LAB bacteriocin applications in the last decade

Abstract

In the early 2000s, the expectations about bacteriocins produced by lactic acid bacteria (LABs) were aimed at food applications. However, the effectiveness of bacteriocins against undesirable microorganisms opened endless possibilities for innovative research. In the present review, we collected a database including 429 published papers and 245 granted patents (from 2004 to 2015). Based on bibliometric analysis, the progress of bacteriocin research in the last 11 years was discussed in detail. It was found that 164 patents were granted in 2010-2015, which is equivalent to 60% in comparison with previous years (i.e. only 81 patents were granted in [2004][2005][2006][2007][2008][2009]. Currently, the research on bacteriocins is still gaining importance. In the realm of therapeutic strategies, about a 37% of the published research was focused on biomedical applications in the last decade. This vein of research is currently seeking for alternative solutions to problems such as cancer, systemic infections, oral-care, vaginal infections, contraception and skincare. On the other hand, food preservation, bio-nanomaterial and veterinary applications represent 29%, 25% and 9%, respectively. All this technology is being applied and will surely grow in the future, since about 31% of the patents granted since 2004 are focused on the biomedical area, 29% on food preservation, 5% on veterinary use; whereas 13% and 16% correspond to patents granted on production-purification systems and recombinant proteins or molecular modifications in the producer strains. This review contributes to the analysis of recent LAB bacteriocin applications and their role in safety, quality and improvement of human health.

Synthesis, antimicrobial activity and conformational analysis of the class IIa bacteriocin pediocin PA-1 and analogs thereof

“…The Cys9–Cys14 bond is conserved among the class IIa bacteriocins, as is the N-terminal YGNGV sequence, both structures being signatures of this family 26 . Despite recent progress in its production by recombinant technologies 32 , pure bioactive pediocin PA-1 is not commercially available at present time and commercial crude fermentation products such as ALTA 2341 ® (Quest International) 9 or freeze-dried pediocin-producing cultures like CHOOZIT ™ FLAV 43 (Danisco) must be used 33 . Total chemical synthesis of bioactive pediocin PA-1 has been attempted in the past but with limited success, due to yields below 1% after multiple purification steps 20 , 34 .…”

Characterization of a potential probiotic bacterium Lactococcus raffinolactis WiKim0068 isolated from fermented vegetable using genomic and in vitro analyses

“…Significantly, bacteriocins production have been regarded as an important feature in the selection of probiotic strains. These genes were associated with the presence of useful probiotic characteristics, which play important roles in the food and pharmaceutical industries [15][16][17]. 2).…”

Effect of Acetic Acid on Bacteriocin Production by Gram-Positive

“…Therefore, bacteriocin has good performance in inhibiting the growth of a variety of pathogenic microorganisms and food spoilage bacteria. In the past decade or so, articles on the application of bacteriocins in biomedical fields accounted for most of those published [1], with the majority of these related to the study of bacteriocins produced by Gram-negative bacteria. Bacteriocin research on Gram-positive bacteria is still in its infancy and is mainly concentrated on lactic acid bacteria.…”