AMPK). Furthermore, our study indicated that L-theanine can active AMPK through its upstream kinase Calmodulin-dependent protein kinase kinase-β (CaMKKβ). Conclusions Taken together, our findings suggested that L-theanine alleviates nonalcoholic hepatic steatosis by regulating hepatocyte lipid metabolic pathways via the CaMKKβ-AMPK signaling pathway.

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“…L-theanine, a natural antioxidant mainly found in green tea, has been shown to inhibit angiogenesis [18]and can be used to prevent the occurrence of tumors such as breast cancer. Angiogenesis is a critical factor in tumor growth and spread.…”

Section: L-theanine Inhibits Tumor Angiogenesismentioning

confidence: 99%

L-theanine mechanism and application in the treatment of breast cancer

Li,

Cai,

Chen

et al. 2024

TNS

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Breast cancer has been recognized as the leading cancer worldwide and is one of the most common diseases among women, with an incidence rate of up to 7.7%. The pathogenesis of breast cancer can be categorized into congenital and acquired factors, including epigenetics and mutations. The occurrence of breast cancer is closely related to acquired factors such as environment, emotions, and diet. Numerous studies have demonstrated that L-theanine in tea can intervene in the growth and spread of breast cancer cells, thus playing a role in the treatment and prevention of breast cancer. This article provides an overview of the current research on the prevention and treatment of breast cancer using L-theanine, and also prospects the future development and application of L-theanine.

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l-Theanine attenuates neointimal hyperplasia via suppression of vascular smooth muscle cell phenotypic modulation

Cited by 12 publications

References 41 publications

Gasdermin D Deficiency in Vascular Smooth Muscle Cells Ameliorates Abdominal Aortic Aneurysm Through Reducing Putrescine Synthesis

Gasdermin D Deficiency in Vascular Smooth Muscle Cells Ameliorates Abdominal Aortic Aneurysm Through Reducing Putrescine Synthesis

L-theanine prevents progression of nonalcoholic hepatic steatosis by regulating hepatocyte lipid metabolic pathways via the CaMKKβ-AMPK signaling pathway

L-theanine mechanism and application in the treatment of breast cancer

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