“…Streptococcus mutans is the most prevalent microorganism in dental biofilm [ 23 , 24 , 25 , 26 ], this Gram-positive facultative anaerobic bacterium has the capacity for acidogenicity, aciduricity, acid tolerance, and ability to synthesize the extracellular matrix of the dental plaque [ 27 , 28 , 29 ]. Primary responsible for the generation of dental caries [ 30 , 31 , 32 , 33 ], the second most prevalent chronic disease worldwide [ 34 ] considered as the higher global health problem since it affects multiple groups old [ 35 , 36 ]. Another important microorganism is Enterococcus faecalis , a Gram-positive facultative anaerobic bacterium typical of the human intestinal microbiota but can be found infrequently in the oral cavity [ 30 , 37 ].…”