Synchronous losses of biological, linguistic, and cultural diversity are contributing to processes of biocultural homogenization, a persistent downgrading in how people perceive biodiversity, environmental, and cultural conditions, and what they consider as normal. Some have linked biocultural homogenization with neoliberal practices that emphasize standardization and efficiencies in order to enhance capital accumulation. In Chile, which is highly centralized, urban, and centered around neoliberal development, biocultural homogenization is especially concerning, as it may lower support for conservation. Thus, when thinking about conservation-based development in Patagonia, locally driven initiatives that foster biocultural reawakening, democratize science, and catalyze sustainable development, might help address biocultural homogenization and build conservation support. This mixed-methods, intrinsic case study explored three initiatives underway in the Aysén and Magallanes regions of Chile that address some of these outcomes: (1) Subantarctic Natural Laboratories; (2) 3-Hs Biocultural Ethic and FEP Cycle Approach; and (3) Scientific Tourism Collaborative Learning Networks. We sought to better understand their methods and implementations and explore how their integration might strengthen conservation-based development in Patagonia, through ethical travel experiences rooted in the bioculture of local communities. Results suggest promise for a combined approach; thus, additional research and consideration are merited.