L-Type Ca2+ Channels in the Enteric Nervous System Mediate Oscillatory Cl- Secretion in Guinea Pig Colon

Nishikitani, Mariko; Yasuoka, Yukiko; Kawada, Hideaki; Kawahara, Katsumasa

doi:10.1620/tjem.211.151

Cited by 3 publications

(2 citation statements)

References 32 publications

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“…The available evidence suggests that parasympathetic innervations play an important role in the gut motility [ 23 ]. Studies have also indicated that Ach stored in the nonneuronal cells of the gut plays an important role in stimulating the smooth muscle contraction [ 24 ]. Furthermore, the muscarinic receptor innervations can also increase the secretion of mucus from goblet cells.…”

Section: Discussionmentioning

confidence: 99%

Preclinical Studies to Evaluate the Gut Stimulatory Activity of Aloe Musabbar

Tandur

Inamdar

Orfali

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. Constipation is a common functional gastrointestinal disorder. Medicines derived from nature are routinely used to treat it. The present study evaluates the gut stimulatory activity of Aloe musabbar (processed powder of Aloe vera) using in vitro and in vivo models for gut stimulatory activity. Materials and Methods. In vitro tests were conducted on isolated rat colon, guinea pig ileum, and rabbit jejunum, while in vivo study was performed using mice intestinal transit time. Aloe musabbar (A. musabbar) was tested at doses 0.2–200 mg/mL (in-vitro study) and 86.6 mg/kg (in vivo study). In vitro studies were done in the presence and absence of atropine sulphate (1 ng/ml). The results were statistically analyzed, and p < 0.05 was considered to indicate the significance. Results. A. musabbar exhibited dose-dependent increase in the smooth muscle contraction of isolated gut tissues. Presence of atropine minimized the contractile responses and shifted the dose-response curves towards the right-hand side. The intestinal transit time in mice was observed to be increased significantly ( p < 0.01 ) in A. musabbar-treated animals, when compared with normal animals. Conclusion. A mild smooth muscle contraction induced by A. musabbar suggests that it can stimulate intestinal bowel movement without causing spasms. The diminished responses in the presence of atropine indicated that the gut stimulatory activity could be mediated partially through parasympathetic innervations. More studies are needed to determine the precise mechanism of action including the specific active ingredient responsible for the gut stimulatory activity.

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Section: Discussionmentioning

confidence: 99%

Preclinical Studies to Evaluate the Gut Stimulatory Activity of Aloe Musabbar

Tandur

Inamdar

Orfali

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…These Ach molecules are not stored in the vesicles and are released by organic cation transporters. Their release is considered to produce slow and sustained gut stimulatory action 23 . In an earlier study, the role of calcium has been suggested for the T. chebula mediated stimulation in the gut smooth muscles and the action can be linked to the cholinergic innervations 11 .…”

Section: Discussionmentioning

confidence: 99%

Gut Stimulatory Effect of Terminalia chebula in Experimental Animal Models

Tandur¹,

Inamdar²,

Alamri³

et al. 2022

International J. of Pharmacology

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Background and Objective: Constipation is one of the universal gastrointestinal problems that affect people of all ages. Several medications are used to relieve constipation, but their use is associated with adverse effects. This study evaluated the gut stimulatory activity of Terminalia chebula powder extract using in vitro and in vivo experimental methods. Materials and Methods: The powder extract of Terminalia chebula (T. Chebula) was reconstituted in distilled water. In vitro gut motility studies were performed on isolated rat colon, Guinea pig ileum and rabbit jejunum, while in vivo study was conducted on mice to determine the charcoal transit time. The in vitro studies were conducted in the presence and absence of atropine. The results were statistically analysed and p<0.05 was considered to indicate significance. Results: The observations indicated that T. chebula extracts produced dose-dependent stimulation of the smooth muscles of the colon, ileum and jejunum and the magnitude was found to be less than acetylcholine (Ach). The doseresponse curves of both T. chebula and Ach were found to be shifted to the right-hand side when tested in presence of atropine. The intestinal transit time for a charcoal meal in mice significantly increased (p<0.05) when compared with normal animals. Conclusion: The mild stimulatory action induced by T. chebula could be useful in relieving the defective gastric motility without affecting the functional integrity of the digestive system. However, more studies are needed to establish the safety and efficacy including the mechanism of action of T. chebula.

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R-Type Calcium Channels Are Crucial for Semaphorin 3A–Induced DRG Axon Growth Cone Collapse

Treinys

Kaselis

Jover³

et al. 2014

PLoS ONE

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Semaphorin 3A (Sema3A) is a secreted protein involved in axon path-finding during nervous system development. Calcium signaling plays an important role during axonal growth in response to different guidance cues; however it remains unclear whether this is also the case for Sema3A. In this study we used intracellular calcium imaging to figure out whether Sema3A-induced growth cone collapse is a Ca2+ dependent process. Intracellular Ca2+ imaging results using Fura-2 AM showed Ca2+ increase in E15 mice dorsal root ganglia neurons upon Sema3A treatment. Consequently we analyzed Sema3A effect on growth cones after blocking or modifying intracellular and extracellular Ca2+ channels that are expressed in E15 mouse embryos. Our results demonstrate that Sema3A increased growth cone collapse rate is blocked by the non-selective R- and T- type Ca2+ channel blocker NiCl2 and by the selective R-type Ca2+ channel blocker SNX482. These Ca2+ channel blockers consistently decreased the Sema3A-induced intracellular Ca2+ concentration elevation. Overall, our results demonstrate that Sema3A-induced growth cone collapses are intimately related with increase in intracellular calcium concentration mediated by R-type calcium channels.

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L-Type Ca2+ Channels in the Enteric Nervous System Mediate Oscillatory Cl- Secretion in Guinea Pig Colon

Abstract: The enteric nervous system regulates epithelial ion and fluid secretion. Our previous study has shown that the low (0.

Cited by 3 publications

References 32 publications

Preclinical Studies to Evaluate the Gut Stimulatory Activity of Aloe Musabbar

Preclinical Studies to Evaluate the Gut Stimulatory Activity of Aloe Musabbar

Gut Stimulatory Effect of Terminalia chebula in Experimental Animal Models

R-Type Calcium Channels Are Crucial for Semaphorin 3A–Induced DRG Axon Growth Cone Collapse

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