LAB İzolasyonu ve Silaj Fermentasyon Yeteneklerinin Belirlenmesi

Abstract

Usage of high quality forage that enhances the production efficiency in the livestock industry is a quite important issue. During a famine period or when it is unavailable to reach fresh forage due to seasonal factors, the ensiled forage becomes a vital role for feeding ruminants. Lactic Acid Bacteria (LAB) is the most important microorganisms in silage for desired fermentation. In this work, LAB isolation in epiphytic flora from diverse field forage crops and ecologies in Turkey were made in order to determine their fermentative capacity and to develop a high-quality silage inoculant. For this purpose, the forage crops were collected from several rangelands from different altitudes of 39 to 1516 m and isolations were made from both fresh forage and ensiled material. Total acid production capacities of isolates were determined and 70 isolates were selected in terms of acid zone creation in growth media containing CaCO 3. Then, LA production levels in MRS broth media of isolates were determined and final 10 isolates were selected for inoculation trials. Also, morphological, physiologic, biochemical properties of selected 10 isolates were analyzed and identified by the BIOLOG kit on species level. Isolates that have outstanding properties about lactic acid productivity are left to use further inoculation research work.