L1CAM, INP10, P-cadherin, tPA and ITGB4 over-expression in malignant pleural mesotheliomas revealed by combined use of cDNA and tissue microarray

Kettunen, Eeva; Nicholson, Andrew G.; Nagy, Balázs; Wikman, Harriet; Seppänen, Janne; Stjernvall, Tuula; Ollikainen, Tiina; Kinnula, Vuokko L.; Nordling, Stig; Hollmén, Jaakko; Anttila, Sisko; Knuutila, Sakari

doi:10.1093/carcin/bgh276

Cited by 38 publications

(23 citation statements)

References 42 publications

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“…Unexpectedly, genes encoding calretinin, vascular endothelial growth factor (VEGF) receptor and mesothelin proteins overexpressed in mesothelioma were not differentially expressed. However, recent studies showed that these are also expressed in normal mesothelial cells [103][104][105].…”

Section: Figurementioning

confidence: 99%

Malignant pleural mesothelioma: history, controversy and future of a manmade epidemic

2015

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Asbestos is the term for a family of naturally occurring minerals that have been used on a small scale since ancient times. Industrialisation demanded increased mining and refining in the 20th century, and in 1960, Wagner, Sleggs and Marchand from South Africa linked asbestos to mesothelioma, paving the way to the current knowledge of the aetiology, epidemiology and biology of malignant pleural mesothelioma. Pleural mesothelioma is one of the most lethal cancers, with increasing incidence worldwide. This review will give some snapshots of the history of pleural mesothelioma discovery, and the body of epidemiological and biological research, including some of the controversies and unresolved questions. Translational research is currently unravelling novel circulating biomarkers for earlier diagnosis and novel treatment targets. Current breakthrough discoveries of clinically promising noninvasive biomarkers, such as the 13-protein signature, microRNAs and the BAP1 mesothelioma/cancer syndrome, are highlighted. The asbestos history is a lesson to not be repeated, but here we also review recent in vivo and in vitro studies showing that manmade carbon nanofibres could pose a similar danger to human health. This should be taken seriously by regulatory bodies to ensure thorough testing of novel materials before release in the society. @ERSpublications Malignant pleural mesothelioma is a cancer with increasing death tolls due to the past and present use of asbestos http://ow.ly/DhA2y

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Section: Figurementioning

confidence: 99%

Malignant pleural mesothelioma: history, controversy and future of a manmade epidemic

2015

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“…We used two kind of gene expression determination, SYBRGreen I (for β-globin, PL2A and c-myc) and primer-probe system (CD24) [17][18][19]. CD24 expression was determined according to a previously described method [17].…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

“…CD24 expression was determined according to a previously described method [17]. c-myc and PL2A gene specific primers were designed and synthesized by TIB MOLBIOL (Berlin, Germany) [18,20]. The house-keeping gene β-globin expression was measured and used for normalization of the gene expression results.…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

“…Quantitative polymerase chain reaction was performed using LightCycler (Roche) for determination of β-globin, PL2A and c-myc expressions, each PCR-mix consisted of 1 μl of DNA Master SYBR Green I mix (LC FastStart DNA Master SYBR Green I kit, Roche), in the case of CD24 expression measurements, 1 μl of FastSart DNA Master mix (LC FastStart DNA Master HybProbe, Roche), 1.2 mM MgCl 2 , and 0.25 pmol of primers and probes [14,[17][18][19]. The amplification program included an initial denaturation at 95°C with 10 min hold, followed by 40 cycles with denaturation at 95°C with 10 s hold, annealing at 56°C and 65°C with 5 s hold and extension at 72°C with 10 s hold.…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

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Overexpression of CD24, c-myc and Phospholipase 2A in Prostate Cancer Tissue Samples Obtained by Needle Biopsy

Nagy

Szendrői

Romics

2008

Pathol. Oncol. Res.

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Altered CD24, c-myc and phospholipase 2a expression was reported in different cancers. Our aim was to measure the expression of these genes in prostate cancer tissues, and compare it to non-cancerous samples. Prostate tissue samples were collected by needle biopsy from 20 prostate cancer (PCA) and 11 benign prostate hyperplasic (BPH) patients. RNA was isolated; cDNA synthetized, CD24, c-myc and phospholipase 2A (PL2A) expressions were determined by quantitative real-time PCR method. The expression of beta-globin gene was measured for normalization of the gene expression results. Serum prostate specific antigen (PSA) levels were determined by microparticle enzyme immunoassay (MEIA) method. PSA levels were significantly different between the PCA and BPH groups, 252.37 +/- 308.33 ng/ml vs. 3.5 +/- 2.14 ng/ml (p = 0.001), respectively. CD24 expression was 988.86 +/- 3041 ng/microl in prostate tumor and 4.00 +/- 4.25 ng/microl in the BPH group (p = 0.035). The c-myc expression was 88.32 +/- 11.93 ng/microl in the prostate tumor and 17.08 +/- 21.75 ng/microl in the BPH group (p = 0.02), and the PL2A 31.36 +/- 67.02 ng/microl was in PCA and 5.56 +/- 14.08 ng/microl in BPH (p = 0.025). Gleason's scores showed correlation with c-myc (p = 0.019) and PSA (p = 0.033) levels. Overexpression of PL2A, CD24 and c-myc was observed in prostate cancer samples using quantitative real-time PCR method.

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“…[10][11][12][13][14]. Prognostic prediction based on gene expression profiles has been attempted by two groups, but the ''prognostic gene lists'' have shown little or no overlap (13,15,16).…”

Section: Introductionmentioning

confidence: 99%

Global Gene Expression Profiling of Pleural Mesotheliomas: Overexpression of Aurora Kinases and P16/CDKN2A Deletion as Prognostic Factors and Critical Evaluation of Microarray-Based Prognostic Prediction

López‐Ríos¹,

Chuai

Flores³

et al. 2006

Cancer Research

232

187

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Most gene expression profiling studies of mesothelioma have been based on relatively small sample numbers, limiting their statistical power. We did Affymetrix U133A microarray analysis on 99 pleural mesotheliomas, in which multivariate analysis showed advanced-stage, sarcomatous histology and P16/CDKN2A homozygous deletion to be significant independent adverse prognostic factors. Comparison of the expression profiles of epithelioid versus sarcomatous mesotheliomas identified many genes significantly overexpressed among the former, including previously unrecognized ones, such as uroplakins and kallikrein 11, both confirmed by immunohistochemistry. Examination of the gene expression correlates of survival showed that more aggressive mesotheliomas expressed higher levels of Aurora kinases A and B and functionally related genes involved in mitosis and cell cycle control. Independent confirmation of the negative effect of Aurora kinase B was obtained by immunohistochemistry in a separate patient cohort. A role for Aurora kinases in the aggressive behavior of mesotheliomas is of potential clinical interest because of the recent development of small-molecule inhibitors. We then used our data to develop microarray-based predictors of 1 year survival; these achieved a maximal accuracy of 68% in cross-validation. However, this was inferior to prognostic prediction based on standard clinicopathologic variables and P16/CDNK2A status (accuracy, 73%), and adding the microarray model to the latter did not improve overall accuracy. Finally, we evaluated three recently published microarray-based outcome prediction models, but their accuracies ranged from 63% to 67%, consistently lower than reported. Gene expression profiling of mesotheliomas is an important discovery tool, but its power in clinical prognostication has been overestimated.

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L1CAM, INP10, P-cadherin, tPA and ITGB4 over-expression in malignant pleural mesotheliomas revealed by combined use of cDNA and tissue microarray

Cited by 38 publications

References 42 publications

Malignant pleural mesothelioma: history, controversy and future of a manmade epidemic

Malignant pleural mesothelioma: history, controversy and future of a manmade epidemic

Overexpression of CD24, c-myc and Phospholipase 2A in Prostate Cancer Tissue Samples Obtained by Needle Biopsy

Global Gene Expression Profiling of Pleural Mesotheliomas: Overexpression of Aurora Kinases and P16/CDKN2A Deletion as Prognostic Factors and Critical Evaluation of Microarray-Based Prognostic Prediction

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