L-Tryptophan 2′,3′-Oxidase from Chromobacterium violaceum

Genet, Roger; Bénetti, Pierre-Henri; Hammadi, Akli; Ménèz, Andre

doi:10.1074/jbc.270.40.23540

Cited by 38 publications

(19 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Its structure is related to tryptophan and serotonin but the enzymatic production of α,β-dehydrotryptophan in humans has not yet been reported. However, the presence of Ltryptophan 2',3'-oxidase and related enzymes -capable of production of α,β-dehydrotryptophan (indole-3-acrylic acid) -has been described in some common bacteria which may populate the gut (see Roberts and Rosenfeld 1977, Zavala et al 1983, Genet et al 1995. Hence, the exogenous (gut flora) source of IAG cannot be excluded.…”

Section: Discussionmentioning

confidence: 99%

Is the presence of urinary indolyl-3-acryloylglycine associated with autism spectrum disorder?

Wright¹,

Brzozowski²,

Calvert³

et al. 2005

Dev Med Child Neurol

View full text Add to dashboard Cite

To test whether the presence of indolyl-3-acryloylglycine (IAG) is associated with autism, we analyzed urine from population-based, blinded cohorts. All children in York, UK with autism spectrum disorders (ASDs), diagnosed using ICD-10 research diagnostic criteria, were invited to participate. Fifty-six children on the autism spectrum (mean age 9y 8mo, SD 3y 8mo; 79% male) agreed to participate, as did 155 children without ASDs (mean age 10y, SD 3y 2mo; 54% male) in mainstream and special schools (56 of whom were age-, sex-, and school-matched to children with ASDs). IAG was found at similar levels in the urine of all children, whether IAG concentrations or IAG:creatinine ratios were compared. There was no significant difference between the ASD and the comparison group, and no difference between children at mainstream schools and those at special schools. There is no association between presence of IAG in urine and autism; therefore, it is unlikely to be of help either diagnostically or as a basis for recommending therapeutic intervention with dietary manipulation. The significance of the presence of IAG in urine has yet to be determined.

show abstract

Section: Discussionmentioning

confidence: 99%

Is the presence of urinary indolyl-3-acryloylglycine associated with autism spectrum disorder?

Wright¹,

Brzozowski²,

Calvert³

et al. 2005

Dev Med Child Neurol

View full text Add to dashboard Cite

show abstract

“…For example, this activity has been reported for the commensal microbiota of mackerel [128]. On the other hand, a tryptophan oxidase activity has been described as playing a role in the synthesis of the pigment violacein in the free living marine bacterium Chromobacterium violaceum [129]. Other violacein pigmented marine bacteria, such as Pseudoalteromonas luteoviolacea , have shown broad range l -amino acid oxidase activity [130].…”

Section: L-amino Acid Oxidasesmentioning

confidence: 99%

Finding New Enzymes from Bacterial Physiology: A Successful Approach Illustrated by the Detection of Novel Oxidases in Marinomonas mediterranea

2010

View full text Add to dashboard Cite

The identification and study of marine microorganisms with unique physiological traits can be a very powerful tool discovering novel enzymes of possible biotechnological interest. This approach can complement the enormous amount of data concerning gene diversity in marine environments offered by metagenomic analysis, and can help to place the activities associated with those sequences in the context of microbial cellular metabolism and physiology. Accordingly, the detection and isolation of microorganisms that may be a good source of enzymes is of great importance. Marinomonas mediterranea, for example, has proven to be one such useful microorganism. This Gram-negative marine bacterium was first selected because of the unusually high amounts of melanins synthesized in media containing the amino acid l-tyrosine. The study of its molecular biology has allowed the cloning of several genes encoding oxidases of biotechnological interest, particularly in white and red biotechnology. Characterization of the operon encoding the tyrosinase responsible for melanin synthesis revealed that a second gene in that operon encodes a protein, PpoB2, which is involved in copper transfer to tyrosinase. This finding made PpoB2 the first protein in the COG5486 group to which a physiological role has been assigned. Another enzyme of interest described in M. mediterranea is a multicopper oxidase encoding a membrane-associated enzyme that shows oxidative activity on a wide range of substrates typical of both laccases and tyrosinases. Finally, an enzyme very specific for l-lysine, which oxidises this amino acid in epsilon position and that has received a new EC number (1.4.3.20), has also been described for M. mediterranea. Overall, the studies carried out on this bacterium illustrate the power of exploring the physiology of selected microorganisms to discover novel enzymes of biotechnological relevance.

show abstract

“…LAAO may act as defence or attack weapons via H 2 O 2 [11] or as ideal molecular mechanisms for the acquisition of nitrogen from diverse amino acid sources [12]. In addition, researches have showed that LAAO affects the relation of tumor cells with the immune system [13] and is involved in violacein synthesis [14]. So far, LAAO was found to be applied as catalysts in bio-transformation [15] and as part of biosensors in determination of the different forms (D-or L-) of free amino acids [16].…”

Section: Nhmentioning

confidence: 99%

Isolation and Characterization of an L-Amino Acid Oxidase-Producing Marine Bacterium

Yu¹,

Qiao²,

Qiu³

et al. 2013

ENG

View full text Add to dashboard Cite

One marine bacterial strain, R3, has been newly isolated from the intertidal zone of Dinghai sea area. Measurements of a-keto acids and H 2 O 2 existing in fermentation supernatant were carried out to show that R3 can produce L-amino acid oxidase (LAAO) with a broad substrate specificity. Physiological and biochemical analysis showed that it can grow great at the conditions with sodium chloride concentration of 1.5% -3%, temperature of 15˚C -35˚C and pH of 6 -7. In addition, molecular identification of 16S rDNA was performed to show that R3 was proximal to Pseudoalteromonas spp. with the highest identity of 98.5% to Pseudoalteromonas rubra. Therefore, it was designated as Pseudoalteromonas sp. R3. Further studies are required to arrive at a better understanding of this LAAO and secure an application.

show abstract

L-Tryptophan 2′,3′-Oxidase from Chromobacterium violaceum

Cited by 38 publications

References 17 publications

Is the presence of urinary indolyl-3-acryloylglycine associated with autism spectrum disorder?

Is the presence of urinary indolyl-3-acryloylglycine associated with autism spectrum disorder?

Finding New Enzymes from Bacterial Physiology: A Successful Approach Illustrated by the Detection of Novel Oxidases in Marinomonas mediterranea

Isolation and Characterization of an L-Amino Acid Oxidase-Producing Marine Bacterium

Contact Info

Product

Resources

About